This study was conducted to determine and compare the fascicle behaviour during forward lunge exercise with different loadings lifted. Thirty recreationally active, untrained men (mean age = 21 ± 0.83 years old) were recruited and were assigned to perform forward lunge with 30% 1RM (30FL) and 70% 1RM (70FL) with both their dominant and non-dominant leg. For both dominant and non-dominant leg, results showed that FLmax, FLmin, PAmax and PAmin were all greater during 70FL compared to 30FL, p < 0.001. However, lengthening and shortening velocity were found to be greater during 30FL compared to 70FL, p < 0.05. During both 30FL and 70FL, all the fascicle behaviour variables were found to be greater in the dominant limb compared to non-dominant limb. In conclusion, fascicles were shown to response differently across different loadings executed that might affect the muscle architecture adaptations.