Kinetics modeling of inhibition and utilization of mixed volatile fatty acids in the formation of polyhydroxyalkanoates by Ralstonia eutropha

Yu, Jian; Si, Yingtao; Keung, Wan; Wong, Wan Keung

doi:10.1016/s0032-9592(01)00264-3

Cited by 69 publications

(43 citation statements)

References 21 publications

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“…Acids, DCW and PHAs were detected using the method of Yu et al 2002. Organic acids were detected by HPLC (Agilent 1100 equipped with a RID detector, USA) with an Aminex HPX-87 column (300 · 7.8 mm, Bio-rad, USA) at a column temperature of 60°C, and 0.0055 M H 2 SO 4 as mobile phase.…”

Section: Analytic Methodsmentioning

confidence: 99%

Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha

Yan

Sun

Ruan

et al. 2005

World J Microbiol Biotechnol

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Biosynthesis of PHAs by Raltonia eutropha during the dual nutrient-limitation-zone was investigated with mixed organic acids as carbon sources and (NH 4 ) 2 SO 4 as nitrogen source. Two different methods of maintaining the dualnutrient-limitation zone were adopted by feeding mixed acids and (NH 4 ) 2 SO 4 at determined rates into the fermentation cultures which were initially free of carbon sources (method A) or nitrogen sources (method B). The results indicate that, firstly, with the increase of the width of the dual-nutrient-limitation zone, the yield of shortchain-length-polyhydroxyalkanoates also increases and it suggests that most of the short-chain-length-polyhydroxyalkanoates were biosynthesized during the dual-nutrient-limitation zone. Secondly, in contrast with the dual-nutrient-limitation method of limiting the nitrogen source first (method B), the dual-nutrient-limitation method of limiting the carbon source first (method A) was more favourable for the production of short-chainlength-polyhydroxyalkanoates, and the maximum production of short-chain-length-polyhydroxyalkanoates of these two methods are 3.72 and 2.55 g/l, respectively.

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Section: Analytic Methodsmentioning

confidence: 99%

Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha

Yan

Sun

Ruan

et al. 2005

World J Microbiol Biotechnol

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“…Due to the inhibition of organic acids with high concentrations on cell growth [17,18], a special approach was developed to evaluate the influences of different combinations of butyrate, acetate, and lactate on cutinase production. This design method was improved based on the lattice design method.…”

Section: Effects Of Different Combinations Of Acetate Lactate and Bmentioning

confidence: 99%

Enhanced cutinase production of Thermobifida fusca by a two-stage batch and fed-batch cultivation strategy

Huo

Liu

et al. 2009

Biotechnol Bioproc E

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In this study, cutinase production by Thermobifida fusca WSH03-11 was investigated with mixed short-chain organic acids as co-carbon sources to demonstrate the possibility of producing high value-added products from organic wastes. T. fusca WSH03-11 was cultured with different combinations of butyrate, acetate, and lactate with a purpose of increasing cutinase activity. The optimum proportion of butyrate, acetate, and lactate was 4:1:3. In batch cultivation, acetate and lactate were consumed quickly, while the consumption of butyrate was depressed in the presence of acetate with a concentration higher than 0.5 g/L. Based on these results, a two-stage batch and fed-batch cultivation strategy was proposed: a batch culture with acetate and lactate as the co-carbon sources in the first 10 h, and then a fed-batch culture with a constant butyrate feeding rate of 12 mL/h during 11~20 h. By this two-stage cultivation strategy, cutinase activity, dry cell weight, and consumption rate of butyrate were increased by 70%, 103.4%, and 4.3-fold, respectively, compared to those of the batch cultivation. These results provided a novel and efficient way to produce high value-added products from organic wastes. © KSBB

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“…Among the various PHAs, poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-3-hydroxyvalerate, P (3HB-co-3HV), have attracted most attention [1][2][3][4][5]. Several studies have examined PHA production using bacteria such as Alcaligenes eutrophus [9][10][11], Ralstonia eutropha [6][7][8][9][10][11][12], Azotobacter vinelandii [13][14][15][16], Methylobacterium sp. [17], Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD using swine wastewater: Effect of supplementing glucose, yeast extract, and inorganic salts

Ryu

Cho

Goodrich

et al. 2008

Biotechnol Bioproc E

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This study examined the effect of adding glucose, yeast extract, and inorganic salts to swine wastewater (SWW) in a batch culture on the production of a biodegradable plastic, polyhydroxyalkanoate (PHA). A bacterial strain, Azotobacter vinelandii UWD, was used to produce PHA without limiting the non-carbon nutrients. The addition of glucose (30 g/L) to the SWW medium increased the level of cell growth (4.4~7.0 times) and PHA production (3.8~8.5 times) depending upon the dilution of SWW. A 50% dilution of SWW was found to be optimal considering the dry cell weight (9.40 g/L), PHA content (58 wt%), and hydroxyvalerate (HV) mol fraction in the PHA (4.3 mol%). A 75% SWW medium was more advantageous for producing PHA with a higher HV fraction (7.1 mol%) at the expense of losing 22% of PHA production. The undiluted SWW medium produced less than one third of the PHA compared with the 50% SWW medium, but the HV fraction was the highest (10.8 mol%). Regarding the effect of the glucose concentration, at 20 g/L glucose, the dry cell weight and level of PHA production increased to 9.34 g/L (0.63 g PHA/g dry cell weight) and 5.90 g/L, respectively. At 50 g/L glucose, there was no significant increase in PHA production. For the glucose-supplemented (30 g/L) 50% SWW medium, the addition of a nitrogen source (1 g/L of yeast extract) did not increase the level of cell growth or PHA production because the C:N ratio (23:1) was already close to the optimal value (22:1). Better aeration increased the productivity of PHA. External nitrogen supplements (1 g/L of yeast extract) and other essential mineral salts was not necessary for bacterial growth because they were contained in the SWW. These results suggest that SWW is an excellent feedstock for producing larger amounts of the value-added material, PHA, if it is combined with carbohydrate-rich organic waste. © KSBB

show abstract

Kinetics modeling of inhibition and utilization of mixed volatile fatty acids in the formation of polyhydroxyalkanoates by Ralstonia eutropha

Cited by 69 publications

References 21 publications

Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha

Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha

Enhanced cutinase production of Thermobifida fusca by a two-stage batch and fed-batch cultivation strategy

Production of polyhydroxyalkanoates by Azotobacter vinelandii UWD using swine wastewater: Effect of supplementing glucose, yeast extract, and inorganic salts

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