Kinetics and concentration effects of TPA-induced differentiation of cultured human neuroblastoma cells

Påhlman, Sven; Ruusala, Aino-Inkeri; Abrahamsson, L; Odelstad, Lena; Nilsson, Kenneth

doi:10.1016/0045-6039(83)90006-4

Cited by 106 publications

(42 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were harvested at indicated times and prepared for ODC assay as previously described by Mattsson et al (16). Protein was determined according to a modified Lowry procedure (24).…”

Section: Odc Assaymentioning

confidence: 99%

See 1 more Smart Citation

Mitogenic response of human SH-SY5Y neuroblastoma cells to insulin-like growth factor I and II is dependent on the stage of differentiation.

Mattsson¹,

Enberg²,

Ruusala³

et al. 1986

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Human insulin-like growth factor I and II (IGF-I and IGF-II) in concentrations of 1-30 ng/ml, were shown to stimulate ornithine decarboxylase activity and [3H]thymidine incorporation in human SH-SY5Y neuroblastoma cells. Proliferation of these cells was also stimulated by IGF-I and II when added to RPMI 1640 medium, fortified with selenium, hydrocortisone, transferrin, and fl-estradiol. Labeled IGF-I and II bound to SH-SY5Y cells. The cross-reaction pattern of IGF-I, IGF-II, and insulin in competing with the binding of labeled IGF-I and IGF-II, respectively, indicated that SH-SY5Y cells express both type I and type II IGF receptors. Treatment of SH-SY5Y cells for 4 d with 12-O-tetradecanoylphorbol-13-acetate (TPA), which resulted in morphological and functional differentiation and growth inhibition, abolished the mitogenic response to both IGF-I and II. Concomitantly, the binding of IGF-II disappeared almost totally, which offers a possible explanation for the reduced biological response to IGF-II after TPA treatment. In contrast, the IGF-I binding in TPA-treated cells was only reduced to ~70% of the binding to control cells. It is therefore not excluded that the IGF-I receptor could be uncoupled by TPA, with persistent binding capacity for IGF-I.

show abstract

“…Cells were harvested at indicated times and prepared for ODC assay as previously described by Mattsson et al (16). Protein was determined according to a modified Lowry procedure (24).…”

Section: Odc Assaymentioning

confidence: 99%

“…T HE human adrenergic neuroblastoma cells, SH-SY5Y, differentiate when exposed to biologically active phorbolesters (22, 24,30) or retinoic acid (23). At nanomolar concentrations, the most potent phorbol ester, 12-0-tetradecanoylphorbol-13-acetate (TPA),~ induces an increase in the concentrations of noradrenalin and neuron-specific enolase (22).…”

mentioning

confidence: 99%

Mitogenic response of human SH-SY5Y neuroblastoma cells to insulin-like growth factor I and II is dependent on the stage of differentiation.

Mattsson¹,

Enberg²,

Ruusala³

et al. 1986

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…Differentiation of SM-SYTY cells induced by TPA is almost complete [ 151, involving down-regulation of the expression of c-n?yc and increase of the expression of c-fos [20], followed by appearance of voltage-dependent Ca? '-channels [21], neuron-specific enolase [18,21], norepinephrine [22], and neurites [IS], containing stable cytoskeleton [23].…”

Section: Introductionmentioning

confidence: 99%

Intracellular delivery of protein kinase C‐α or ε isoform‐specific antibodies promotes acquisition of a morphologically differentiated phenotype in neuroblastoma cells

1992

View full text Add to dashboard Cite

The protein kinasc C (PKC) family participates in a ubiquitous ccl1 signallinS system utilizing increased turnover of' phosphoinositides. Because down-regulation or total PKC activity has been implicated in the acquisition of a morphologically differentiated phenotype in SH-SYSY ncuroblastoma cells, we aimed to identify the specific PKC isoforms in this process. Here we report that intracellular delivery of PKC-a and -e, but not -p,, -y or -S ieolbrm-specific antibodies is sufficient to induce acquisition of a morphologically differentiated phenotype in SH-SYTY ncuroblastoma ceils.

show abstract

“…Furthermore, the SH-SYSY cells express muscarinic acetylcholine receptors [l 1,121, and when the TPA-treated cells are exposed to acetylcholine, the stored noradrenaline is released via a Ca2+ -dependent process [l 11. In the present paper we demonstrate that TPA treatment of these cells [9]. Essentially, lo6 cells were seeded per 8.5 cm dish with or without differentiation inducer, and the cells were harvested at the indicated time points.…”

Section: Introductionmentioning

confidence: 54%

“…A marked increase was observed for secretogranin II already after 4 days. At 8 days the level was even higher and then declined, which in part might be due to an increase in the population of nonresponding cells [9]. On the other hand, chromogranin A levels were reduced when compared to controls.…”

Section: Resultsmentioning

confidence: 90%

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

et al. 1990

Self Cite

View full text Add to dashboard Cite

Human neuroblastoma cells were cultured either in the absence or presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) known to induce neuronal differentiation. This treatment led to a marked increase in the concentration of secretogranin II but to a decrease of chromogranin A. Analogous changes were observed for the respective mRNAs. Thus during differentiation of these cells the biosynthesis of two vesicle constituents of large dense core vesicles is differentially regulated as determined both at the mRNA and the protein level. Levels of both synaptin/synaptophysin and SV2 were also elevated but to a smaller degree than that of secretogranin II.

show abstract

Kinetics and concentration effects of TPA-induced differentiation of cultured human neuroblastoma cells

Cited by 106 publications

References 13 publications

Mitogenic response of human SH-SY5Y neuroblastoma cells to insulin-like growth factor I and II is dependent on the stage of differentiation.

Mitogenic response of human SH-SY5Y neuroblastoma cells to insulin-like growth factor I and II is dependent on the stage of differentiation.

Intracellular delivery of protein kinase C‐α or ε isoform‐specific antibodies promotes acquisition of a morphologically differentiated phenotype in neuroblastoma cells

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

Contact Info

Product

Resources

About