Kinetics of lactate and glucose metabolism in the pregnant ewe and conceptus

Hodgson, John; Field, AC

doi:10.1113/expphysiol.1991.sp003506

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…When glucose precursor supply is limited in breeding ewes, glucose and lactate interconversions may assume a greater significance. Thus Hodgson, Mellor & Field (1991) reported that when pregnant ewes were fed at a fixed level, 13 % of glucose turnover was derived from lactate in monotocous ewes, rising to 31 % in ditocous ewes, values in the same range as the present study (18-31 %) and that of Faichney et al (1981) (22%). In comparison Baird et al (1983) fed ewes to meet requirements and reported that only % of glucose was derived from lactate in pregnancy, falling to 5 % in early lactation.…”

Section: Discussionsupporting

confidence: 83%

Glucose and L‐lactate metabolism in pregnant and in lactating ewes fed barley‐ or ground maize‐based diets

Perry¹,

Janes²,

Weekes³

et al. 1994

Experimental Physiology

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SUMMARYThe contribution of glucose absorbed from the small intestine to whole-body glucose metabolism was examined in ewes during late pregnancy and early lactation, using diets based on ground barley or ground maize. Glucose and L-lactate turnover in the whole body and the mesenteric-drained viscera were investigated in these ewes using isotope dilution techniques. The net absorption of glucose by the mesenteric-drained viscera and whole-body glucose turnover were unaffected by diet or reproductive status. Arterial and mesenteric venous blood glucose concentrations, arterial and mesenteric venous L-lactate concentrations and wholebody lactate turnover were all significantly higher in lactation than in pregnancy. The wholebody rates of lactate conversion to glucose and to other products were significantly higher in lactation than in pregnancy. Using a two-pool model of whole-body glucose and lactate metabolism, a relatively high percentage of glucose was converted to lactate (64-84%), accounting for 60-80% of whole-body lactate turnover. A model of glucose and lactate metabolism by the mesenteric-drained viscera was constructed. Both the calculated endogenous glucose production and the visceral conversion of glucose to lactate were greater in lactation than in pregnancy when barley was fed. Calculated total glucose absorption was relatively constant, contributing between 26 and 59% of whole-body glucose turnover. Diet had little effect on glucose and lactate metabolism in either the mesenteric-drained viscera or the wholebody of breeding ewes.

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Section: Discussionsupporting

confidence: 83%

Glucose and L‐lactate metabolism in pregnant and in lactating ewes fed barley‐ or ground maize‐based diets

Perry¹,

Janes²,

Weekes³

et al. 1994

Experimental Physiology

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Metabolic pathways of glucose and fructose: II Spatiotemporal expression of genes involved in synthesis and transport of lactate in ovine conceptuses

Moses,

Stenhouse,

Halloran

et al. 2024

Biology of Reproduction

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Lactate, an abundant molecule in fetal fluids and blood of mammalian species, is often overlooked as a metabolic waste product generated during pregnancy. Most of the glucose and fructose consumed by ovine conceptuses is converted to lactate, but proteins involved in lactate metabolism and transport have not been investigated. This study characterized total lactate produced by ovine conceptuses throughout gestation, as well as expression of mRNAs and proteins involved in lactate metabolism. Lactate increased in abundance in the uterine lumen during the preimplantation period and was more abundant than pyruvate. The abundance of lactate in allantoic and amniotic fluids increased with advancing days of gestation and most abundant on Day 125 of pregnancy (P < 0.05). Lactate dehydrogenase subunits A (converts pyruvate to lactate) and B (converts lactate to pyruvate) were expressed by conceptuses throughout gestation. Lactate is transported via monocarboxylic acid transporters SLC16A1 and SLC16A3, both of which were expressed by the conceptus throughout gestation. Additionally, the interplacentomal chorioallantois from Day 126 expressed SLC16A1 and SLC16A3 and transported lactate across the tissue. Hydrocarboxylic acid receptor 1 (HCAR1), a receptor for lactate, was localized to the uterine luminal and superficial glandular epithelia of pregnant ewes throughout gestation and conceptus trophectoderm during the peri-implantation period of gestation. These results provide novel insights into the spatiotemporal profiles of enzymes, transporters, and receptor for lactate by ovine conceptuses throughout pregnancy.

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Feeding corn during the last 12 days of gestation improved colostrum production and neonatal activity in goats grazing subtropical semi-arid rangeland1

Ramírez-Vera

Terrazas

Delgadillo

et al. 2012

Journal of Animal Science

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The objectives were to investigate if partial substitution of a grazing diet with a supplemental high-starch feed during the last 12 d of gestation improved colostrum yield and neonatal activity in goat does grazing semi-arid rangeland. For the first objective, 25 pregnant does were randomly assigned to 1 of 2 treatments: 1) grazing only (control; n = 11), and 2) grazing plus 0.6 kg/d of supplemental corn (as fed), a high starch feed, during the last 12 ± 1.0 d before parturition (SC; n = 14). Colostrum was collected at parturition and again at 1, 3, 6, and 10 h postpartum for yield and composition. In all goats, blood concentrations of glucose and plasma progesterone were determined. Total colostrum yield/10 h was greater (P = 0.002) in the SC does (1,102 ± 144 g) than in control does (405 ± 50 g). From 6 to 10 h postpartum, contents of colostrum protein and solids not fat were less (P ≤ 0.012) whereas lactose contents were greater (P = 0.035) in the SC does than in control does. Concentration of glucose in the blood at parturition was greater (P = 0.037) in the SC does (160 ± 13 mg/dL) than in control does (115 ± 12 mg/dL). A significant decrease (P = 0.001) in plasma progesterone concentrations occurred 1 d before parturition in control does, whereas the decrease (P = 0.008) occurred 3 d before parturition in SC does. For the second objective, 20 does (10 per group) and their single kids were randomly assigned to the same treatments as above for evaluation of dietary treatment on neonatal activity. Kid activity was assessed using videos recorded during the first 90 min after birth. Frequency of low-pitched bleats was greater (P < 0.001) in kids of SC does than in kids of controls. Frequencies and durations of teat seeking from 30 to 90 min after birth and of suckling activity were greater (P ≤ 0.015) in kids of SC does than in kids of controls. Duration of parturition was longer (P = 0.001) in control does (58 ± 10.2 min) than in SC does (21 ± 2.8 min). Finally, percentage of fetal malpresentation was greater (P = 0.036) in control does (33.3%) than in SC does (9.0%). We conclude that partial substitution of the grazing diet with a high-starch feed, corn, during the last 12 d of gestation improved colostrum yield and neonatal activity in goats grazing subtropical semi-arid rangeland.

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Kinetics of lactate and glucose metabolism in the pregnant ewe and conceptus

Cited by 4 publications

References 22 publications

Glucose and L‐lactate metabolism in pregnant and in lactating ewes fed barley‐ or ground maize‐based diets

Glucose and L‐lactate metabolism in pregnant and in lactating ewes fed barley‐ or ground maize‐based diets

Metabolic pathways of glucose and fructose: II Spatiotemporal expression of genes involved in synthesis and transport of lactate in ovine conceptuses

Feeding corn during the last 12 days of gestation improved colostrum production and neonatal activity in goats grazing subtropical semi-arid rangeland1

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