“…This makes the cellulose susceptible to enzymatic hydrolysis (Kaparaju & Felby, 2010;Liew, Shi, & Li, 2011;Xiao, Yin, Xia, & Ma, 2012) to yield sugars. Further, it is essential that any effective pretreatment method must be conducive to improve the formation of sugars by hydrolysis (Zhou, Zang, Gong, Wang, & Ma, 2012;Taherzadeh & Karimi, 2008;Lamsal, Yoo, Brijwani, & Alavi, 2010;Radeva, Valchev, Petrin, Valcheva, & Tsekova, 2012), to limit or prevent the degradation or loss of carbohydrates, to avoid the formation of degradation products that are inhibitory to the subsequent hydrolysis and fermentation processes (Talebnia, Karakashev, & Angelidaki, 2010;Thulluri, Goluguri, Konakalla, Shetty, & Addepally, 2013) and minimizing energy input for cost effectiveness (Arslan & Eken-Saracoglu, 2010). Pretreatment results must also be weighed against their impact on the ease of operation, cost of the downstream processes and the trade-off between several costs including operational, capital and biomass costs (Wyman, 1999;Zhu & Pan, 2010).…”