Kinetics of HDL-apo A-I in the WHHL rabbit, an animal model of familial hypercholesterolemia

In vivo kinetics of lipoprotein(a) in homozygous Watanabe heritable hyperlipidaemic rabbits

“…In earlier studies we demonstrated that rabbits are ideal animal models for examining LDL and HDL kinetics [ 18,193, and even human lipoproteins behaved similarly to the corresponding rabbit fractions [20]. Thus human LDL are recognized by the specific receptors from rabbits in a comparable manner as by human LDL-receptors.…”

“…[17] and as described [18,19]. After iodination, the free iodine was removed by filtration on a Sephadex G-25M column and subsequent dialysis against PBS solution with several bath changes.…”

“…The fractional catabolic rates (FCRs) were calculated from the areas under the decay curves, as previously described [ 18,191.…”

In vivo gene therapy for hyperlipidemia: phenotypic correction in Watanabe rabbits by hepatic delivery of the rabbit LDL receptor gene.

“…Watanabe rabbits exhibit lower apo AI levels than normal rabbits because of an increase in the rate of apo AI degradation and a decrease in rate of synthesis (41). The increase in apo AI levels after gene delivery in our experiment could be the result of increased apo Al synthesis.…”

Novel Molecular Imaging of Atherosclerosis With Gallium-68-Labeled Apolipoprotein A-I Mimetic Peptide and Positron Emission Tomography

“…Moreover, it is possible that 68 Ga-DOTA-FAMP functions not only in mature, strongly inflamed atherosclerotic plaque, but also in the initial phase of atherosclerosis. Because PET imaging cannot include the heart, we targeted the descending aorta (bifurcation of aorta), which showed less atherosclerosis than we previously reported, 21 and which was nicely viewed with PET ( Figure 7B, Upper panel for WHHL-MI). Conversely, both PET imaging and PPIS analysis of 68 Ga-DOTA-FAMP showed strong radioactivity in the aortas extracted from WHHL-MI rabbits with atherosclerotic lesions.…”