Kinetics of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle. Effect of agents possessing chaperone-like activity

Maloletkina, Olga I.; Markossian, Kira A.; Chebotareva, Natalia A.; Asryants, R.A.; Kleymenov, Sergey Y.; Poliansky, Nikolay B.; Muranov, Konstantin O.; Makeeva, Valentina F.; Bi, Kurganov

doi:10.1016/j.bpc.2012.02.001

Cited by 31 publications

(29 citation statements)

References 55 publications

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“…The position of the maximum of tryptophan fluorescence remained unchanged when the irradiation dose increased (λ max = 335 nm). The same situation was observed by us earlier upon irradiation of β L -crystallin [12] and glyceraldehyde-3-phosphate dehydrogenase [64].…”

Section: Tryptophan Fluorescence Spectra Of Uv-irradiated α-Crystallinsupporting

confidence: 72%

Quantification of anti-aggregation activity of UV-irradiated α-crystallin

Borzova

Markossian

Muranov

et al. 2015

International Journal of Biological Macromolecules

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Section: Tryptophan Fluorescence Spectra Of Uv-irradiated α-Crystallinsupporting

confidence: 72%

Quantification of anti-aggregation activity of UV-irradiated α-crystallin

Borzova

Markossian

Muranov

et al. 2015

International Journal of Biological Macromolecules

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“…The (V)UV photons emitted from the UV-jet are unlikely to be responsible for GAPDH inactivation. In previous studies, even 10 min of more intense UV radiation at wavelengths better suited to protein inactivation (260-280 and 310-315 nm) failed to inactivate GAPDH in solution [37,38]. It seems likely that, in addition to secondary reactions of photons and particles with cellular components in the cellular milieu (liquid chemistry), the ozone produced by the jets plays a significant role in GAPDH inactivation at this exposure time.…”

Section: Discussionmentioning

confidence: 99%

Photons and particles emitted from cold atmospheric-pressure plasma inactivate bacteria and biomolecules independently and synergistically

Lackmann

Schneider

Edengeiser

et al. 2013

J. R. Soc. Interface.

156

133

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Cold atmospheric-pressure plasmas are currently in use in medicine as surgical tools and are being evaluated for new applications, including wound treatment and cosmetic care. The disinfecting properties of plasmas are of particular interest, given the threat of antibiotic resistance to modern medicine. Plasma effluents comprise (V)UV photons and various reactive particles, such as accelerated ions and radicals, that modify biomolecules; however, a full understanding of the molecular mechanisms that underlie plasma-based disinfection has been lacking. Here, we investigate the antibacterial mechanisms of plasma, including the separate, additive and synergistic effects of plasma-generated (V)UV photons and particles at the cellular and molecular levels. Using scanning electron microscopy, we show that plasma-emitted particles cause physical damage to the cell envelope, whereas UV radiation does not. The lethal effects of the plasma effluent exceed the zone of physical damage. We demonstrate that both plasma-generated particles and (V)UV photons modify DNA nucleobases. The particles also induce breaks in the DNA backbone. The plasma effluent, and particularly the plasma-generated particles, also rapidly inactivate proteins in the cellular milieu. Thus, in addition to physical damage to the cellular envelope, modifications to DNA and proteins contribute to the bactericidal properties of cold atmospheric-pressure plasma.

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“…Glycogen phosphorylase b (Phb) and glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle were used as model proteins [15,16]. The advantage of such test systems is that they allow studying the effects of different agents directly on the aggregation stages.…”

Section: Introductionmentioning

confidence: 99%