Kinetic Study of Lasiodiplodan Production by Lasiodiplodia theobromae MMPI in a Low-Shear Aerated and Agitated Bioreactor

Tabuchi, Stéphanie Caroline Tavares; Martiniano, Sabrina Evelin; Cunha, Mário Antônio Alves da; Barbosa-Dekker, Aneli M.; Dekker, Robert F. H.; Prata, A. B.

doi:10.1007/s10924-020-01857-x

Cited by 4 publications

(3 citation statements)

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“…In this context, the production of lasiodiplodan from glycerol as a carbon source by the fungal strain L. theobromae MMPI in a shake flask revealed that the peak lasiodiplodan was measured at a 72-h time course [9]. A similar pattern was observed by Crognale et al [50], who found that the highest β-glucan production by the filamentous fungus Botryosphaeria rhodina DABAC-P82 was obtained at 72 h fermentation in the shake flask.…”

Section: Discussionsupporting

confidence: 74%

“…In this study, production of lasiodiplodan occurred from the beginning of the fermentation time and continued to the highest lasiodiplodan production at 72 h fermentation, while it was followed by a reduced production of lasiodiplodan from 72 to 114 h showing a correlation between fermentation time and lasiodiplodan formation. This finding indicated that fermentation time is of high importance to attain the highest lasiodiplodan yield when the fungal cells are in the exponential growth phase [9].…”

Section: Discussionmentioning

confidence: 90%

“…Current efforts for microbial synthesis of lasiodiplodan are based on the utilization of commercial sugars, particularly glucose [4,5,[8][9][10]. These sugars are mostly obtained from unsustainable carbon sources such as food-derived carbohydrates, which strongly compete with food chains [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

et al. 2021

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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 90%