Kinetics and enthalpy measurements of interaction between β-amyloid and liposomes by surface plasmon resonance and isothermal titration microcalorimetry

Lin, Min-Sheng; Chiu, Hsiu-Mei; Fan, Fu Jung; Tsai, Hui Ting; Wang, Steven S.‐S.; Chang, Yung; Chen, Wen Yih

doi:10.1016/j.colsurfb.2007.03.014

Cited by 50 publications

(49 citation statements)

References 24 publications

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“…Surface plasmon pesonance (SPR) is a surface-sensitive label-free technique that offers voluble information about molecular interactions, 13,14 characterizing binding reactions, 6 measuring reaction kinetics, 15-17 thermodynamic parameters, 16 affinity constants, 18 etc with high sensitivity. Owing to these properties and abilities, SPR technology has rapidly become a standard tool within the pharmaceutical and biotechnology industries.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Sharifi¹,

Dolatabadi²,

Fathi³

et al. 2017

Bioimpacts

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Introduction: The interactions between biomacromolecules such as serum albumin (SA) and various drugs have attracted increasing research attention in recent years. However, the study of SA with those drugs that have relatively high hydrophilicity and a lower affinity for SA could be a challenging issue. At the present study, the interaction of bovine SA (BSA) with neomycin as a hydrophilic drug has been investigated using surface plasmon resonance (SPR) and molecular docking methods. Methods: BSA was immobilized on the carboxymethyl dextran hydrogel sensor chip after activation of carboxylic groups through NHS/EDC and, then, the neomycin interaction with BSA at different concentrations (1-128 µM) was investigated. Results: Dose-response sensorgrams of BSA upon increasing concentration of neomycin has been shown through SPR analysis. The small KD value (4.96 e-7 at 40°C) demonstrated high affinity of neomycin to BSA. Thermodynamic parameters were calculated through van’t Hoff equation at 4 different temperatures. The results showed that neomycin interacts with BSA via Van der Waals interactions and hydrogen bonds and increase of KD with temperature rising indicated that the binding process was entropy driven. Molecular docking study confirmed that hydrogen bond was the major intermolecular force stabilizing neomycin-BSA complex. Conclusion: The attained results showed that neomycin molecules can efficiently distribute within the body after interaction with BSA in spite of having hydrophilic properties. Besides, SPR can be considered as a useful instrument for study of the interaction of hydrophilic drugs with SA.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Sharifi¹,

Dolatabadi²,

Fathi³

et al. 2017

Bioimpacts

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“…Besides, the cytotoxicity of A␤ peptide is mediated by the interaction between peptides and the cellular membrane. And, the non-toxic A␤ may be accelerated to form fibril as cell membrane is present [10][11][12][13][14][15]. The consistent conclusion is that the cell membrane plays an important role during A␤-cell interaction.…”

Section: Effects Of Aˇconformation On Metabolic Heat and Cytotoxicitymentioning

confidence: 60%

“…Recent studies suggested that A␤ may not be toxic when deposited in amyloid plaques; instead, its neurotoxic properties might be attributed to the oligomeric or prefibrillar A␤ aggregates [11,28]. Besides, the cytotoxicity of A␤ peptide is mediated by the interaction between peptides and the cellular membrane.…”

Section: Effects Of Aˇconformation On Metabolic Heat and Cytotoxicitymentioning

confidence: 99%

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Using isothermal titration calorimetry to real-time monitor the heat of metabolism: A case study using PC12 cells and Aβ(1–40)

Wang

Lin

Chen

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…Thus, accelerated aggregation and ion channel activity with both types of lipids suggest that HMW interacts with either neutral or negatively charged liposomes with similar strength. Measured binding constants for Aβ with POPC and POPG were not statistically different (52) and this interaction was regarded to be driven by hydrophobic rather than electrostatic interactions (53). CD analysis of pre-aged Aβ samples upon interaction with POPC liposomes was also carried out (see Supplemental Information).…”

Section: Discussionmentioning

confidence: 99%

Two Disaccharides and Trimethylamine N-Oxide Affect Aβ Aggregation Differently, but All Attenuate Oligomer-Induced Membrane Permeability

et al. 2009

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Interaction between aggregates of amyloid beta protein (Aβ) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer’s disease (AD). Proposed membrane-related mechanisms of neurotoxicity include Aβ ion channel formation, membrane disruption, changes in membrane capacitance, and lipid membrane oxidation. Recently, osmolytes such as trehalose have been found to delay Aβ aggregation in vitro and reduce neurotoxicity. However, no direct measurements have separated the effects of osmolytes on Aβ aggregation versus membrane interactions. In this paper, we tested the influence of trehalose, sucrose and trimethylamine n-oxide (TMAO) on Aβ aggregation and fluorescent dye leakage induced by Aβ aggregates from liposomes. In the absence of lipid vesicles, trehalose and sucrose, but not TMAO, were found to delay Aβ aggregation. In contrast, all of the osmolytes significantly attenuated dye leakage. Dissolution of pre-formed Aβ aggregates was excluded as possible mechanism of dye leakage attenuation by measurements of Congo red binding as well as hydrogen deuterium exchange detected by mass spectrometry (HX-MS). On the other hand, the accelerated conversion of high order oligomers to fibril caused by vesicles did not take place if any of the three osmolytes presented. Instead, in the case of disaccharide, osmolytes were found to form adducts with Aβ, and change the dissociation dynamics of soluble oligomeric species. Both effects may have contributed to the observed osmolyte attenuation of dye leakage. These results suggest that disaccharides and TMAO may have very different effects on Aβ aggregation because of the different tendencies of the osmolytes to interact with the peptide backbone. However, the effects on Aβ membrane interaction may be due to much more general phenomena associated with osmolyte enhancement of Aβ oligomer stability and/or direct interaction of osmolyte with the membrane surface.

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Kinetics and enthalpy measurements of interaction between β-amyloid and liposomes by surface plasmon resonance and isothermal titration microcalorimetry

Cited by 50 publications

References 24 publications

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Surface plasmon resonance and molecular docking studies of bovine serum albumin interaction with neomycin: kinetic and thermodynamic analysis

Using isothermal titration calorimetry to real-time monitor the heat of metabolism: A case study using PC12 cells and Aβ(1–40)

Two Disaccharides and Trimethylamine N-Oxide Affect Aβ Aggregation Differently, but All Attenuate Oligomer-Induced Membrane Permeability

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