Kinetics of BTEX biodegradation by a microbial consortium acclimatized to unleaded gasoline and bacterial strains isolated from it

Morlett-Chávez, Jesús A.; Ascacio-Martínez, Jorge A.; Rivas-Estilla, Ana María; Velázquez-Vadillo, Juan Francisco; Haskins, William E.; Barrera-Saldaña, Hugo A.; Acuña‐Askar, Karim

doi:10.1016/j.ibiod.2010.06.010

Cited by 26 publications

(13 citation statements)

References 26 publications

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“…1). Our findings are in agreement with previous studies including our prior report (Morlett-Chávez et al, 2010). Pseudomonas genus, have been found in BTEX contaminated sites 196 et al, 2007;Patrauchan et al, 2008).…”

Section: Discussionsupporting

confidence: 94%

“…Protein identification. Protein identification was run as previously described by Morlett-Chávez et al (2010) and Dalvi et al (2014).…”

Section: Differential Proteomic Analysismentioning

confidence: 99%

“…The amplicons were purified using the Wizard SV Genomic DNA Purification System kit (Promega, Madison WI, USA). Sequences and bioinformatics analysis were ran in accordance with Morlett-Chávez et al (2010).…”

Section: Differential Proteomic Analysismentioning

confidence: 99%

“…Recently, we reported that a consortium acclimatized to unleaded gasoline degraded 95% of total BTEX and Pseudomonas, Shewanella, Burkholderia, Alcanivorax, Rhodococcus and Bacillus, were identified by 16S rDNA. While, Pseudomonas putida strain, isolated from that consortium, was capable of removing 90% of total BTEX (Morlett-Chávez et al, 2010). Moreover, bacteria from the genus Rhodococcus have the ability to grow under a wide variety of xenobiotic compounds including aliphatic and aromatic hydrocarbons (Kim et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Morlett-Chávez

Ascacio-Martinez

Haskins

et al. 2017

Polish Journal of Microbiology

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Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase (tod) gene was up-regulated in P. putida strain when exposed to toluene, ethylbenzene, xylene, and BTEX. In contrast, the tod gene of P. putida F1 (ATCC 700007) was up-regulated only in the presence of toluene and BTEX. Several differences in the nucleotide and protein sequences of these two tod genes were observed. This suggests that tod up-regulation in P. putida strain may partially explain their great capacity to remove aromatic compounds, relative to P. putida F1. Therefore, new tod and P. putida strain are promising for various environmental applications.

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Section: Discussionsupporting

confidence: 94%

“…Protein identification. Protein identification was run as previously described by Morlett-Chávez et al (2010) and Dalvi et al (2014).…”

Section: Differential Proteomic Analysismentioning

confidence: 99%

Section: Differential Proteomic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Morlett-Chávez

Ascacio-Martinez

Haskins

et al. 2017

Polish Journal of Microbiology

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“…After 2 hours, for most initial concentrations that have been studied, the concentrations of toluene, ethylbenzene, and benzene reached zero and the biodegradation yield reached 100% after 2.5 hours of test. These values are more expressive than previous studies with other microorganisms., that obtained yields near 80 % with a kinetic time varying from 6 to 48 hours (Khodaei et al, 2017;Nagarajan and Loh, 2014;Drakou et al, 2015;El-Naas et al, 2014Morlett-Chávez et al, 2010Parameswapara et al, 2008).…”

Section: Resultsmentioning

confidence: 97%

KINETICS OF THE BIODEGRADATION OF MONOAROMATICS BY Pseudomonas aeruginosa

Câmara

Sousa

Neto

2019

Braz. J. Chem. Eng.

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Water contamination by monoaromatic compounds has risen throughout time, which leads to the necessity of developing new water treatment technology, capable of minimizing their negative effect on the environment. In this context, biological processes present themselves as a solution to the processes of extraction. Bioremediation makes use of microbial groups capable of using hydrocarbons as a source of carbon to perform their metabolic functions. This work evaluated the biodegradation efficiency of Pseudomonas aeruginosa strain isolated from contaminated matrices, for the substrates benzene, ethylbenzene and toluene, aiming to determine to which compound the bacteria had better adaptation. For that, bioremediation assays were performed for each of the monoaromatic compounds, in an isolated way, with the goal of obtaining experimental data and from this Monod and Andrews kinetic models were discretized and numerically developed through the Runge-Kutta method. It was possible to observe that Pseudomonas aeruginosa has a bigger affinity for ethylbenzene, while benzene generated a bigger microbian coefficient. Monod´s model was capable of predicting satisfactorily the experimental data.

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Multivariate optimization of headspace-GC for the determination of monoaromatic compounds (benzene, toluene, ethylbenzene, and xylenes) in waters and wastewaters

et al. 2014

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The objective of this study was to optimize, by employing a central composite rotatable design, and validate an analytical method to detect and quantify monoaromatic compounds (benzene, toluene, ethylbenzene, and xylenes) in waters and wastewaters by using headspace extraction followed by GC coupled with photoionization detection. The extraction parameters optimized were: salinity, sample volume, incubation time, and extraction temperature. The results revealed that the sample volume was the most significant parameter in the extraction process, whereas the salinity effect was negligible, which extends the applicability of the analytical method to waters with different salinities. Finally, the studied method was very selective and, at the optimal extraction conditions (15 mL sample volume, 15 min incubation time, and temperature of 70°C), presented excellent repeatability (<4%), linearity (R > 0.999 for each compound), and sensitivity, since very low LODs (0.13-0.48 μg/L) and LOQs (0.43-1.61 μg/L) were achieved.

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Kinetics of BTEX biodegradation by a microbial consortium acclimatized to unleaded gasoline and bacterial strains isolated from it

Cited by 26 publications

References 26 publications

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

KINETICS OF THE BIODEGRADATION OF MONOAROMATICS BY Pseudomonas aeruginosa

Multivariate optimization of headspace-GC for the determination of monoaromatic compounds (benzene, toluene, ethylbenzene, and xylenes) in waters and wastewaters

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