Kinetic Resolution of Pipecolic Acid Using Partially-Purified Lipase from Aspergillus niger

Preparation of 3-deacetyl cephalosporins by Aspergillus niger lipase

“…The estimated molecular weight from SDS-PAGE (Fig. 1) was 32,000, in accordance with the results recently reported by Ng-Youn-Chen et al (1994). Isoelectric focusing revealed a single band with an isoelectric point of 4.1.…”

Enzymatic resolution of amino acids via ester hydrolysis

“…The successful resolution of amino acid derivatives using porcine pancreatic lipase is worthy to note, because low enantioselectivities had been reported in the hydrolysis of other a-substituted carboxylic acid esters (Frilling and Sih, 1987;Dernoncour and Azerad, 1987). The lipase-catalyzed enantioselective ester hydrolysis was applied to the resolution of pipecolic acid (2-piperidinecarboxylic acid) (Ng-Youn- Chen et al, 1994). Crude lipase from A. niger was identified as the most enantioselective catalyst for the hydrolysis of racemic methyl pipecolate: E = 20 in favor of the L-enantiomer at pH 7.…”

Asymmetric syntheses of pipecolic acid and derivatives

“…Kazlauskas (Ng-Youn-Chen et al, 1994) screened various lipases for the kinetic resolution of pipecolate esters and found that partially purified ANL (lipase from Aspergillus niger, AP-6 from Amano-Enzyme-Co) was the best biocatalyst for this operation, as illustrated in Fig. 36.…”