Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream (Sparus aurata L.)

Bordas, M. Angeles; Balebona, M.C.; Zorrilla, Irene; Borrego, Juan J.; Morinigo, M. A.

doi:10.1128/aem.62.10.3650-3654.1996

Cited by 77 publications

(56 citation statements)

References 45 publications

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“…The use of Langmuir adsorption isotherms to calculate the maximum number of available binding sites for bacterial cells on a given matrix is well accepted (Voice and Weber 1983;McEachran and Irvin 1985;Doig et al 1988;Staddon et al 1990;Bordas et al 1996;Sanderson et al 1996). The saturation pattern is in a good agreement to Langmuir isotherms.…”

Section: Discussionmentioning

confidence: 68%

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

Nejidat¹,

Saadi²,

Ronen³

2004

J Appl Microbiol

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Aim: To investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (TBP) degrading bacterium Achromobacter piechaudii TBPZ-N61 on chalk from a contaminated aquifer. Methods and Results: Adhesion kinetics of TBPZ-N61 to grey and white chalk from a polluted fractured chalk aquifer was tested in a batch system. Both grey and white chalk contain ca 80% CaCO 3 , while grey chalk contains more organic matter (2AE4%) than the white chalk (0AE3%) and also contains Dolmite and Clinoptilolite. Adhesion of the bacterial cells to the chalk particles (<0AE2 mm) occurred rapidly (96% of the cells within 15 min). Langmuirfitted adhesion isotherms suggest that cells in the stationary phase, which are more hydrophobic, adhere to both grey and white chalk more efficiently than cells in the logarithmic growth phase. Increasing the pH (from 6AE7 to 8AE1) caused a significant reduction in cell adhesion to the chalk. Activity of attached cells was evaluated in both batch and column experiments. Logarithmic cells adhering to white and grey chalk were more active in TBP degradation than cells in suspension. In column experiments, significant TBP degradation was retained up to 30 days after a single injection of TBPZ cells. Thereafter, activity was fully recovered by amendment of yeast extract. Chalk surfaces that were incubated in situ in contaminated groundwater for 20 days still allowed the adhesion and activity of TBPZ cells. Conclusions: Taken together, our results show that bacteria adhere efficiently to specific sites on the chalk surfaces, and that sustained bacterial activity of the attached cells can be achieved by adding a carbon source such as yeast extract which also overcome toxic constituents that may occur in some chalk types. Significance and Impact of the Study: Bioremediation of TBP-contaminated chalk aquifers is made possible by the injection of bacterial cultures.

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Section: Discussionmentioning

confidence: 68%

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

Nejidat¹,

Saadi²,

Ronen³

2004

J Appl Microbiol

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“…Vibrio anguillarum is consumed orally by fish in the marine setting and is able to survive in the acidic environment of the stomach for several hours prior to intestinal relocation and faecal expulsion (Olsson et al 1998) but oral consumption of the pathogen alone rarely results in vibriosis infection (Laurencin & Germon 1987). Our current understanding of the V. anguillarum portal of entry in a host resonates around chemotactic attraction of the pathogen to mucosal surfaces (Bordas et al 1996(Bordas et al , 1998 with surface skin and fins being the immediate site of attachment (Spanggaard et al 2000) and it is generally understood that V. anguillarum is able to penetrate a host through epithelial tissue or the gastrointestinal tract via oral or anal entry (O'Toole et al 2004). Transparent zebra fish appear at present to be the most suitable fish sample for monitoring V. anguillarum invasion when the pathogen is labelled with a fluorescent marker (O'Toole et al 2004;Oyarbide et al 2013).…”

Section: Pathology and Virulencementioning

confidence: 99%

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Hickey

Lee

2017

Reviews in Aquaculture

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Vibrio (Listonella) anguillarum is a marine bacteria that is pathogenic to a number of aquatic organisms including several species which are important to the aquaculture industry. Organisms that are infected by V. anguillarum are diagnosed with vibriosis which can be lethal in a matter of days. Vibriosis can be particularly devastating to aquaculture businesses and measures of prevention or treatment are generally quite expensive. Efforts to understand and control V. anguillarum virulence have been of high‐priority among international aquatic research studies. The knowledge that has accumulated as a result of this collective research effort is reviewed in this article.

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“…Mucus was isolated from the skin and the intestine of healthy sole and gilthead sea bream. The skin mucus was collected from the body surface according to the method described by Bordas, Balebona, Zorrilla, Borrego & Moriñigo (1996), by scraping with a rubber spatula and then dilution in sterile sea water. Gastrointestinal mucus was prepared by a modification of the method of Olsson, Jöborn, Westerdhal, Blomberg, Kjelleberg & Conway (1996).…”

Section: Isolation Of Crude Mucusmentioning

confidence: 99%

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

Chabrillón

Rico

Balebona

et al. 2005

Journal of Fish Diseases

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104

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Most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. However, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. Nineteen isolates obtained from farmed Senegalese sole, Solea senegalensis Kaup, and gilthead sea bream, Sparus aurata L., have been evaluated for their capacity to adhere to skin and intestinal mucus of Senegalese sole, and their antagonistic effect against Photobacterium damselae subsp. piscicida, an important pathogen for farmed sole. The isolates from gilthead sea bream showed the highest percentage of adhesion to sole mucus, whilst the pathogenic microorganisms assayed and the isolates from sole showed, in general, a lower ability to adhere to sole mucus. The results suggest that the adhesion to fish mucus was more dependent on the isolate tested than on the host mucus. The isolates from gilthead sea bream also showed a higher antagonistic activity against P. damselae subsp. piscicida than those from Senegalese sole. Four isolates were selected, on the basis of their adhesive ability and antagonistic effect on P. damselae subsp. piscicida, to study their interactions with the pathogen in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions. The results obtained show the ability of three isolates to reduce the adhesion of P. damselae subsp. piscicida to sole mucus under displacement and competition conditions. The adhesion of the pathogen to sole intestinal mucus was also significantly reduced when three isolates were assayed under exclusion conditions.

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Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream (Sparus aurata L.)

Cited by 77 publications

References 45 publications

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida

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