Kinetic constants of α-methyl-d-glucoside transport in the chick small intestine during perinatal development

Moreno, María Alejandra; Otero, María Pilar Fernández; Tur, Josep A.; Planas, Joana M.; Esteban, Susana

doi:10.1016/s0047-6374(96)01798-8

Cited by 10 publications

(7 citation statements)

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“…Our results showed that in general the expression levels of SGLT1 were up-regulated in the first week; however, the regulatory mechanism behind these increases in SGLT1 levels is unknown. If it is correct that SGLT1 is regulated at the transcriptional level, our results could explain the reports of Moreno and colleagues who reported that the glucose transport rate was highest right after hatching but decreased thereafter and was lowest in older birds (Moreno, Otero, Tur, Planas, & Esteban, 1996). If it is correct that SGLT1 is regulated at the transcriptional level, our results could explain the reports of Moreno and colleagues who reported that the glucose transport rate was highest right after hatching but decreased thereafter and was lowest in older birds (Moreno, Otero, Tur, Planas, & Esteban, 1996).…”

Section: Discussionsupporting

confidence: 81%

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Cheng

Yuan

et al. 2019

Animal Physiology Nutrition

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In the chicken small intestine, glucose is mainly transported by the apically located sodium/glucose cotransporter 1 (SGLT1) and the basolaterally located glucose transporter 2 (GLUT2). Fructose is transported by the apically located glucose transporter 5 (GLUT5) and similarly by GLUT2. During the early post‐hatching period, the intestinal villus surface area (VSA) should be considered as an important factor related to the monosaccharide absorption capacity. Our objective here was to study intestinal monosaccharide absorption by analyzing the effects of age, diet, and breed on monosaccharide transporters and the VSA. The mRNA expression patterns of SGLT1, GLUT2 and GLUT5 genes in broiler and layer chickens were measured from the day of hatching to day 28 using the absolute quantitative real‐time PCR. Both the intestinal mRNA expression levels of these genes and the VSA were affected by age. The mRNA expression levels of SGLT1 and GLUT2 were significantly increased from day 1 to day 3 and then decreased from day 3 to day 28. The expression levels of GLUT5 decreased from day 1 to day 7. The broiler chickens VSAs were significantly larger than those of the layer chickens from days 7 to 28. The effect of diet on the gene expression patterns of these monosaccharide transporters and the VSA were not significant. Our results suggest that the expression levels of these monosaccharide transporters are increased rapidly at the beginning of intestinal growth to meet the demands for monosaccharides to support the fast growth of the chick before day 7. As intestinal maturation and VSA increased, the expression levels of these monosaccharide genes decreased to a certain expression level to maintain the intestinal transport capacity and the absorption balance of all other nutrients.

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Section: Discussionsupporting

confidence: 81%

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Cheng

Yuan

et al. 2019

Animal Physiology Nutrition

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“…Second, the expression of rBAT and B 0 AT, existing in the small intestine, was not detected in the IPEC-J2 cells. Similarly, there is evidence that system-B 0 specific amino acid uptake activity was absent in rat crypt-like cell line IEC-17 but was higher in less differentiated cells such as a Caco-2 cell line (Moreno et al 1996;Costa et al 2000). In addition, the expression of B 0 AT1 was demonstrated in enterocyte-specific pattern, but not in any other type of intestinal epithelial cells (Bröer et al 2004).…”

Section: Discussionmentioning

confidence: 92%

Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways

et al. 2014

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Leucine has been shown to influence intestinal protein metabolism, cell proliferation and migration. Furthermore, our previous study demonstrated that branched-chain amino acids could modulate the intestinal amino acid and peptide transporters in vivo. As the possible mechanisms are still largely unknown, in the present work, we studied the transcriptional and translational regulation of leucine on amino acid transporter production in IPEC-J2 cells and the signaling pathways involved. Treatment of IPEC-J2 cells with 7.5 mM leucine enhanced the mRNA expression of the Na(+)-neutral AA exchanger 2 (ASCT2) and 4F2 heavy chain (4F2hc) and caused an increase in ASCT2 protein expression. Leucine also activated phosphorylation of 4E-BP1 and eIF4E through the phosphorylation of mTOR, Akt and ERK signaling pathways in IPEC-J2 cells. Pre-treatment of IPEC-J2 cells with inhibitors of mTOR and Akt (rapamycin and wortmannin) or an inhibitor of ERK (PD098059) for 30 min before leucine treatment attenuated the positive effect of leucine in enhancing the protein abundance of ASCT2. These results demonstrate that leucine could up-regulate the expression of the amino acid transporters (ASCT2) through transcriptional and translational regulation by ERK and PI3K/Akt/mTOR activation.

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“…At hatch, intestinal morphology (16,30), digestive function (27), and nutrient transport capacity (2) have attained a state of complete development. The maximal capacity per tissue weight to transport monosaccharides is reached the day after hatch and declines afterward until attainment of adult levels at the age of 5 wk (18,25). This pattern occurs not only in chickens (19,29) but also in various species of mammals (2,23).…”

Section: Discussionmentioning

confidence: 97%

“…In chickens, Na ϩ -D-glucose cotransport is already detectable in the intestine during the last days of the embryonic period (18). At hatch, intestinal morphology (16,30), digestive function (27), and nutrient transport capacity (2) have attained a state of complete development.…”

Section: Discussionmentioning

confidence: 99%

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Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Barfull

Garriga

Mitjans

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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To evaluate the effect of age on sugar transport, we determined the uptake of methyl α-d-glucopyranoside and the abundance of the Na+-d-glucose cotransporter (SGLT1) in jejunal brush-border membrane (BBM) vesicles of 2-day- and 5-wk-old chickens. Methyl α-d-glucopyranoside transport per BBM protein was 40% lower in adults than in newly hatched chickens. This finding was matched by parallel declines in site density of SGLT1, which were detected by Western blot. The immunohistochemical study showed that SGLT1 was exclusively located in the BBM of enterocytes along the entire villus and was absent in the crypt in both age groups, and there was an 11-fold increase in the total absorptive area during development. Northern blot studies of the abundance of SGLT1 mRNA showed similar levels for the groups studied. We conclude that the age-related decline in Na+-dependent hexose transport per unit of BBM protein in the chicken jejunum is due to a reduction in the density of SGLT1 cotransporter and is regulated by a posttranscriptional mechanism.

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Kinetic constants of α-methyl-d-glucoside transport in the chick small intestine during perinatal development

Cited by 10 publications

References 20 publications

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

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Kinetic constants of α-methyl-d-glucoside transport in the chick small intestine during perinatal development

Cited by 10 publications

References 20 publications

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Age‐related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens

Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways

Ontogenetic expression and regulation of Na+-d-glucose cotransporter in jejunum of domestic chicken

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Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken