Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase

Jewess, P. J.; Devonshire, A. L.

doi:10.1006/abio.1999.4168

Cited by 14 publications

(19 citation statements)

References 25 publications

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“…This change was not found in the midgut of the third-instar larvae of the A. aegypti not exposed to squamocin [24]. However, similar changes were observed in the A. aegypti larvae exposed to crude extracts of A. coriacea [10] and Derris urucu (Leguminosae) [25], of which the later has rotenone with action similar to that described for acetogenins [26]. …”

Section: Resultsmentioning

confidence: 52%

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Costa

Cossolin

Pereira

et al. 2014

Toxins

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Acetogenins are secondary metabolites exclusively produced by Annonaceae, which have antitumor, cytotoxic, and pesticide activities. In this study, we evaluated the larvicidal and cytotoxic effect of squamocin from Annona squamosa on Aedes aegypti (Diptera: Culicidae) midgut. The compound was solubilized in 2% Tween 20 at 10, 20, 50, 80 and 100 ppm. The assay was conducted in a completely randomized design with four replications, each with 20 third-instar larvae. Larval mortality was assessed every hour until total mortality, and the data were subjected to Probit analysis. Cellular damage was evaluated every 30 min in groups comprising five larvae subjected to squamocin at 50 and 100 ppm for 240 min. The total larval mortality occurred after 360 min following application of 50, 80, and 100 ppm squamocin, and 600 min after applying other concentrations with LC50 at 6.4 ppm. Both 50 and 100 ppm of squamocin showed cytotoxic activity in the midgut epithelium of A. aegypti after 240 min with 50 ppm resulting in midgut cells with light cytoplasm containing small vacuoles, whereas at 100 ppm were found cells with cytoplasm highly vacuolated, damaged apical surface and cell protrusion toward the gut lumen. In conclusion, squamocin has the potential to control A. aegypti.

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Section: Resultsmentioning

confidence: 52%

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Costa

Cossolin

Pereira

et al. 2014

Toxins

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“…Antimycin a (to inhibit Complex III) and cyanide (to inhibit cytochrome oxidase) were also added to the assay medium. Both assays were adapted for microtitre plates as described previously12 using 1.8 µg of submitochodrial protein per well. Groups of seven inhibitors plus one standard [3‐hydroxy‐2‐undecyl‐1,4‐naphthoquinone (UHNQ), series 1 ( n = 10)] for the Complex III assay and rotenone for the complex I assay were tested together in the same plate in the concentration range 1 × 10 −5 to 3.5 × 10 −10 M .…”

Section: Methodsmentioning

confidence: 99%

“…Groups of seven inhibitors plus one standard [3‐hydroxy‐2‐undecyl‐1,4‐naphthoquinone (UHNQ), series 1 ( n = 10)] for the Complex III assay and rotenone for the complex I assay were tested together in the same plate in the concentration range 1 × 10 −5 to 3.5 × 10 −10 M . The concentration of the inhibitor required to inhibit the enzymatic rate to 50% of the control rate was calculated from the enzymatic rates as reported previously12 and expressed as IC 50 [ M ] (± SEM) or as pI 50 (−log 10 (IC 50 )).…”

Section: Methodsmentioning

confidence: 99%

Mode of action and pesticidal activity of the natural product dunnione and of some analogues

Khambay

Batty

Jewess

et al. 2003

Pest Management Science

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This paper reports the investigation of the insecticidal and fungicidal activity of dunnione, a natural product obtained inadvertently as a by-product of a synthesis programme. Dunnione exhibits no insecticidal activity but has an unusually broad spectrum of antifungal activity. In vitro and in vivo (preventative) activities were comparable to those of several long-established fungicides (eg carbendazim). However, in whole-plant assays, its eradicant activity was unexpectedly low, probably due to poor dose-transfer from leaf surface to fungus. The level of residual activity appears to be influenced by the formulation. Finally, its potential as a lead structure was assessed, and several analogues synthesised which exhibited high activity in the in vitro assays. Mode-of-action studies revealed that dunnione exerts its action primarily through initiation of redox cycling. This contrasts to the activity of BTG 505, the biochemical/chemical precursor, which does not initiate redox cycling but instead exhibits both insecticidal and fungicidal activity by inhibiting mitochondrial Complex III.

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“…The discovery of several mitochondrial poisons together with insecticides and acaricides affecting mitochondrial electron transport has provided researchers with a series of biochemical tools to investigate the interaction of compounds with electron transport (Rickwood et al, 1987;Jewess and Devonshire, 1999;Gassner et al, 1997; Corresponding one-letter amino acid code trnX Transfer RNAs specifying leucine and serine, resp. L(CUN), L(UUR) and S(AGN), S(UCN), resp.…”

Section: Article In Pressmentioning

confidence: 99%

Complete maternal inheritance of bifenazate resistance in Tetranychus urticae Koch (Acari: Tetranychidae) and its implications in mode of action considerations

Leeuwen

Tirry

Nauen

2006

Insect Biochemistry and Molecular Biology

100

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Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase

Cited by 14 publications

References 25 publications

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Mode of action and pesticidal activity of the natural product dunnione and of some analogues

Complete maternal inheritance of bifenazate resistance in Tetranychus urticae Koch (Acari: Tetranychidae) and its implications in mode of action considerations

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