Kinetics of dimethoate biodegradation in bacterial system

Abstract

The present study is an investigation on the kinetics of dimethoate biodegradation and an estimation of residual dimethoate in bacterial culture by spectrophotometry. The methylene chloride extract of the culture medium was used for determination of dimethoate through its reaction with 1 chloro-2, 4 dinitrobenzene to produce methylamine whose absorbance at 505 nm gave an estimation of dimethoate content. The dimethoate standard curve follows Beer’s law at 505 nm with a slope of 0.0129 absorbance units per µg/mL. The regression equation relating concentration of dimethoate (x) with the absorbance is (y): y = 0.037 + 0.0129x. The amount of residual dimethoate after 7 days were 0, 4, 17, 28 and 29 µg/mL; the rate constants were 0.775, 0.305, 0.225, 0.167 and 0.127 each per day, and the efficiency of dimethoate degradation were 100%, 96%, 83%, 72% and 71%, for <em>Bacillus licheniformis, Pseudomonas aeruginosa, Aeromonas hydrophila, Proteus mirabilis </em>and <em>Bacillus pumilus</em> respectively. Dimethoate remediation could be attained through bacterial metabolism of the pesticide and colorimetric analysis might be useful in the estimation of dimethoate within a detection limit of 5-100 µg/mL

A comprehensive case review of microbial remediation of heavy metals and pesticides in Yamuna River

“…The effectiveness of dimethoate degradation was 100%, 96%, 83%, 72%, and 71% for B. licheniformis, P. aeruginosa, Aeromonas hydrophila, Proteus mirabilis, and B. pumilus, respectively. [190] Iprobenphos, Malathion Propenophos, Quinolphos Triazophos, Acetamiprid Carbaryl, Hexaconazole Carbendazim…”

Indonesian honey protein isolation Apis dorsata dorsata and Tetragonula sp. as antibacterial and antioxidant agent

“…Even so, the presence of bacteria resistant continues to increase and spread causing decreased ability of the anti-bacteria. The presence of bacteria resistant to these antibacterial agents continues to be alarming and continues to threaten public health [13,14].Therefore, the presence of antibacterial alternatives is needed, one of the research that continues to be done is the use of antimicrobial peptide that is in protein [15][16][17][18][19][20].…”