Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects

Maugeais, Cyrille; Ouguerram, Khadija; Krempf, Michel; Magot, T.

doi:10.1515/cclm.1998.131

Cited by 9 publications

(7 citation statements)

References 44 publications

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“…We used single-compartment models to describe the dynamic aspects of water metabolism both in mother and its litter related by milk flow which led to a bicompartmental model (Fig 1). The SAAM II program was used to fit the model to the observed tracer data by a weighed-least-squares approach to find the best fit as previously described [18] and to determine the parameters of the model. Implicit in the use of this model is the assumption that each animal remains in steady state with respect to its total body water turnover during the time course of the study.…”

Section: Methodsmentioning

confidence: 99%

Use of water turnover method to measure mother’s milk flow in a rat model: Application to dams receiving a low protein diet during gestation and lactation

et al. 2017

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Assessment of milk production is of utmost relevance for pediatricians and scientists interested in early life nutrition. The weight-suckle-weight (WSW) method, which consists of weighing babies before and after they suckle their mother, uses the difference in body weight as an estimate of milk intake. However, this is prone to many sources of error. In the current study, we used for the first time the water turnover method and compartmental analysis with deuterated water (D2O) as a non-toxic tracer to quantify in vivo milk production in a rat model. We assessed the effect of a nutritional intervention presumed to affect milk production, a maternal dietary protein restriction during gestation and lactation, which results in the birth of pups with intrauterine growth restriction. The specific aim of this study was to determine milk production with the body water turnover method in rat dams receiving during gestation and lactation, either a control diet (NP) or an iso-caloric low-protein diet (LP). In NP dams, mass of dam’s total body water, output flow constant from dam to litter (K21) and median milk flow, calculated between days 11 to 14 after pup birth, were 282.1 g, 0.0122 h-1 and 3.30 g/h for NP dams, respectively. Maternal dietary protein restriction (-59%) during perinatal period led to a 34% reduction in milk flow (NP versus LP). With the WSW method, milk flow varied from 1.96 g/h to 2.37 g/h between days 11 to 14 for NP dams. The main advantage of the D20 method compared to the WSW method stems from its higher precision, as attested by the narrowest range of measured values of milk flow ([2.90; 3.75] and [0.98; 6.85] g/h, respectively) for NP group. This method could be suitable for testing the effectiveness of candidate galactologue molecules presumed to enhance milk production in the lactating rat model.

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Section: Methodsmentioning

confidence: 99%

Use of water turnover method to measure mother’s milk flow in a rat model: Application to dams receiving a low protein diet during gestation and lactation

et al. 2017

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“…Each animal received intravenously a primed constant tracer infusion (10 µmol kg −1 h −1 for [5,5,5‐ 2 H 3 ]leucine and 2 µmol kg −1 min −1 for [1,2 13 C] acetate) for 8 h. Venous blood samples were withdrawn in tubes containing ethylene diamine tetraacetic acid (EDTA, final concentration of 0·2‐mmol L −1 tubes (Venoject, Paris, France) and dithiobisnitrobenzoic acid as an inhibitor of the LCAT reaction (final concentration 0·2 mmol L −1 ) at baseline, every 15 min during the first hour and then hourly until the end of the infusion. Then five blood samples were collected during the 18 h after the end of the infusion in order to cumulate specific advantages of both perfusion and bolus administration for adjustment procedures [9]. Plasma was immediately separated by centrifugation for 10 min at 4 °C; sodium azide, an inhibitor of bacterial growth, and Pefabloc SC (Interchim, Montluçon, France), a protease inhibitor, were added to the blood samples.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of cholesterol ester of apolipoprotein B100‐containing lipoproteins in dogs: evidence for disregarding cholesterol ester transfer

Bailhache

Briand

Nguyen

et al. 2004

Eur J Clin Investigation

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“…23 A forcing function determined with SAAMII program through interpolation between experimental data and corresponding to the time course of plasma leucine enrichment was used to drive the appearance of leucine tracer into apoB100 of the different lipoprotein fractions. 24 For comparison between the 3 groups (PCSK9-mutated patients, heterozygous FH, and controls) the VLDL1a, VLDL2, and VLDL1R data were presented as VLDL delipidation rate and VLDL fractional catabolic rate (FCR), which represents the sum of delipidation and direct removal rate. The VLDL conversion rate was calculated as VLDL2 delipidation flux divided by total VLDL mass.…”

Section: Modelingmentioning

confidence: 99%

Apolipoprotein B100 Metabolism in Autosomal-Dominant Hypercholesterolemia Related to Mutations inPCSK9

Ouguerram

Chétiveaux

Zaïr

et al. 2004

ATVB

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171

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Objective-We have reported further heterogeneity in familial autosomal-dominant hypercholesterolemia (FH) related to mutation in proprotein convertase subtilisin/kexin type 9 (PCSK9) gene previously named neural apoptosis regulated convertase 1 (Narc-1). Our aim was to define the metabolic bases of this new form of hypercholesterolemia. Methods and Results-In vivo kinetics of apolipoprotein B100-containing lipoproteins using a 14-hour primed constant infusion of [ 2 H 3 ] leucine was conducted in 2 subjects carrying the mutation S127R in PCSK9, controls subjects, and FH subjects with known mutations on the low-density lipoprotein (LDL) receptor gene (LDL-R). Apo B100 production, catabolism, and transfer rates were estimated from very LDL (VLDL), intermediate-density lipoprotein (IDL), and LDL tracer enrichments by compartmental analysis. PCSK9 mutation dramatically increased the production rate of apolipoprotein B100 (3-fold) compared with controls or LDL-R mutated subjects, related to direct overproduction of VLDL (3-fold), IDL (3-fold), and LDL (5-fold). The 2 subjects also showed a decrease in VLDL and IDL conversion (10% to 30% of the controls). LDL fractional catabolic rate was slightly decreased (by 30%) compared with controls but still higher than LDL-R-mutated subjects. Conclusion-These results showed that the effect of the S127R mutation of PCSK9 on plasma cholesterol homeostasis is mainly related to an overproduction of apolipoprotein B100.

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Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects

Cited by 9 publications

References 44 publications

Use of water turnover method to measure mother’s milk flow in a rat model: Application to dams receiving a low protein diet during gestation and lactation

Use of water turnover method to measure mother’s milk flow in a rat model: Application to dams receiving a low protein diet during gestation and lactation

Metabolism of cholesterol ester of apolipoprotein B100‐containing lipoproteins in dogs: evidence for disregarding cholesterol ester transfer

Apolipoprotein B100 Metabolism in Autosomal-Dominant Hypercholesterolemia Related to Mutations inPCSK9

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