Kinetic Study of Phosphorolysis of Dextrin by Potato Phosphorylase

Sakata, Masaru; Kawai, Takashi; Kayane, Shigeto; Ooshima, Hiroshi

doi:10.1252/jcej.40.441

Cited by 4 publications

(1 citation statement)

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“…Glc-1-P is a substrate for glucan synthesis and has a number of medicinal applications especially in cardiotherapy as it is a cytostatic compound. It is also reported to be used as an antibacterial, anti-inflammatory, and antitumor agent (Shin, Shin, and Lee, 2000;Sakata et al, 2007). Glc-1-P is also a starting substrate for the chemical synthesis of glucuronic acid and trehalose production (Takahashi, Wada, and Konai, 1996).…”

Section: Industrial (Biotechnological) Applicationsmentioning

confidence: 99%

Starch phosphorylase: Role in starch metabolism and biotechnological applications

Rathore¹,

Garg²,

Garg³

et al. 2009

Critical Reviews in Biotechnology

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The alpha-glucan phosphorylases of the glycosyltransferase family are important enzymes of carbohydrate metabolism in prokaryotes and eukaryotes. The plant alpha-glucan phosphorylase, commonly called starch phosphorylase (EC 2.4.1.1), is largely known for the phosphorolytic degradation of starch. Starch phosphorylase catalyzes the reversible transfer of glucosyl units from glucose-1-phosphate to the nonreducing end of alpha-1,4-D-glucan chains with the release of phosphate. Two distinct forms of starch phosphorylase, plastidic phosphorylase and cytosolic phosphorylase, have been consistently observed in higher plants. Starch phosphorylase is industrially useful and a preferred enzyme among all glucan phosphorylases for phosphorolytic reactions for the production of glucose-1-phosphate and for the development of engineered varieties of glucans and starch. Despite several investigations, the precise functional mechanisms of its characteristic multiple forms and the structural details are still eluding us. Recent discoveries have shed some light on their physiological substrates, precise biological functions, and regulatory aspects. In this review, we have highlighted important developments in understanding the role of starch phosphorylases and their emerging applications in industry.

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Section: Industrial (Biotechnological) Applicationsmentioning

confidence: 99%