Kinetics of Inhibition Effect of 4-hydroxy-3-methoxybenzoic acid on Mushroom Tyrosinase

Gong, Sheng Zhao

doi:10.4028/www.scientific.net/amr.641-642.967

Cited by 7 publications

(9 citation statements)

References 8 publications

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“…The determination of the total phenolic content (TPC) was based on the method described by Ainsworth et al [88] and Bridi et al [89], with some modifications. Briefly, the mixture of 10% (v/v) Folin-Ciocalteu reagent and leaves extract dissolved in ethanol was incubated for 5 min at 25 • C. After that, 350 mM sodium carbonate was added.…”

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Phytochemical Screening and Antioxidant Activity of Seven Native Species Growing in the Forests of Southern Chilean Patagonia

et al. 2021

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The genus Nothofagus is one of the most abundant in the subantarctic Patagonian forests. Five species inhabit these ecosystems, three evergreen (Nothofagus betuloides, Nothofagus dombeyi, and Nothofagus nitida) and two deciduous (Nothofagus pumilio and Nothofagus antarctica). This is the first report on the levels of secondary metabolites and the antioxidant capacity of Patagonian tree species growing in natural environments. The aim of this work was to carry out a phytochemical screening, to determine the antioxidant capacity, the sun protection factor, and the α-glucosidase and tyrosinase inhibitory activity of foliar extracts of the five previous species. Besides, Aristotelia chilensis and Berberis microphylla, two species of Patagonian shrubs growing in the same forests, were used as reference. N. dombeyi was the Nothofagus with the best antioxidant capacity. B. microphylla differed from all studied species. Moreover, the Nothofagus was split into two groups. N. betuloides and N. dombeyi are the most similar species to A. chilensis. The α-glucosidase was completely inhibited by all studied extracts. Furthermore, N. antarctica, N.pumilio, and N. nitida inhibited about 70% of the tyrosinase activity. All the results found in this study for the species of the genus Nothofagus support further research on their potential beneficial properties for human health.

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Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Phytochemical Screening and Antioxidant Activity of Seven Native Species Growing in the Forests of Southern Chilean Patagonia

et al. 2021

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“…Standard solutions: Kojic acid, tropolone, isoferulic acid, resveratrol, and benzoic acid, all of which are described in the literature as tyrosinase inhibitors, were used as reference materials [1,[12][13][14]. Octinoxate [2] and dibutylphtalate were used as negative controls.…”

Section: Preparation Of Working and Standard Solutions For The Tyrosimentioning

confidence: 99%

Prevention of False-Positive Results: Development of an HPTLC Autographic Assay for the Detection of Natural Tyrosinase Inhibitors

Taibon

Ankli²,

Schwaiger

et al. 2015

Planta Med

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A simple and rapid high-performance thin-layer chromatography-based autographic assay was established to screen plant extracts for the presence of tyrosinase-inhibiting substances. Three mobile phases were selected for the chromatographic separation of different types of extracts. After development, the plate was sprayed with the substrate solution Levodopa followed by a solution of the enzyme tyrosinase. Several known tyrosinase inhibitors were tested simultaneously as positive controls. They were detected as white spots with white light in remission from the plate as well as with white light transmitted through the plate. Some of the investigated extracts included spots showing a different behaviour; some lipophilic substances appeared as white spots in white light remission but were black in white light transmission. This behaviour, which could lead to false-positive results, was due to poor wettability of the corresponding spots. False-positive results were eliminated by adding Triton X-100 to the Levodopa solution and drying the plate after 10 minutes incubation with a molecular sieve. Tyrosinase inhibitors can be clearly identified as white spots against a dark background in white light remission as well as in white light transmitted through the plate. The established high-performance thin-layer chromatography autographic assay was validated and can be used as a standard method for the detection of tyrosinase inhibitors in plant extracts without causing false-positive results.

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“…It is evident that tyrosinase is a key enzyme controlling the melanization process of skin, eye, inner ear and hair, as well as the enzymatic browning process in fruits and vegetables [3,4,5]. Meanwhile, the application of tyrosinase activators has attracted more and more attentions in the fields of cosmetic, food and pharmaceutical industry, primarily because of their high efficacy in mitigating hyperpigmentation [6].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid

Gong¹,

Chen²,

Li³

2016

Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

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The effect of 3-O-caffeoylquinic acid on the monophenolase and diphenolase activity was tested. The experimental results showed that the activation effected of 3-O-caffeoylquinic acid on both monophenolase and diphenolase activity .3-O-caffeoylquinic acid concentration leading 50% activity increase (AC 50 ) was 10.50mg/L for monophenolase and 1.10g/L for diphenolase. 3-O-caffeoylquinic acid can remove the lag time of monophenolase. The activation kinetics of 3-O-caffeoylquinic acid analyzed by Lineweaver-Burk plots demonstrated a competitive activation for the oxidation of L-DOPA and the activation constants (K A ) was determined to be 1.12g/L.

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Kinetics of Inhibition Effect of 4-hydroxy-3-methoxybenzoic acid on Mushroom Tyrosinase

Cited by 7 publications

References 8 publications

Phytochemical Screening and Antioxidant Activity of Seven Native Species Growing in the Forests of Southern Chilean Patagonia

Phytochemical Screening and Antioxidant Activity of Seven Native Species Growing in the Forests of Southern Chilean Patagonia

Prevention of False-Positive Results: Development of an HPTLC Autographic Assay for the Detection of Natural Tyrosinase Inhibitors

Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid

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