Kinetic differences of purified laccases from six Pleurotus ostreatus strains

Tinoco,; Pickard,; Vazquez-Duhalt,

doi:10.1046/j.1472-765x.2001.00913.x

Cited by 118 publications

(75 citation statements)

References 27 publications

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“…The top PEG-rich phase which contained the partially purified enzyme (Lac ATPS) was then used for the oxidation experiments. Enzymatic activity and total protein was estimated at the end of the purification protocol, as described by Tinoco et al [19] and Bradford [20], respectively.…”

Section: Partial Purification Of Laccase From a Bisporus Using Aqueomentioning

confidence: 99%

Oxidation of Polycyclic Aromatic Hydrocarbons using Partially Purified Laccase from Residual Compost of Agaricus bisporus

et al. 2011

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Laccase partially purified from residual compost of Agaricus bisporus by an aqueous two-phase system (Lac ATPS) was used in degrading polycyclic aromatic hydrocarbons: fluorene (Flu), phenanthrene (Phe), anthracene (Ant), benzo[a]pyrene (BaP), and benzo[a]anthracene (BaA). The capacity of the enzyme to oxidize polyaromatic compounds was compared to that of the crude laccase extract (CE). After treatment of 72 h, Lac ATPS and CE were not capable of oxidizing Flu and Phe, while Ant, BaP, and BaA were oxidized, resulting in percentages of oxidation of 11.2 ± 1, 26 ± 2, and 11.7 ± 4 % with CE, respectively. When Lac ATPS was used, the following percentages of oxidation were obtained: 11.4 ± 3 % for Ant, 34 ± 0.1 % for BaP, and 13.6 ± 2 % for BaA. The results reported here demonstrate the potential application of Lac ATPS for the oxidation of polycyclic aromatic hydrocarbons.

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Section: Partial Purification Of Laccase From a Bisporus Using Aqueomentioning

confidence: 99%

Oxidation of Polycyclic Aromatic Hydrocarbons using Partially Purified Laccase from Residual Compost of Agaricus bisporus

et al. 2011

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“…Decolorization percentages were calculated by following the methodology reported by Morales et al, 2016. Laccase activity was determined following the protocol reported by Tinoco et al, 2001. Manganese peroxidase (MnP) activity was determined following the protocol reported by Quevedo-Hidalgo et al, 2015. …”

Section: Experimental Designmentioning

confidence: 99%

“…Both assays were performed at 120 rpm, and the evaluation time was 72 and 216 h for MG and CV respectively (this time was selected in function to the decoloration observed where the media was almost without dye in each case). Periodic sampling was performed to determine the percentage of decolorization, laccase activity (Tinoco et al, 2001), pH, and reducing sugars (Miller 1959). On the other hand, some production parameters (e.g.…”

Section: Removal Experimentsmentioning

confidence: 99%

Partial removal and detoxification of Malachite Green and Crystal Violet from laboratory artificially contaminated water by Pleurotus ostreatus

et al. 2016

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The triphenylmethane Malachite Green (MG) and Crystal Violet (CV) dyes are cationic dyes and mix with domestic wastewater when dumped; increasing, among others, the chemical and biological oxygen demand and can cause acute toxicity at different trophic levels. Promoting the removal (decolorization) of MG and CV, and laccase activity (54.8 ± 8.9 and 30.6 ± 2.9 UL -1 respectively) by using P. ostreatus viable biomass needed parameters such as pH (4.5 and 6.0), temperature ). In adsorption studies, it was showed that an acidic pH favors the adsorption of both dyes and the model of pseudo-second order describes best the phenomenon of adsorption. Finally, the germination index (GI), using Lactuca sativa seeds for the initial dyes solutions, was < 50%; demonstrating its high phytotoxic effect. When dye solutions were treated with viable biomass, the GI increased, leaving open the possibility to perform future research to determine if the aqueous solutions, post-treated with P. ostreatus, could be used in treatments that generate less toxic water which could be used in processes that do not require potable water.

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“…One unit of laccase activity was defined as 1 µmol ABTS + formed per minute (Bourbonnais et al 1997, Tinoco et al 2001). …”

Section: Enzymatic Assaysmentioning

confidence: 99%

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

et al. 2014

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Floriculture is a vital agro-industrial sector in the Colombian economy; the export of flowers positively impacts employment and the balance of trade. However, this industry could negatively impact the environment if its waste products are not handled properly. These flower residues, rich in lignin, hemicellulose and cellulose, could be a cost-effective raw material to produce enzymes. Here, we evaluate the production of lignocellulolytic enzymes by degradation of Chrysanthemum and Rosa residues using Pleurotus ostreatus, and manganese sulfate and copper sulfate as inductors. From the two residues, we obtained laccase, manganese peroxidase, endoglucanase, exoglucanase, and β-glucosidase. The use of inductors, favored all enzyme activities except for β-glucosidase. The enzymes that displayed the highest activity were laccase (4,693.4 U/L and 2,640 U/L from the residues of Chrysanthemum and Rosa, respectively) and β-glucosidase (9,513 U/L and 6,811.9 U/L). The enzyme that showed the lowest activity was endoglucanase (11.5 U/L and 15.4 U/L). Under the conditions evaluated, the best substrate for enzyme production is Chrysanthemum wastes; the extracts obtained had higher enzymatic activity than the extracts from Rosa residues.

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Kinetic differences of purified laccases from six Pleurotus ostreatus strains

Abstract: The different enzymatic properties of laccases from various P. ostreatus strains should be considered for a potential industrial or environmental application.

Cited by 118 publications

References 27 publications

Oxidation of Polycyclic Aromatic Hydrocarbons using Partially Purified Laccase from Residual Compost of Agaricus bisporus

Oxidation of Polycyclic Aromatic Hydrocarbons using Partially Purified Laccase from Residual Compost of Agaricus bisporus

Partial removal and detoxification of Malachite Green and Crystal Violet from laboratory artificially contaminated water by Pleurotus ostreatus

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

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