Kinetic study on ethanol production using <i>Saccharomyces cerevisiae</i> ITV‐01 yeast isolated from sugar cane molasses

Muñiz, Benigno Ortíz; Carvajal-Zarrabal, Octavio; Torrestiana-Sánchez, Beatriz; Aguilar‐Uscanga, María Guadalupe

doi:10.1002/jctb.2441

Cited by 71 publications

(39 citation statements)

References 26 publications

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“…Once hexoses are available, the strain shows a glucose preference over fructose (data not shown) also previously reported for Saccharomyces cerevisiae [29,30]. Hexose presence in the medium controls plasmatic membrane transport rate and can also affect catabolic repression [18,20]. However, these differences between the preferences of the type of sugar consumed can vary depending on several factors: strain type, initial substrate concentration, nitrogen availability, all of which are suspected to affect fructose more than glucose consumption.…”

Section: Resultsmentioning

confidence: 50%

“…S. cerevisiae ITV-01 is a wild-type strain isolated from sugarcane molasses exhibiting interesting characteristics for ethanol production, such as osmotolerance and ethanol resistance in a synthetic medium. The best conditions reported for ethanol production were pH 3.5, 150 g L -1 glucose, a temperature of 30°C, and 81 g L -1 ethanol tolerance [16][17][18]. Several factors can affect cell growth and ethanol production; carbon source, inoculum size, initial sugar concentration, pH, temperature and oxygen supply are the most important [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Use of sugarcane molasses “B” as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations

Fernández-López

Torrestiana-Sánchez

Salgado-Cervantes

et al. 2011

Bioprocess Biosyst Eng

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Molasses "B" is a rich co-product of the sugarcane process. It is obtained from the second step of crystallization and is richer in fermentable sugars (50-65%) than the final molasses, with a lower non-sugar solid content (18-33%); this co-product also contains good vitamin and mineral levels. The use of molasses "B" for ethanol production could be a good option for the sugarcane industry when cane sugar prices diminish in the market. In a complex medium like molasses, osmotolerance is a desirable characteristic for ethanol producing strains. The aim of this work was to evaluate the use of molasses "B" for ethanol production using Saccharomyces cerevisiae ITV-01 (a wild-type yeast isolated from sugarcane molasses) using different initial sugar concentrations (70-291 g L(-1)), two inoculum sizes and the addition of nutrients such as yeast extract, urea, and ammonium sulphate to the culture medium. The results obtained showed that the strain was able to grow at 291 g L(-1) total sugars in molasses "B" medium; the addition of nutrients to the culture medium did not produce a statistically significant difference. This yeast exhibits high osmotolerance in this medium, producing high ethanol yields (0.41 g g(-1)). The best conditions for ethanol production were 220 g L(-1) initial total sugars in molasses "B" medium, pH 5.5, using an inoculum size of 6 × 10(6) cell mL(-1); ethanol production was 85 g L(-1), productivity 3.8 g L(-1 )h(-1) with 90% preserved cell viability.

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Section: Resultsmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Use of sugarcane molasses “B” as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations

Fernández-López

Torrestiana-Sánchez

Salgado-Cervantes

et al. 2011

Bioprocess Biosyst Eng

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“…On the other hand, Ortiz-Muñiz et al (33) reported that a yeast extract stimulates glucose consumption because the important cofactors biotin and riboflavin are present, and high yeast extract concentrations do not affect either S. cerevisiae growth or fermentative ability.…”

Section: Resultsmentioning

confidence: 97%

Effects of oxygen and nutrients on xylitol and ethanol production in sugarcane bagasse hydrolyzates

Castañón-Rodríguez

Portilla-Arias

Aguilar-Uscanga

et al. 2015

Food Sci Biotechnol

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The influence of oxygen and nutrient supplementation on xylitol and ethanol production in a synthetic medium and sugarcane bagasse hydrolyzates using Candida tropicalis IEC5-ITV and Saccharomyces cerevisiae ITV01-RD was investigated for evaluation of bioconversion of pentoses and hexoses present in the lignocellulosic biomass. The best oxygen transfer rate (56.05 mg of O 2 /L/h) and xylitol and ethanol yields (0.67 and 0.47 g/g, respectively) were obtained in a synthetic medium. A yeast extract had a positive effect on xylitol and ethanol production (0.64 and 0.44 g/g, respectively) at a concentration of 1 g/L. C. tropicalis and S. cerevisiae exhibited not previously observed morphological changes depending on the nutrient composition. Use of sugarcane bagasse hydrolyzates requires a good supply of oxygen and addition of a yeast extract to improve xylitol and ethanol production.

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“…41,42 These nitrogen sources also enhance sugar utilization for increased ethanol yield. [42][43] Yeast extract also supplies cofactors such as biotin and riboflavin for enhancement of ethanol yield. 43 Nevertheless, the use of yeast extract adds significant expense to the medium used in our study, and further work might focus on finding lower cost substitutes that still provide the same benefits.…”

Section: Medium Compositionmentioning

confidence: 99%

Repeated batch fermentation of immobilizedE. coliexpressingVitreoscillahemoglobin for long-term use

et al. 2017

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This study describes an efficient and reusable process for ethanol production from medium containing whey powder, using alginate immobilized ethanologenic E. coli strains either expressing (TS3) or not expressing (FBR5) Vitreoscilla hemoglobin. Reuseabilities of the FBR5 and TS3 strains were investigated regarding their ethanol production capacities over the course of 15 successive 96-h batch fermentations. The ethanol production was fairly stable over the entire duration of the experiment, with strain TS3 maintaining a substantial advantage over strain FBR5. Storage of both strains in 2 different solutions for up to 60 d resulted in only a modest loss of ethanol production, with strain TS3 consistently outperforming strain FBR5 by a substantial amount. Strains stored for 15 or 30 d maintained their abilities to produce ethanol without dimunition over the course of 8 successive batch fermentations; again strain TS3 maintained a substantial advantage over strain FBR5 throughout the entire experiment. Thus, immobilization is a useful strategy to maintain the advantage in ethanol productivity afforded by expression of Vitreoscilla hemoglobin over long periods of time and large numbers of repeated batch fermentations, including, as in this case, using media with food processing wastes as the carbon source.

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Kinetic study on ethanol production using Saccharomyces cerevisiae ITV‐01 yeast isolated from sugar cane molasses

Cited by 71 publications

References 26 publications

Use of sugarcane molasses “B” as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations

Use of sugarcane molasses “B” as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations

Effects of oxygen and nutrients on xylitol and ethanol production in sugarcane bagasse hydrolyzates

Repeated batch fermentation of immobilizedE. coliexpressingVitreoscillahemoglobin for long-term use

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