Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis

Miao, Yuxin; Chen, Jie Yu; Jiang, Xuejian; Huang, Zhou

doi:10.1007/s12010-012-9689-5

Cited by 17 publications

(6 citation statements)

References 16 publications

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“…, it would be sugars in the whole pretreated slurry that resulted in the lower efficiency of enzymatic saccharification. Because, it has been reported that the sugar derivations from glucan and xylan dramatically decrease efficiency of enzymatic saccharification . However, pretreatment at higher temperature or with lower solid load resulted in higher efficiency of enzymatic saccharification (Figure B).…”

Section: Resultsmentioning

confidence: 97%

Production of bioethanol from rice straw by simultaneous saccharification and fermentation of whole pretreated slurry usingSaccharomyces cerevisiaeKF-7

Wang

Sun

et al. 2014

Environ. Prog. Sustainable Energy

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Rice straw is one of the most abundant lignocellulosic waste materials worldwide, and it can serve as feedstock for bioethanol production. In this study, ethanol was produced from rice straw by simultaneous saccharification and fermentation (SSF) of the whole pretreated slurry with high solid load. Pretreatment of rice straw using 0.5% (w/w) H2SO4 was performed to increase the efficiency of enzymatic saccharification. Enzymatic saccharification and SSF of the whole pretreated slurry were subsequently performed to evaluate the inhibitory effect on enzymatic saccharification and the final ethanol yield. The result showed that pretreatment was the key for achieving high ethanol yield of the whole pretreated slurry. Rice straw pretreated at 170°C with a solid load of 15% exhibited efficient enzymatic saccharification. By the result of ethanol yields, no inhibitory effect was found when the whole pretreated slurry was subjected to SSF. The ethanol yield of SSF of the whole pretreated slurry was enhanced to 77.3%, compared with the glucose recovery efficiency of enzymatic saccharification of the washed pretreated rice straw, which was 74.4%. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 582–588, 2015

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Section: Resultsmentioning

confidence: 97%

Production of bioethanol from rice straw by simultaneous saccharification and fermentation of whole pretreated slurry usingSaccharomyces cerevisiaeKF-7

Wang

Sun

et al. 2014

Environ. Prog. Sustainable Energy

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“…However, the low increase in glucose concentration and almost constant level of cellobiose after the first 6 h suggests that b-glucosidase activity was inhibited by glucose. Inhibition of cellobiohydrolases (EC 3.2.1.91) and endoglucanases (Andrić et al 2010;Miao et al 2012). To overcome this problem, enzymatic hydrolysis processes are either conducted in Tables 4 and 5 were correlated with apparent changes in the appearance of fibers contained in the poplar pulp, which was digested by the preparation NS-22086.…”

Section: Selection Of Enzyme Preparationmentioning

confidence: 99%

Production of glucose-rich enzymatic hydrolysates from cellulosic pulps

et al. 2014

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The digestibility of cellulosic pulps derived by the sulfate process was assessed using commercial multienzyme preparations. Poplar wood pulps of relatively low lignin contents (Kappa numbers of 15.4-24.2), birch, beech and pine wood pulps (Kappa numbers of 25.8-31.4), and wheat straw pulp (Kappa number of 29.5) were efficiently hydrolyzed by a commercial preparation NS-22086 from Novozymes, containing cellulases and xylanases. At around 1.3 % (w/w) substrate concentration, yields of glucose from the poplar pulps were around 80 % on a dry weight (d.w.) basis while for the other four pulps they varied between approximately 70 % (for pine pulp) and 78 % d.w. (for beech and wheat straw pulps). At around 7.4 % (w/w) poplar pulp (Kappa number of 24.2) concentration, glucose yield was around 61 % d.w. The NS-22086 preparation almost completely saccharified fines from a paper mill (around 74 % glucose yields on a dry weight basis) while digestion of poplar chips (particle size of 1.6-2.0 mm) and wheat straw chaff (particle size up to 6 mm) yielded around 5.3 and 14 % d.w. glucose, respectively (total reducing sugars yields of around 16 and 23 % d.w., respectively). These results show that plant biomass may be efficiently converted to glucose-rich hydrolysates by a two-step processing, consisting of kraft pulping followed by treatment with endo-and exo-type cellulases and hemicellulases. Glucose-rich hydrolysates may be also obtained by enzymatic digestion of fines from paper mills.

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“…Product release from enzyme active sites is often reversible and rebinding is common in many enzyme systems1234. Liberated product(s) can effectively compete with substrate binding to enzyme active sites and inhibit enzyme cycling.…”

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confidence: 99%

Quantitative full time course analysis of nonlinear enzyme cycling kinetics

Cao

Cruz

2013

Sci Rep

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Enzyme inhibition due to the reversible binding of reaction products is common and underlies the origins of negative feedback inhibition in many metabolic and signaling pathways. Product inhibition generates non-linearity in steady-state time courses of enzyme activity, which limits the utility of well-established enzymology approaches developed under the assumption of irreversible product release. For more than a century, numerous attempts to find a mathematical solution for analysis of kinetic time courses with product inhibition have been put forth. However, no practical general method capable of extracting common enzymatic parameters from such non-linear time courses has been successfully developed. Here we present a simple and practical method of analysis capable of efficiently extracting steady-state enzyme kinetic parameters and product binding constants from non-linear kinetic time courses with product inhibition and/or substrate depletion. The method is general and applicable to all enzyme systems, independent of reaction schemes and pathways.

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Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis

Cited by 17 publications

References 16 publications

Production of bioethanol from rice straw by simultaneous saccharification and fermentation of whole pretreated slurry usingSaccharomyces cerevisiaeKF-7

Production of bioethanol from rice straw by simultaneous saccharification and fermentation of whole pretreated slurry usingSaccharomyces cerevisiaeKF-7

Production of glucose-rich enzymatic hydrolysates from cellulosic pulps

Quantitative full time course analysis of nonlinear enzyme cycling kinetics

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