Kinetics of circulating progenitor cell mobilization during submaximal exercise

Niemiro, Grace M.; Parel, Justin T.; Beals, Joseph W.; Vliet, Stephan van; Paluska, Scott A.; Moore, Daniel R.; Burd, Nicholas A.; Lisio, Michael De

doi:10.1152/japplphysiol.00936.2016

Cited by 31 publications

(60 citation statements)

References 54 publications

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“…The EDTA‐anticoagulant tubes were used for flow cytometry analysis as previously described (Niemiro et al . 2017, ).…”

Section: Methodsmentioning

confidence: 99%

“…PBMCs were split into three different samples: CPC, MSC and common myeloid progenitor (CMP)/common lymphoid progenitor (CLP) samples. CPCs were quantified using previously defined protocols (Niemiro et al . 2017, ).…”

Section: Methodsmentioning

confidence: 99%

“…Common myeloid progenitors further differentiate along the myeloid lineage towards mixed granulocyte/macrophage, and more differentiated pure macrophage, or pure granulocyte colonies until ultimately forming mature myeloid cells, including inflammatory monocytes. HSPCs, along with more primitive haematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs) and mesenchymal stem/stromal cells (MSCs) (Niemiro et al . 2017) are collectively referred to as circulating progenitor cells (CPCs) (Niemiro et al .…”

Section: Introductionmentioning

confidence: 99%

“…HSPCs, along with more primitive haematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs) and mesenchymal stem/stromal cells (MSCs) (Niemiro et al . 2017) are collectively referred to as circulating progenitor cells (CPCs) (Niemiro et al . 2017, ).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the purpose of this study was to compare the inflammatory CPC response to a 6 week EET between lean and obese adults. We focused on a variety of CPC populations previously shown to be influenced by acute exercise (Niemiro et al . 2017), and also examined the expression of CCR2 and TLR4 specifically on HSPCs to determine if obesity/EET altered phenotypic markers associated with inflammatory cell production.…”

Section: Introductionmentioning

confidence: 99%

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Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

Niemiro

Allen

Mailing

et al. 2018

The Journal of Physiology

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Circulating progenitor cells (CPCs) and subpopulations are normally found in the bone marrow, but can migrate to peripheral tissues to participate in local inflammation and/or remodelling. The purpose of this study was to compare the CPC response, particularly the inflammatory-primed haematopoietic stem and progenitor (HSPC) subpopulation, to a 6 week endurance exercise training (EET) intervention between lean and obese adults. Seventeen healthy weight (age: 23.9 ± 5.4 years, body mass index (BMI): 22.0 ± 2.6 kg m ) and 10 obese (age: 29.0 ± 8.0 years, BMI: 33.1 ± 6.0 kg m ) previously sedentary adults participated in an EET. Blood was collected before and after EET for quantification of CPCs and subpopulations via flow cytometry, colony forming unit assays and plasma concentrations of C-X-C motif chemokine 12 (CXCL12), granulocyte-colony stimulating factor (G-CSF), and chemokine (C-C motif) ligand 2 (CCL2). Exercise training reduced the number of circulating HSPCs and adipose tissue-derived mesenchymal stem cells (AT-MSCs). EET increased the colony forming potential of granulocytes and macrophages irrespective of BMI. EET reduced the number of HSPCs expressing the chemokine receptor CCR2 and the pro-inflammatory marker TLR4. EET-induced changes in adipose tissue-derived MSCs and bone marrow-derived MSCs were negatively related to changes in absolute fitness. Our results indicate that EET, regardless of BMI status, decreases CPCs and subpopulations, particularly those primed for contribution to tissue inflammation.

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“…The EDTA‐anticoagulant tubes were used for flow cytometry analysis as previously described (Niemiro et al . 2017, ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

Niemiro

Allen

Mailing

et al. 2018

The Journal of Physiology

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Exercise and Cardiovascular Progenitor Cells

Landers‐Ramos

Sapp

Shill

et al. 2019

Comprehensive Physiology

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Autologous stem/progenitor cell‐based methods to restore blood flow and function to ischemic tissues are clinically appealing for the substantial proportion of the population with cardiovascular diseases. Early preclinical and case studies established the therapeutic potential of autologous cell therapies for neovascularization in ischemic tissues. However, trials over the past ∼15 years reveal the benefits of such therapies to be much smaller than originally estimated and a definitive clinical benefit is yet to be established. Recently, there has been an emphasis on improving the number and function of cells [herein generally referred to as circulating angiogenic cells (CACs)] used for autologous cell therapies. CACs include of several subsets of circulating cells, including endothelial progenitor cells, with proangiogenic potential that is largely exerted through paracrine functions. As exercise is known to improve CV outcomes such as angiogenesis and endothelial function, much attention is being given to exercise to improve the number and function of CACs. Accordingly, there is a growing body of evidence that acute, short‐term, and chronic exercise have beneficial effects on the number and function of different subsets of CACs. In particular, recent studies show that aerobic exercise training can increase the number of CACs in circulation and enhance the function of isolated CACs as assessed in ex vivo assays. This review summarizes the roles of different subsets of CACs and the effects of acute and chronic exercise on CAC number and function, with a focus on the number and paracrine function of circulating CD34+ cells, CD31+ cells, and CD62E+ cells. © 2019 American Physiological Society. Compr Physiol 9:767‐797, 2019.

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The impact of different forms of exercise on endothelial progenitor cells in healthy populations

et al. 2022

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Circulating endothelial progenitor cells (EPCs) contribute to vascular healing and neovascularisation, while exercise is an effective means to mobilise EPCs into the circulation. Objectives: to systematically examine the acute and chronic effects of different forms of exercise on circulating EPCs in healthy populations. Methods: Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines were followed. Results: thirty-one articles met the inclusion criteria including 747 participants aged 19 to 76 years. All included trials used flow cytometry for identification of circulating EPCs. Eight and five different EPC phenotypes were identified in the acute and chronic trials, respectively. In the acute trials, moderate intensity continuous (MICON), maximal, prolonged endurance, resistance and high intensity interval training (HIIT) exercise protocols were utilised. Prolonged endurance and resistance exercise had the most profound effect on circulating EPCs followed by maximal exercise. In the chronic trials, MICON exercise, HIIT, HIIT compared to MICON and MICON compared to exergame (exercise modality based on an interactive video game) were identified. MICON exercise had a positive effect on circulating EPCs in older sedentary individuals which was accompanied by improvements in endothelial function and arterial stiffness. Long-stage HIIT (4 min bouts) appears to be an effective means and superior than MICON exercise in mobilising circulating EPCs. In conclusion, both in acute and chronic trials the degree of exercise-induced EPC mobilisation depends upon the exercise regime applied. In future, more research is warranted to examine the dose–response relationship of different exercise forms on circulating EPCs using standardised methodology and EPC phenotype. Graphical abstract

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Kinetics of circulating progenitor cell mobilization during submaximal exercise

Cited by 31 publications

References 54 publications

Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

Exercise and Cardiovascular Progenitor Cells

The impact of different forms of exercise on endothelial progenitor cells in healthy populations

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