Kinetics of Inhibition of Xanthine Oxidase by Lycium arabicum and its Protective Effect against Oxonate- Induced Hyperuricemia and Renal Dysfunction in Mice

Boussoualim, Naouel; Trabsa, Hayat; Krache, Iman; Lekhmissi, Arrar; Abderrahmane, Baghiani

doi:10.4314/tjpr.v14i2.9

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…Both allopurinol and its active metabolite, oxypurinol (isosteres of hypoxanthine and xanthine, respectively), inhibit XO. Their competitive inhibitions thereby limiting the biosynthesis of uric acid hence promoting renal clearance of hypoxanthine and xanthine [ 21 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

Osman¹,

Sidik²,

Awal³

et al. 2016

J Intercult Ethnopharmacol

116

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Aim:This study was conducted to evaluate the in vitro anti-inflammatory activities and total phenolic content (TPC) of methanolic extracts of infloresence axes, endosperms, leaves, and pericarps of Barringtonia racemosa L.Methods:The anti-inflammatory study was conducted by assessing the potential through xanthine oxidase (XO) and albumin denaturation inhibition assays. Meanwhile, the TPC in the extracts were assessed by Folin-Ciocalteu assay.Results:In the XO inhibition assay, the infloresence axes extract was found to exert the highest inhibition capacity at 0.1% (w/v) with 59.54 ± 0.001% inhibition followed by leaves (58.82 ± 0.001%), pericarps (57.99 ± 0.003%), and endosperms (57.20 ± 0.003%) extracts. Similarly in the albumin denaturation inhibition assay, the infloresence axes extract had shown the greatest inhibition capacity with 70.58 ± 0.004% inhibition followed by endosperms (66.80 ± 0.024%), leaves (65.29 ± 0.006%), and pericarps extracts (43.33 ± 0.002%). Meanwhile, for TPC analysis, leaves extract was found to have the highest phenolic content (53.94 ± 0.000 mg gallic acid equivalent [GAE]/g DW) followed by infloresence axes (31.54 ± 0.001 mg GAE/g DW), endosperms (22.63 ± 0.001 mg GAE/g DW), and the least was found in pericarps (15.54 ± 0.001 mg GAE/g DW).Conclusion:The results indeed verified the in vitro anti-inflammatory activities of B. racemosa and supported its potential to be used in alleviating gouty arthritis and XO-related diseases.

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Section: Discussionmentioning

confidence: 99%

In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

Osman¹,

Sidik²,

Awal³

et al. 2016

J Intercult Ethnopharmacol

116

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“…Pada prinsipnya aktivitas SOD dapat diukur berdasarkan laju penghambatan reduksi ferricytochrome C oleh aniun superoksida yang dihasilkan oleh rekasi dari xantin dengan xantin oksidase [40]. Trabsa et al, (2015) juga telah mengaplikasikan BMXO sebagai kit dalam mempelajari efek hipourikemik dari ekstrak tanaman herbal untuk penanganan kondisi hiperuriskemia. Sehingga diharapkan dapat memeproleh suatu obat herbal yang mampu menghamat xantin oksidase.…”

Section: Pemanfaatan Xantin Oksidaseunclassified

“…Sehingga diharapkan dapat memeproleh suatu obat herbal yang mampu menghamat xantin oksidase. Dilaporkan bahwa ekstrak Lycium arabicum merupakan salah ekstrak tanaman yang menujukan penghambatan kuat terhadap BMXO sehingga memiliki efek penurunan yang signifikan pada kadar serum dan kreatinin hati serta urea pad tikus hiperuriskemia [41].…”

Section: Pemanfaatan Xantin Oksidaseunclassified

Review Artikel: Isolasi Dan Pengukuran Aktivitas Enzim Xantin Oksidase

Rohmat

Herdyastuti²

2021

UJC

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Abstrak. Xantin oksidase adalah enzim yang mengkatalisis oksidasi hipoxantin menjadi xantin dan kemudian xantin menjadi asam urat yang memerankan peran penting dalam katabolisme purin. Xantin oksidase dapat diisolasi dari berbagai sumber susu, jaringan hewan, dan mikroorganisme baik mesofilik maupun termofilik. Pemurnian enzim xantin oksidase untuk mendapatkan aktivitas enzim yang tinggi telah dilakukan dengan berbagai metode antara lain, presipitasi amonium sulfat, dialisis, dan berbagai macam metode kromatografi. Pengukuran aktivitas enzim xantin oksidase dapat dilakukan secara in vintro dengan mengukur kadar produk ataupun kadar substrat yang beraksi. xantin oksidae berpotensi diaplikasikan dalam bidang medis sebagai kit, bidang industri sebagai biosensor dan penyiapan makanan sehat rendah purin, serta dalam bidang lingkungan hidup sebagai bioremediasi senyawa heterogen siklik yang berbahaya. Kata kunci: Xantin oksidase, isolasi, pengukuran aktivitas, aplikasi Abstract. Xanthine oxidase is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and then xanthine to uric acid which be an important role in purine catabolism. Xanthine oxidase can be isolated from various sources of milk, animal tissue, and mesophilic and thermophilic microorganisms. Purification of xanthine oxidase to obtain high enzyme activity has been carried out by various methods include ammonium sulfate precipitation, dialysis, and various chromatographic methods. Measurement of xanthine oxidase enzyme activity can be doing by in vitro with measuring product levels or substrate levels in action. xanthine oxide has the potential to be applied in the medical field as a kit, in the industrial field as a biosensor and preparation of low purine healthy foods, as well as in the environmental field as a bioremediation of dangerous cyclic heterogeneous compounds. Keywords: Xanthine oxidase, isolation, activity measurement, application

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“…, 1992-2016PFAF, 2016;Lim, 2012;Liu, et al, 2004;Li, et al, 2001;Ali, 1964;ETHMEDmm, 2016;;Koleva, et al, 2015;Deeb, et al, 2013;MPNS,2017;Quattrocchi, 2012 Ali, 1980;Azadi, 2007;Li, et al, 2001; L. decumbens Welw. , 2016;Fratkin, 1996;Dafni & Yaniv, 1994;Said et al, 2002;El Hamrouni, 2001;Boullard, 2001;Pieroni, et al, 2002;Al-Quran, 2007;El-Mokasabi, 2014;Turker, 2012;Leporatti, et al, 2009;Licata et al, 2016 Kindscher, et al, 2012;Saunders, 1920;McClendon, 1921;Powell, 1988;Vines, 1960;Hodgson, 2001;Middleditch, 2012;MPNS,2017;Quattrocchi, 2012 Seifu, 2004;Soltan, et al, 2009;Cherouana et al, 2013;Ghazanfar, 1994;Hassan-Abdallah, et al, 2013 ;Trabsa et al, 2015;…”

Section: Botanymentioning

confidence: 99%

The genus Lycium as food and medicine: A botanical, ethnobotanical and historical review

Yao

Heinrich

Weckerle

2018

Journal of Ethnopharmacology

163

113

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Kinetics of Inhibition of Xanthine Oxidase by Lycium arabicum and its Protective Effect against Oxonate- Induced Hyperuricemia and Renal Dysfunction in Mice

Abstract: Purpose: To evaluate the in-vitro inhibition of xanthine oxidase (purified from bovine milk) by extracts of Lycium arabicum, as well as it is in vivo hypouricemic

Cited by 7 publications

References 28 publications

In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

Review Artikel: Isolasi Dan Pengukuran Aktivitas Enzim Xantin Oksidase

The genus Lycium as food and medicine: A botanical, ethnobotanical and historical review

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