Kinetic modelling of aldolase-catalyzed addition between dihydroxyacetone phosphate and (S)-alaninal

Neptunium(iv)-hydroxamate complexes: their speciation, and kinetics and mechanism of hydrolysis

“…solvent extraction, 34 solid-liquid adsorption 35 and crystallization 36 ) and homogeneous (e.g. enzyme catalyzed addition reactions 37 ). Here we employed gPROMs to determine the reaction kinetics of the homogeneous complexation/hydrolysis reactions in the Np(IV)-AHA system from speciation and kinetic run data.…”

C−C Bond-Forming Lyases in Organic Synthesis

“…Some of these derivatives proved good inhibitors for α- l -fucosidase and α- l -rhamnosidase. A thorough kinetic modeling of the FucA-mediated aldol addition of ( S )-Cbz-alaninal to DHAP revealed that DHAP is also degraded by type II aldolases and this reaction can be minimized by operating a 4 °C, leading to higher conversions. , …”

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

“…In pursuit of finding the most cost‐effective configuration for the lactol synthesis, especially for industrial use, it is of great importance for understanding the mechanisms and kinetics of enzyme systems in detail, which can be achieved by applying mathematical modeling techniques . Although the lack of reports on kinetic data obtained from kinetic models of DERA‐catalyzed reactions is evident, some kinetic studies of related aldolase‐based syntheses can be found . Ručigaj and coworkers (2015) have reported a very complex model for the sequential aldol reaction involving acetyloxyacetaldehyde, acetaldehyde and chloroacetaldehyde as substrates, which was catalyzed by crude DERA‐expressing culture lysate …”

“…[31][32][33] Although the lack of reports on kinetic data obtained from kinetic models of DERA-catalyzed reactions is evident, 3 some kinetic studies of related aldolase-based syntheses can be found. 3,34,35 Ručigaj and coworkers (2015) have reported a very complex model for the sequential aldol reaction involving acetyloxyacetaldehyde, acetaldehyde and chloroacetaldehyde as substrates, which was catalyzed by crude DERA-expressing culture lysate. 3 The aim of this work was to optimize the production of the statin precursor (4) from acetaldehyde (1) and chloroacetaldehyde (2) in the reaction catalyzed by DERA from Thermotoga maritima (DERA Tm ) ( Fig.…”