Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral

Chang, De Wei; Hsieh, Meng Ling; Chen, Yan Min; Lin, Tsair-Fuh; Chang, Jo‐Shu

doi:10.1016/j.jhazmat.2010.10.033

Cited by 50 publications

(22 citation statements)

References 22 publications

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“…5). Chang et al exposed ␤-cyclocitral to two cyanobacteria (M. aeruginosa PCC 7005 and 7820) and one diatom (Nitzschia palea) (30). They determined the effect of ␤-cyclocitral on cell integrity using an SEM and a flow cytometer.…”

Section: Discussionmentioning

confidence: 99%

Cyanobacterial Blue Color Formation during Lysis under Natural Conditions

Arii

Tsuji

Tomita

et al. 2015

Appl Environ Microbiol

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dCyanobacteria produce numerous volatile organic compounds (VOCs), such as ␤-cyclocitral, geosmin, and 2-methylisoborneol, which show lytic activity against cyanobacteria. Among these compounds, only ␤-cyclocitral causes a characteristic color change from green to blue (blue color formation) in the culture broth during the lysis process. In August 2008 and September 2010, the lysis of cyanobacteria involving blue color formation was observed at Lake Tsukui in northern Kanagawa Prefecture, Japan. We collected lake water containing the cyanobacteria and investigated the VOCs, such as ␤-cyclocitral, ␤-ionone, 1-propanol, 3-methyl-1-butanol, and 2-phenylethanol, as well as the number of cyanobacterial cells and their damage and pH changes. As a result, the following results were confirmed: the detection of several VOCs, including ␤-cyclocitral and its oxidation product, 2,2,6-trimethylcyclohexene-1-carboxylic acid; the identification of phycocyanin based on its visible spectrum; the lower pH (6.7 and 5.4) of the lysed samples; and characteristic morphological change in the damaged cyanobacterial cells. We also encountered the same phenomenon on 6 September 2013 in Lake Sagami in northern Kanagawa Prefecture and obtained almost the same results, such as blue color formation, decreasing pH, damaged cells, and detection of VOCs, including the oxidation products of ␤-cyclocitral. ␤-Cyclocitral derived from Microcystis has lytic activity against Microcystis itself but has stronger inhibitory activity against other cyanobacteria and algae, suggesting that the VOCs play an important role in the ecology of aquatic environments.

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Section: Discussionmentioning

confidence: 99%

Cyanobacterial Blue Color Formation during Lysis under Natural Conditions

Arii

Tsuji

Tomita

et al. 2015

Appl Environ Microbiol

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“…SYTOX Green (SG) has been used for the assessment of membrane integrity in cyanobacteria, diatoms, dinoflagellates and green algae (Sato et al 2004;Ribalet et al 2007;Timmermans et al 2007;Segovia and Berges 2009;Chang et al 2011;Peperzak and Brussaard 2011). SG is excluded by intact plasma membranes; thus, SG can penetrate cells with a damaged plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Machado

Soares

2014

J Appl Phycol

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This work explores the use of fluorescent probes to evaluate the responses of the green alga Pseudokirchneriella subcapitata to the action of three nominal concentrations of Cd(II), Cr(VI), Cu(II) and Zn(II) for a short time (6 h). The toxic effect of the metals on algal cells was monitored using the fluorochromes SYTOX Green (SG, membrane integrity), fluorescein diacetate (FDA, esterase activity) and rhodamine 123 (Rh123, mitochondrial membrane potential). The impact of metals on chlorophyll a (Chl a) autofluorescence was also evaluated. Esterase activity was the most sensitive parameter. At the concentrations studied, all metals induced the loss of esterase activity. SG could be used to effectively detect the loss of membrane integrity in algal cells exposed to 0.32 or 1.3 μmol L −1 Cu(II). Rh123 revealed a decrease in the mitochondrial membrane potential of algal cells exposed to 0.32 and 1.3 μmol L −1 Cu(II), indicating that mitochondrial activity was compromised. Chl a autofluorescence was also affected by the presence of Cr(VI) and Cu(II), suggesting perturbation of photosynthesis. In conclusion, the fluorescence-based approach was useful for detecting the disturbance of specific cellular characteristics. Fluorescent probes are a useful diagnostic tool for the assessment of the impact of toxicants on specific targets of P. subcapitata algal cells.

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“…This possibility was confirmed in P. subcapitata, because an algal population that consisted of >99 % SG-positive cells was not able to grow when incubated in a fresh culture medium for 7 days. SG has been used in the evaluation of the viability of bacteria, yeast cells (Haugland 2005), phytoplankton and green alga, using fluorescence microscopy, flow cytometry or microplate readers (Sato et al 2004;Ribalet et al 2007;Timmermans et al 2007;Segovia and Berges 2009;Rogers et al 2010;Chang et al 2011;Nagai et al 2011;Peperzak and Brussaard 2011;Machado and Soares 2012). Cell viability, determined by manual counting using fluorescence microscopy, is a labour-intensive method that is conducive to some inconsistency due to user error.…”

Section: Discussionmentioning

confidence: 99%

Quantification and viability analyses of Pseudokirchneriella subcapitata algal cells using image-based cytometry

Machado

Soares

2014

J Appl Phycol

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This work aims to evaluate the feasibility of using image-based cytometry (IBC) in the analysis of algal cell quantification and viability, using Pseudokirchneriella subcapitata as a cell model. Cell concentration was determined by IBC to be in a linear range between 1×10 5 and 8×10 6 cells mL −1. Algal viability was defined on the basis that the intact membrane of viable cells excludes the SYTOX Green (SG) probe. The disruption of membrane integrity represents irreversible damage and consequently results in cell death. Using IBC, we were able to successfully discriminate between live (SG-negative cells) and dead algal cells (heattreated at 65°C for 60 min; SG-positive cells). The observed viability of algal populations containing different proportions of killed cells was well correlated (R 2 =0.994) with the theoretical viability. The validation of the use of this technology was carried out by exposing algal cells of P. subcapitata to a copper stress test for 96 h. IBC allowed us to follow the evolution of cell concentration and the viability of copperexposed algal populations. This technology overcomes several main drawbacks usually associated with microscopy counting, such as labour-intensive experiments, tedious work and lack of the representativeness of the cell counting. In conclusion, IBC allowed a fast and automated determination of the total number of algal cells and allowed us to analyse viability. This technology can provide a useful tool for a wide variety of fields that utilise microalgae, such as the aquatic toxicology and biotechnology fields.

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Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral

Cited by 50 publications

References 22 publications

Cyanobacterial Blue Color Formation during Lysis under Natural Conditions

Cyanobacterial Blue Color Formation during Lysis under Natural Conditions

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Quantification and viability analyses of Pseudokirchneriella subcapitata algal cells using image-based cytometry

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