Kinetic study of butanol production from mixtures of glucose and xylose and investigation of different pre-growth strategies

Abstract

This study proposes a dynamic model that describes key characteristics of fermentative butanol production from glucose and xylose mixtures. The model has 12 parameters and incorporates noncompetitive inhibitory interaction between sugars as well as inhibitions due to high substrate and butanol concentrations. Different pre-growth strategies to achieve co-utilization of sugars were explored together with their effects on fermentation kinetics. Mixed sugar fermentation by the cultures pre-grown on a mixture of glucose and xylose showed a higher endurance to inhibition, a 2-fold increase in butanol production and a 1.5-fold increase in total sugar consumption compared to cultures pre-grown on xylose only. The average squared correlation coefficients (r 2 ) between experimental observations and model predictions were 0.917 and 0.926 for fermentations done by the cultures pre-grown on xylose only, and pregrown on a mixture of glucose and xylose, respectively. Sensitivity analysis on the model parameters revealed that the growth parameters were the most critical. The proposed model can serve as a basis for modeling of microbial butanol production from lignocellulosic biomass and be applied to other substrates and microorganisms.

Butanol production from lignocellulosic sugars by Clostridium beijerinckii in microbioreactors

“…CCR reduces or prevents the utilization of C5 sugars in the presence of a preferred carbon source such as the C6 sugar glucose [ 11 ]. In our previous work, we investigated the effects of CCR on the utilization of lignocellulosic sugars in mixed form [ 12 ], modeled the cell growth on mixed sugars in a follow-up study [ 13 ] and in a later study, extended the model with sugar consumption and butanol production [ 14 ]. In the present study, we performed lignocellulosic sugar fermentations in microbioreactors and compared them to similar cultures in serum flasks for validating our previous findings, as an important step in paving the way for a more efficient, systematic bioprocess development in second generation butanol production.…”

Butanol Production from Lignocellulosic Sugars by Clostridium beijerinckii in Microbioreactors

“…CCR reduces or prevents the utilization of C5 sugars in the presence of a preferred carbon source such as the C6 sugar glucose (4). In our previous work, we investigated the effects of CCR on the utilization of lignocellulosic sugars in mixed form (5), modeled the cell growth on mixed sugars in a follow up study (6) and in a later study, extended the model with sugar consumption and butanol production (7). In the present study, we performed lignocellulosic sugar fermentations in microbioreactors and compared them to similar cultures in serum flasks for validating our previous findings, as an important step in paving the way for a more efficient, systematic bioprocess development in second generation butanol production.…”