Kinetics of fertilization and development, and sex ratio of bovine embryos produced using the semen of different bulls

Alomar, Mazen; Tasiaux, Hélène; Remacle, Sophie; George, Fabienne; Paul, Delphine; Donnay, Isabelle

doi:10.1016/j.anireprosci.2007.06.009

Cited by 58 publications

(51 citation statements)

References 52 publications

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“…Although no plausible explanation was found for this, our results coincide with findings in the literature indicating a greater proportion of female embryos at early stages of development (21)(22)(23).…”

supporting

confidence: 93%

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Polisseni

Sá

Guerra

et al. 2010

Fertility and Sterility

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supporting

confidence: 93%

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Polisseni

Sá

Guerra

et al. 2010

Fertility and Sterility

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“…The fact that IGF1 was as effective at increasing embryonic competence to form a blastocyst when added between Days 4 and 8 as when added between Days 0 and 8 means that actions of IGF1 to increase blastocyst formation involve regulation of events associated with events after embryonic genome activation [31] and when the embryo is undergoing compaction [31,33], DNA methylation [34], proliferation and blastocoel formation [32,35,36]. There is evidence that IGF1 increases development to the blastocyst stage, at least in part, by regulating expression of genes involved in blastocoel formation.…”

Section: Discussionmentioning

confidence: 99%

Timing and dependence upon mitogen-activated protein kinase signaling for pro-developmental actions of insulin-like growth factor 1 on the preimplantation bovine embryo

Bonilla

Ozawa

Hansen

2011

Growth Hormone & IGF Research

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“…Os resultados da presente pesquisa estão em desacordo com os verificados por Wheeler et al (2006) O efeito do touro na proporção do sexo de embriões bovinos produzidos in vitro com sêmen convencional foi observado por Alomar et al (2007). Estes autores compararam a proporção do sexo de embriões provenientes da PIVE produzidos a partir de sêmen de quatro touros diferentes e observaram que em apenas um touro houve desvio na proporção do sexo a favor dos machos (76% de machos e 24% de fêmeas), enquanto os outros não diferiram do esperado de 50%.…”

Section: Discussionunclassified

Proporção Do Sexo Na Produção in Vitro De Embriões Bovinos

Loiola¹,

Rodrigues²,

Chalhoub³

et al. 2015

AVS

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RESUMO:Objetivou-se avaliar a proporção do sexo utilizando espermatozoides provenientes de sêmen convencional ou sexado para fêmea em um programa de produção in vitro de embriões (PIVE) bovinos. Foram utilizados oócitos de 87 doadoras Bos taurus indicus da raça Nelore, obtidos por sistema de aspiração folicular transvaginal guiado por ultrassonografia. Os oócitos selecionados foram maturados in vitro, fertilizados com espermatozoides oriundos de sêmen convencional (grupo 1 -CONV) ou sêmen sexado para fêmea (grupo 2 -SEX) e os embriões produzidos foram então cultivados in vitro por sete dias. Após o cultivo, os mesmos foram envasados e inovulados em receptoras mestiças Bos taurus taurus x Bos taurus indicus. Foram transferidos 1.319 embriões, sendo 1.081 produzidos com sêmen convencional (CONV) e 238 com sêmen sexado de fêmea (SEX). O diagnóstico precoce de gestação e a sexagem fetal foram realizados por ultrassonografia transretal. A taxa de gestação total foi 37,38% (493/1.319), sendo 37,74% (408/1.081) no grupo CONV e 35,71% (85/238) no grupo SEX. A proporção do sexo no grupo CONV foi desviada (P=0,002) a favor dos machos (57,84% de machos e 42,16% de fêmeas) e no grupo SEX ocorreu um desvio na proporção (P=0,001) a favor das fêmeas (68,24% de fêmeas e 31,76% de machos). Concluiuse que a utilização do sêmen convencional em PIVE bovinos elevou a proporção de produtos machos e apesar da utilização de sêmen sexado de fêmea ser uma alternativa para pré-selecionar o sexo dos produtos, ainda houve uma considerável taxa de machos quando se utilizou este tipo de sêmen.Palavras-chave: biotecnologia da reprodução; bovinocultura; FIV; nelore.ABSTRACT: This study aimed to evaluate the sex ratio using sperm from conventional or sexed semen to female in a program of in vitro production of embryos (PIVE) cattle. Oocytes from 87 donors were used Bos taurus indicus Nelore obtained by transvaginal follicular aspiration system guided by ultrasound. The selected oocytes were matured in vitro, fertilized with sperm derived from conventional semen (group 1 -CONV) or female sexed semen (group 2 -SEX) and the embryos were then cultured in vitro for seven days. After cultivation, they were packaged and ovulated on receiving crossbred Bos taurus x Bos taurus indicus. Transferred 1,319 embryos, with 1,081 produced with conventional semen (CONV) and 238 with female sexed semen (SEX). Early pregnancy diagnosis and fetal sexing were performed by transrectal ultrasonography. The overall pregnancy rate was 37.38% (493/1.319) and 37.74% (408/1.081) in CONV group and 35.71% (85/238) in group SEX. The sex ratio was skewed in the CONV group (P=0.002) in favor of males (57.84% male and 42.16% female) and SEX group was a shift in the proportion (P=0.001) in favor of females (68.24% female and 31.76% male). It was concluded that the use of conventional semen in cattle PIVE increased the proportion of males and products despite the use of sexed semen female be an alternative to preselect the sex of products, although there was a considerable rate of...

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Kinetics of fertilization and development, and sex ratio of bovine embryos produced using the semen of different bulls

Cited by 58 publications

References 52 publications

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis

Timing and dependence upon mitogen-activated protein kinase signaling for pro-developmental actions of insulin-like growth factor 1 on the preimplantation bovine embryo

Proporção Do Sexo Na Produção in Vitro De Embriões Bovinos

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