Kinetic study of CD34+ cells during peripheral blood stem cell collections

Bojko, Peter; Stellberg, W.; Küdde, C.; Scharifi, M.; Herrmann, Martin; Mayer, Susanne; Harstrick, A.; Seeber, S.

doi:10.1002/(sici)1098-1101(1999)14:1<18::aid-jca4>3.0.co;2-#

Cited by 17 publications

(20 citation statements)

References 13 publications

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“…In our study, threefold more CD34+ cells were collected than were present in blood before leukapheresis. The recruitment was limited to MNC and CD34+ cells, which were predominantly removed during the procedure, which was also observed by other authors [8,23,24,43]. Interestingly, recruitment factor for CD34+ cells in our study was significantly higher in poor mobilizers than in good mobilizers, which points to the importance of LVL in patients who mobilize low number of CD34+ cells [17].…”

Section: Bojanic 17supporting

confidence: 87%

“…The optimal study design would be to compare products collected during the same procedure at different time, which was done in this study, and differences caused by interindividual variability were excluded. Studies of kinetics of PBSC enrichment during LVL showed variable results [3,[8][9][10][19][20][21][22][23][24][25][26][27][28][29][30][31], which could be consequence of difference in volumes of processed blood. Therefore, processed blood volume in our study was standardized and strictly related to the patient's TBV.…”

Section: Discussionmentioning

confidence: 99%

“…Although it lasts longer, patients will probably tolerate an extra hour of collection easier than another procedure on consecutive days which would increase the total cost of treatment and expose them to risks of central venous line complications and additional leukaphereses [24].…”

Section: Bojanic 17mentioning

confidence: 99%

See 2 more Smart Citations

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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Section: Bojanic 17supporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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“…7,8,[15][16][17][18][19][20] Therefore, the standardization of processed blood volumes seems to be necessary to enable a better interindividual comparison. 26,27 Moreover, animal studies showed distinct changes of G-CSF blood levels and suggest the G-CSF administration 2 h before the start of LVL.…”

Section: Discussionmentioning

confidence: 99%

“…16 Recent studies with slightly different regimens in adults gave controversial results. 17,18 Moreover, recruitment of stem cells during LVL was demonstrated in older but not in younger pediatric patients. 19 Finally, a recent study showed different kinetics of PBPC collections during LVL between adults and pediatric patients without increasing time-dependent collection efficiencies for the pediatric patients.…”

mentioning

confidence: 99%

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Cassens

Momkvist

Zuehlsdorf

et al. 2001

Bone Marrow Transplant

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Summary:Although several studies have demonstrated the efficacy of large volume leukapheresis (LVL) to yield high numbers of peripheral blood progenitor cells (PBPC), the mechanisms of stem cell release into circulation and the postulated phenomenon of PBPC recruitment during apheresis have not been investigated in detail. Therefore, we analyzed the kinetics of stem cell enrichment in a total of 34 standardized LVL for patients with hematologic malignancies (lymphoma, multiple myeloma) and solid tumors (breast cancer, rhabdomyosarcoma). LVL was started 2 h after administration of G-CSF processing six times the patient's blood volume. Cells were sequentially collected into six bags and the numbers of leukocytes, mononuclear cells (MNC), CD34 ؉ cells and colony-forming cells (CFU-GM) in each collection bag were analyzed. The numbers of PBPC collected demonstrated a continuous decrease starting after an early maximum during the second processed blood volume (P ‫؍‬ 0.001). Interestingly, these kinetics of decreasing stem cell yields during LVL were similar for both entities of patients with hematologic malignancies as well as for both groups of patients with solid tumors. In summary, a recruitment phenomenon, defined as a timedependent and LVL-induced increase of PBPC, could not be demonstrated in any of the diseases investigated. Bone Marrow Transplantation (2001) 28, 13-20.

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Kinetic studies during peripheral blood stem cell collection show CD34+ cell recruitment intra‐apheresis

Knudsen

Nikolaisen

Gaarsdal

et al. 2001

J of Clinical Apheresis

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A sufficient number of CD34+ cells in the peripheral blood stem cell product is important to achieve a rapid and sustained engraftment. The purpose of the present work was to study CD34+ cell kinetics during leukapheresis. Blood samples before and after leukapheresis were analysed for CD34+ cells in 205 procedures. The number of CD34+ cells after plus the number of CD34+ cells harvested was 1.5-fold greater than the number available at the beginning of the procedure, indicating recruitment of CD34+ cells during leukapheresis. In a subgroup of 66 procedures, granulocytes and platelets were measured. In contrast to CD34+ cells, these cell fractions were not recruited to the blood stream during leukapheresis. An additional nine patients were studied with serial blood measurements during leukapheresis, showing an initial decline that was followed by an increase in CD34+ cells during leukapheresis. In conclusion, CD34+ cells are recruited to the blood during the leukapheresis procedure in contrast to granulocytes and platelets.

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Kinetic study of CD34+ cells during peripheral blood stem cell collections

Cited by 17 publications

References 13 publications

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Kinetic studies during peripheral blood stem cell collection show CD34+ cell recruitment intra‐apheresis

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