Kinetics and dose—response characteristics of adenosine 3′,5′‐monophosphate production by isolated rat adrenal cells stimulated with adrenocorticotrophic hormone

Mackie, Caroline; Richardson, M. C.; Schulster, Dennis

doi:10.1016/0014-5793(72)80312-0

Cited by 66 publications

(25 citation statements)

References 11 publications

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“…in the presence of external Ca2+(1.2mM) (Yanagibashi et al, 1978), this is identical to the data reported by other workers (Beall and Sayers, 1972;Mackie et al, 1972;Bowyer and Kitabchi, 1974;Honn and Chavin, 1977). It has been proposed that calcium may act at the sites before and beyond cyclic AMP formation as well as a direct effect of calcium on cyclic AMP formation (Bowyer and Kitabchi, 1974).…”

Section: Resultssupporting

confidence: 87%

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Calcium ion as "second messenger" in corticoidogenic action of ACTH.

Yanagibashi

1979

Endocrinol Japon

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SynopsisThe correlation between corticoidogenesis and Ca2+-influx in the cell was investigated using isolated rat or bovine adrenocortical cells.1) ACTH-induced corticoidogenesis in both rat and bovine adrenocortical cells was enhanced in parallel with increasing concentrations of external Ca2+. In the bovine cells but not in the rat cells, moreover, the marked stimulatory effect of external Ca2+ on the corticoidogenesis was observed despite the absence of ACTH.2) Ca2+-influx and corticoidogenesis always occurred unitedly.3) Verapamil markedly inhibited either corticoidogenesis or Ca2+-influx in response to ACTH. 4) The stimulatory effect of Ca2+ on corticoidogenesis was completely blocked by cycloheximide.It was therefore suggested that Ca2could regulate corticoidogenesis as a primary "second messenger" of ACTH through biosynthesis of so-called steroidogenic protein .

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Section: Resultssupporting

confidence: 87%

“…It is generally recognized by many workers that there is a definite dissociation between ACTH effects on corticoidogenesis and cyclic AMP formation in adrenal cortex (Beall and Sayers, 1972;Mackie et al, 1972;Bowyer and Kitabchi, 1974;Sharma et al, 1976;Honn and Chavin, 1977).…”

Section: Synopsismentioning

confidence: 99%

Calcium ion as "second messenger" in corticoidogenic action of ACTH.

Yanagibashi

1979

Endocrinol Japon

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“…When the sensitivity to hormonal stimulation of cyclic AMP production in intact adrenal cells (Mackie et al, 1972;Seelig & Sayers, 1973;Peytremann et al, 1973;Finn et al, 1976) is compared with that of the adenylate cyclase activity in membrane preparations from adrenal glands of the same species (Hoffmann et al, 1974;Londos & Rodbell, 1975;Dazord et al, 1975;Glossmann & Gips, 1975), the former system is commonly more sensitive. However, we have consistently found a KO.5 value in the range 0.3-1.0nM for all batches of bovine adrenal membranes.…”

Section: Adenylate Cyclasementioning

confidence: 99%

“…Investigations over the past 10 years have shown that corticotropin stimulates the activity of adenylate cyclase in the plasma membrane of adrenocortical cells, and the resultant increased concentration of cyclic AMP is considered to enhance the rate of corticosteroidogenesis (Grahame-Smith et al, 1967;Mackie et al, 1972;Seelig & Sayers, 1973).…”

mentioning

confidence: 99%

Modulation of the response of bovine adrenocortical adenylate cyclase to corticotropin

Glynn¹,

Cooper²,

Schulster³

1977

Biochemical Journal

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An assessment was made of some of the basic parameters responsible for the modulation of adenylate cyclase activity in a bovine adrenocortical plasma-membrane preparation. When determined at 0.1 mM-ATP, basal adenylate cyclase activity increased with increasing MgCl2 concentrations, whereas in the presence of corticotropin activity was essentially maximal at IOmM-MgCI2; high concentrations (25mM) of MgCl2 inhibited adenylate cyclase activity determined in the presence of both corticotropin and GTP. At all MgCl2 concentrations, corticotropin and GTP activated the enzyme in a synergistic fashion. The magnitude of the stimulation of basal activity produced by corticotropin was a function of Mg2+ concentration, whereas that produced by GTP appeared largely independent of Mg2+ concentration. Adenylate cyclase activity in the bovine adrenal membrane was half-maximally stimulated by corticotropin concentrations in the range 0.3-1.OnM. The concentration of corticotropin evoking half-maximum response was not significantly affected by raising the free Mg2+ concentration from 0.4 to 4.9mM, nor by the presence of GTP. In the presence of GTP, high concentrations (over 1 UM) of corticotropin inhibited adenylate cyclase activity, although no inhibition was apparent in the absence of guanine nucleotide.

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“…Such an action of cyclic GMP has been proposed to occur in the steroidogenic response of isolated adrenal cells to ACTH [9] where cyclic AMP levels and corticosterone production show a discrepancy [10,11 ] which is less marked than that observed in the testicular interstitial cell [6]. The present studies were performed to explore the possibility that a change in cyclic GMP concentration could be correlated with the action of low concentrations of gonadotropin upon steroidogenesis in isolated interstitial cells of the rat testis.…”

Section: Introductionmentioning

confidence: 98%