Kinetics of heteronuclear cross-relaxation in dipolar coupled-spin systems: Applications toin vivo measurements

Bachert, Peter

doi:10.1002/(sici)1099-0534(1997)9:4<227::aid-cmr3>3.0.co;2-0

“…Nevertheless, the capacity of 1 H MRI for tracing crucial biological molecules, the concentrations of which are much less than that of water molecules, is restricted by 1 H's high biological background [23] . Among other nuclei suitable for MRI, 19 F draws the attention of the research community because of its high MR sensitivity (comparable to that of 1 H), 100 % natural abundance, and negligible biological background [24–31] . Besides, the wide range of 19 F chemical shifts (>350 ppm) and the diversity of biocompatible fluorine‐containing agents permit multi‐spectral color‐coded 19 F MRI that is capable of tracing multiple targets [32–36] .…”

Section: Introductionmentioning

confidence: 99%

Glycan Metabolic Fluorine Labeling for In Vivo Visualization of Tumor Cells and In Situ Assessment of Glycosylation Variations

Chen,

Lin,

Fan

et al. 2023

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The abnormality in the glycosylation of surface proteins is critical for the growth and metastasis of tumors and their capacity for immunosuppression and drug resistance. This anomaly offers an entry point for real‐time analysis on glycosylation fluctuations. In this study, we report a strategy, glycan metabolic fluorine labeling (MEFLA), for selectively tagging glycans of tumor cells. As a proof of concept, we synthesized two fluorinated unnatural monosaccharides with distinctive 19F chemical shifts (Ac4ManNTfe and Ac4GalNTfa). These two probes could undergo selective uptake by tumor cells and subsequent incorporation into surface glycans. This approach enables efficient and specific 19F labeling of tumor cells, which permits in vivo tracking of tumor cells and in situ assessment of glycosylation changes by 19F MRI. The efficiency and specificity of our probes for labeling tumor cells were verified in vitro with A549 cells. The feasibility of our method was further validated with in vivo experiments on A549 tumor‐bearing mice. Moreover, the capacity of our approach for assessing glycosylation changes of tumor cells was illustrated both in vitro and in vivo. Our studies provide a promising means for visualizing tumor cells in vivo and assessing their glycosylation variations in situ through targeted multiplexed 19F MRI.

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Glycan Metabolic Fluorine Labeling for In Vivo Visualization of Tumor Cells and In Situ Assessment of Glycosylation Variations

Chen,

Lin,

Fan

et al. 2023

Angewandte Chemie

0

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The abnormality in the glycosylation of surface proteins is critical for the growth and metastasis of tumors and their capacity for immunosuppression and drug resistance. This anomaly offers an entry point for real‐time analysis on glycosylation fluctuations. In this study, we report a strategy, glycan metabolic fluorine labeling (MEFLA), for selectively tagging glycans of tumor cells. As a proof of concept, we synthesized two fluorinated unnatural monosaccharides with distinctive 19F chemical shifts (Ac4ManNTfe and Ac4GalNTfa). These two probes could undergo selective uptake by tumor cells and subsequent incorporation into surface glycans. This approach enables efficient and specific 19F labeling of tumor cells, which permits in vivo tracking of tumor cells and in situ assessment of glycosylation changes by 19F MRI. The efficiency and specificity of our probes for labeling tumor cells were verified in vitro with A549 cells. The feasibility of our method was further validated with in vivo experiments on A549 tumor‐bearing mice. Moreover, the capacity of our approach for assessing glycosylation changes of tumor cells was illustrated both in vitro and in vivo. Our studies provide a promising means for visualizing tumor cells in vivo and assessing their glycosylation variations in situ through targeted multiplexed 19F MRI.

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Clinical Spectroscopy

Semmler¹

Magnetic Resonance Tomography

0

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Kinetics of heteronuclear cross-relaxation in dipolar coupled-spin systems: Applications toin vivo measurements

Cited by 5 publications

References 2 publications

Glycan Metabolic Fluorine Labeling for In Vivo Visualization of Tumor Cells and In Situ Assessment of Glycosylation Variations

Glycan Metabolic Fluorine Labeling for In Vivo Visualization of Tumor Cells and In Situ Assessment of Glycosylation Variations

Glycan Metabolic Fluorine Labeling for In Vivo Visualization of Tumor Cells and In Situ Assessment of Glycosylation Variations

Clinical Spectroscopy

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