Kinetic modeling of the alkaline deproteinization of Nile-tilapia skin for the production of collagen

Abstract

A new phenomenological model, based on a second order dissolution kinetics, was developed for the alkaline removal of non-collagenous protein (NCP) from the skin of Nile tilapia (SNT). This model allows estimating the liquid concentration of NCP in terms of temperature, skin size, NaOH concentration and time. This model was fitted with 135 experiments averaging a R 2 of 0.99. The root-mean-square deviation and the mean-absolutepercentage error of the model were 0.0041 and 3.15%, respectively. The Arrhenius-activation energy was 15-122 kJ mol À1 . Multi-objective optimization led to the highest NCP extraction (NCPE) of 24.3% and to the lowest loss of collagen (LC) of 1.3%, with R 2 coefficients of 0.98 and 0.92, respectively. Ultimately, SNT deproteinized under optimal conditions was subjected to acid extraction and purification. FTIR and SEM analyses indicated that the product was a Type I collagen that could be used in the pharmaceutical industry.

Improving collagen processing towards a greener approach: current progress

“…12 Another finding indicated that removing non-collagenous protein from Nile tilapia skin was most efficient at 20 °C. 50 This study was also the first to report that skin size has the most impact on NaOH pre-treatment, with smaller skin sizes able to eliminate more noncollagenous protein due to larger surface area. Extensive studies evaluating collagen extraction from Bester sturgeon by-products with and without NaOH pre-treatment demonstrated that the alkaline method expedited the extraction process but significantly reduced yield.…”

“…12 Acetic acid concentration up to 1 mol L −1 has a direct effect on collagen recovery from the skin of small-spotted catshark (Scyliorhinus canicula). 50 However, the maximum collagen recovery from sole fish skin occurred at 0.6 mol L −1 and decreased gradually thereafter. It was suggested that the charge density of collagen is determined by the concentration of acetic acid in the extraction medium, which also influences its electrostatic interaction and structure, as well as its solubility and extractability from raw materials.…”

“…The concentration of acetic acid had a significant effect on collagen yield from grass carp ( Ctenopharyngodon idella ) skin at low extraction temperature (4 °C), with 0.5 mol L −1 attaining complete extraction 12 . Acetic acid concentration up to 1 mol L −1 has a direct effect on collagen recovery from the skin of small‐spotted catshark ( Scyliorhinus canicula ) 50 . However, the maximum collagen recovery from sole fish skin occurred at 0.6 mol L −1 and decreased gradually thereafter.…”

“…It was found that NaOH at 0.05 and 0.1 mol L −1 concentrations was able to remove non‐collagenous proteins without significant collagen loss at a temperature ranging from 4 to 20 °C for collagen extraction from grass carp ( Ctenopharyngodon idella ) skin 12 . Another finding indicated that removing non‐collagenous protein from Nile tilapia skin was most efficient at 20 °C 50 . This study was also the first to report that skin size has the most impact on NaOH pre‐treatment, with smaller skin sizes able to eliminate more non‐collagenous protein due to larger surface area.…”

Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults

“…In order to evaluate the structural characteristics, a Fourier transform infrared spectroscopy analysis was performed using a Thermo Scientific Nicolet iS50. The attenuated total reflectance technique (ATR) was used in a 4000–600 cm −1 range with a 4 cm −1 resolution [ 39 ].…”