Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2)

Iwamura, Takeshi; Katsuki, Taketo

doi:10.1007/bf02776726

Cited by 13 publications

(8 citation statements)

References 7 publications

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“…This supports the findings of the previous study [6]. The tumor volume was positively correlated with the level of serum CA19-9, as in the experiments with SUIT-2 in our previous report [5]. …”

Section: Discussionsupporting

confidence: 82%

“…Thus, the establishment of biological models, including permanent cell lines of human pancreatic cancer, is required to clarify the biological characteristics of this form of cancer. Previously, we established a human pancreatic cancer cell line SUIT-2 [4], and reported on its characteristics [5, 6, 7, 8, 9, 10]. This report documents the establishment of a new human pancreatic cancer cell line (SUIT-4).…”

Section: Introductionmentioning

confidence: 99%

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Establishment and Characterization of a Novel Human Pancreatic Cancer Cell Line (SUIT-4) Metastasizing to Lymph Nodes and Lungs in Nude Mice

Kawano

Iwamura²,

Yamanari³

et al. 2004

Oncology

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A new tumor cell line (SUIT-4) derived from ascites of a patient with carcinoma of the pancreas has been established in tissue culture and in nude mice, and maintained for over 7 years. In tissue culture, the cells grew as a confluent monolayer with piling up of cells in some areas. The population doubling time during the exponential phase of the cell growth was 43.9 h in vitro. Chromosome count ranged from 63 to 68 with a modal number of 67. Subcutaneous injection of cultured cells into the flanks of nude mice resulted in tumor formation with a doubling time of 88.8 h. Histopathologically, xenografts in nude mice were moderately differentiated tubular adenocarcinoma, and the tumor cells showed spontaneous metastasis to the regional lymph nodes in 6 of 21 nude mice and to the lung in 4 of 21. Transmission electron microphotographs confirmed the ductal cell origin of the carcinoma and revealed that the cells had abundant mitochondria and lysosomes. SUIT-4 cells released carcinoembryonic antigen (3.08 × 10² ng/1 × 10⁶ cells/24 h) and carbohydrate antigen 19-9 (4.75 × 10⁴ U/1 × 10⁶ cells/24 h) during exponential cell growth in vitro. Reverse transcriptase-polymerase chain reaction studies revealed that SUIT-4 cells expressed matrix metalloproteinases 1, 3, 7, 10 and 14.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of a Novel Human Pancreatic Cancer Cell Line (SUIT-4) Metastasizing to Lymph Nodes and Lungs in Nude Mice

Kawano

Iwamura²,

Yamanari³

et al. 2004

Oncology

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“…The S2-013 cell line is a cloned subline of human pancreatic tumor cell line (SUIT-2), which was derived from a liver metastasis (30). Armenian Hamster mAb CT-2 against MUC1 CT was kindly provided by Dr. Sandra Gendler, Mayo Clinic, Scottsdale, AZ.…”

Section: Methodsmentioning

confidence: 99%

Nuclear Association of the Cytoplasmic Tail of MUC1 and β-Catenin

Wen

Caffrey

Wheelock

et al. 2003

Journal of Biological Chemistry

164

166

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MUC1, an integral membrane mucin associated with the metastatic phenotype, is overexpressed by most human carcinoma cells. The MUC1 cytoplasmic tail (CT) is postulated to function in morphogenetic signal transduction via interactions with Grb2/Sos, c-Src, and ␤-catenin. We investigated intracellular trafficking of the MUC1 CT, using epitope-tagged constructs that were overexpressed in human pancreatic cancer cell lines S2-013 and Panc-1. The MUC1 CT was detected at the inner cell surface, in the cytosol, and in the nucleus of cells overexpressing MUC1. Fragments of the MUC1 CT were associated with ␤-catenin in both cytoplasm and nuclei. Overexpression of MUC1 increased steady state levels of nuclear ␤-catenin but decreased nuclear levels of plakoglobin (␥-catenin). There was no detectable association between plakoglobin and the MUC1 CT. Coimmunoprecipitation experiments revealed that the cytoplasmic and nuclear association of MUC1 CT and ␤-catenin was not affected by disruption of Ca 2؉ -dependent intercellular cadherin interactions. These results demonstrate nuclear localization of fragments of MUC1 CT in association with ␤-catenin and raise the possibility that overexpression of the MUC1 CT stabilizes ␤-catenin and enhances levels of nuclear ␤-catenin during disruption of cadherin-mediated cell-cell adhesion.Human MUC1 is a large, type I transmembrane protein normally expressed on the apical surface of ductal epithelia (1). Full-length MUC1 is synthesized as a single polypeptide chain, which undergoes an early proteolytic cleavage (probably in the endoplasmic reticulum) creating two subunits that remain associated during its post-translational processing and transport to the cell surface (2). The larger of the two fragments contains most of the extracellular domain, including the signal sequence and a tandem repeat domain (3-5). The smaller subunit contains a short extracellular domain, transmembrane domain, and cytoplasmic tail (CT), 1 which are highly conserved across species (88% identity with murine transmembrane and CT sequence) (6). The function of MUC1 is partially elucidated for normal and transformed cells. The extracellular fragment plays a significant role in configuring the adhesive and antiadhesive properties of cells and is believed to contribute to the establishment of molecular structures that protect the cell surface in the relatively harsh environment encountered by different ductal epithelia (7). However, the function of the intracellular portion of the MUC1 cytoplasmic tail (CT) is not known. Indirect evidence suggests that the MUC1 CT is involved in signal transduction, as it contains potential docking sites for Grb2/Sos and ␤-catenin, and can be phosphorylated by GSK-3␤, c-Src, EGFR, and PKC-␦ (7-16). This, together with the general transmembrane structure of the MUC1 molecule, suggests a potential role in morphogenetic signaling; however, little is known about mechanisms by which the MUC1 CT functions in this capacity, and nothing has been reported with regard to the relationship between...

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“…U251 was derived from a glioblastoma multiforme [17], and KG-1-C was from a mixed glioma and has features of oligodendroglioma [18]. A human pancreas adenocarcinoma cell line SUIT-2 [19] was kindly provided by Dr. T. Iwamura, First Department of Surgery, Miyazaki Medical College, Japan. A human rectal adenocarcinoma cell line, RCM-1, was established in our laboratory [20].…”

Section: Cell Culturementioning

confidence: 99%

Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c‐met genes in human glioma cells in vitro

et al. 1995

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Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2)

Cited by 13 publications

References 7 publications

Establishment and Characterization of a Novel Human Pancreatic Cancer Cell Line (SUIT-4) Metastasizing to Lymph Nodes and Lungs in Nude Mice

Establishment and Characterization of a Novel Human Pancreatic Cancer Cell Line (SUIT-4) Metastasizing to Lymph Nodes and Lungs in Nude Mice

Nuclear Association of the Cytoplasmic Tail of MUC1 and β-Catenin

Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c‐met genes in human glioma cells in vitro

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