2015
DOI: 10.3109/10715762.2015.1017478
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Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines

Abstract: Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a s… Show more

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Cited by 37 publications
(25 citation statements)
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“…[41] The bleaching of CB (50 × 10 −6 m) at 640 nm within 300 min (Figure 2e,f) showed that CeO 2−x nanorods catalyze the oxidation of bromide to HOBr/OBr − . [41] The bleaching of CB (50 × 10 −6 m) at 640 nm within 300 min (Figure 2e,f) showed that CeO 2−x nanorods catalyze the oxidation of bromide to HOBr/OBr − .…”
Section: Doi: 101002/adma201603823mentioning
confidence: 97%
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“…[41] The bleaching of CB (50 × 10 −6 m) at 640 nm within 300 min (Figure 2e,f) showed that CeO 2−x nanorods catalyze the oxidation of bromide to HOBr/OBr − . [41] The bleaching of CB (50 × 10 −6 m) at 640 nm within 300 min (Figure 2e,f) showed that CeO 2−x nanorods catalyze the oxidation of bromide to HOBr/OBr − .…”
Section: Doi: 101002/adma201603823mentioning
confidence: 97%
“…The catalytic activity of the CeO 2−x nanorods showed a pH optimum around pH 5.6, and it decreased with increasing pH. g) Illustration of the oxidative degradation of CB [41] catalyzed by CeO 2−x nanorods. This can be rationalized based on: i) the oxidation potentials of CeO 2−x (related to its surface state) and H 2 O 2 whose reactivity increases in the acidic regime, ii) the pK a values (determining the charge) of PR, and iii) protonation and stability of oxidized bromine species.…”
Section: Doi: 101002/adma201603823mentioning
confidence: 99%
“…Ингибитор активности МПО, ABAH снижал до фоновых значений интенсивность флуоресценции при стимуляции ней-трофилов PMA. Таким образом, был сделан вывод, согласующийся с ранее опубликованными данными, о том, что окисление CB стимулированными нейтрофи-лами отражает продукцию ими HOCl, обусловленную активностью МПО [8].…”
Section: результаты и их обсуждениеunclassified
“…Ранее для скрининга ингибиторов активности МПО нами был предложен кинетический спектрофотометрический метод изме-рения продукции HOCl по окислению красителя це-лестинового синего B -C.I. 51050 (CB) в присутствии таурина [8]. Было доказано, что CB избирательно реа-гирует на действие HOCl, окисляющей его до гликоля с переходом синей окраски в розовую.…”
Section: Introductionunclassified
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