Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)

Serum immunoglobulin M in Atlantic halibut (Hippoglossus hippoglossus): Characterisation of the molecule and its immunoreactivity

“…Coupled to various carriers, haptenic DNP is a widely used model antigen that has been shown to elicit antibody responses also in teleost fish [30,53,54]. The anti-DNP-HSA antibody activity observed in non-immunised fish is not surprising.…”

Immune- and enzyme histochemical characterisation of leukocyte populations within lymphoid and mucosal tissues of Atlantic halibut (Hippoglossus hippoglossus)

“…It has been shown in various situations that immersion vaccination of teleosts invokes a stronger local immune response than does intraperitoneal vaccination in the absence of powerful adjuvants [7,55,56]. Furthermore, the differences in cell populations observed between the gills and posterior intestine would argue for a compartmentalisation of the mucosal immune system in halibut.…”

B Cell Heterogeneity in the Teleost Kidney: Evidence for a Maturation Gradient from Anterior to Posterior Kidney

“…Assays were done as described previously (18,37): PVDF membranes were coated with 1-14 mAb at 1 g/ml, and remaining sites were blocked for 1 h with a solution of 3% casein and 4% sucrose. Cells in RPMI 1640 were added to wells in the range of 10 6 , 10 5 , and 10 4 , and incubated for 16 h at 18°C in the presence of blood gas.…”