Kinetics of matrix synthesis in cartilage cell cultures*1

Lavietes, Beverly B.

doi:10.1016/0014-4827(71)90584-2

Cited by 41 publications

(16 citation statements)

References 21 publications

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“…Vitamin C increases sulfated MPS synthesis by embryonic chick chondrocytes in vitro (11)(12)(13). The present findings are a t variance with the preceding and presumably represent a n o t h e r species difference.…”

Section: Discussionsupporting

confidence: 62%

Preliminary experience with cell culture of human articular chondrocytes

Srivastava

Malemud

Hough

et al. 1974

Arthritis & Rheumatism

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Articular chondrocytes from 1 4 human amputation specimens were grown in monolayer culture. The joint cartilages appeared normal grossly. The ages of the patients ranged from 13 to 62 years. The cells grew slowly and did not thrive in subculture. Vitamin C increased DNA synthesis by the chondrocytes but decreased incorporation of radiosulfate into mucopolysaccharides. Whole articular cartilage from a 30 year old man showed a great heterogeneity of mucopolysaccharides. In addition to the expected chondroitin sulfates 4 and 6 and keratan sulfate, appreciable quantities of hyaluronate, chondroitin, disulfated chondroitin and dermatan sulfate were found (4.0%, 1.5%, 25.5% and 1.3% of the total mucopoSysaccharides respectively). The same compounds were secreted into the medium by the cultured chondrocytes but in distorted proportions. These and other findings differ greatly from those obtained with rabbit chondrocytes which grow readily under the same conditions and recapitulate quite faithfully the mucopolysaccharide synthesis occurring in whole cartilage.

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Section: Discussionsupporting

confidence: 62%

Preliminary experience with cell culture of human articular chondrocytes

Srivastava

Malemud

Hough

et al. 1974

Arthritis & Rheumatism

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“…Many investigators have reported that the addition of ascorbic acid and P-glycerophosphate to medium is required for high efficiency calcification. Ascorbic acid may promote cell proliferation (Hajek and Solursh, 1975), and the synthesis of collagen and glycosaminoglycan (Lavietes, 1971;Hajek and Solursh, 1975), whereas f3-glycerophosphate may act as a primary physicochemical factor on hydroxyapatites (Suzuki et al, 1981;Khouja et al, 19901, as an enhancer of alkaline phosphatase, at least in vitro (Maniatopoulos et al, 1988;Khouja et al, 19901, and may be associated with cell dystrophy (Gronowiz et al, 1989;Zimmermann et al, 1991). In the present culture system, matrix calcification may be accelerated more rapidly and with higher efficiency by an addition of P-glycerophosphate.…”

Section: Discussionmentioning

confidence: 86%

Mouse Meckel's cartilage chondrocytes evoke bone-like matrix and further transform into osteocyte-like cells in culture

et al. 1996

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Background We reported that when Meckel's cartilage was transplanted ectopically, chondrocytes transformed into osteocyte‐like cells accompanying the extracellular calcified matrix. However, we could not determine whether the osteocyte‐like cells were derived from host tissues or from Meckel's cartilage itself. Therefore, we examined whether the Meckel's cartilage chondrocytes, which have a retrogressive ultimate fate, are capable of inducing the observed calcification and further transform into osteocyte‐like cells in culture. Methods Meckelian chondrocytes isolated enzymatically were plated at a low density and grown in α‐MEM containing 10% FBS at 37°C under 5% CO2 in air for up to 4 weeks. Results Chondrocytes were fibroblast‐like cells early in culture, but gradually transformed from polygonal cells into typical chondrocytes showing metachromasia with toluidine blue staining. After an additional week of culture, the chondrocytes transformed from large to small round cells accompanying nodule formations. Small round cells multiple‐layered actively, and showed more intense alkaline phosphatase (ALPase) activity. Immunostaining identified type II collagen in the extracellular matrix at 2 weeks of culture, and type I collagen and osteocalcin were later synthesized by round cells. von Kossa's reaction showed extensive precipitation of calcification throughout the flocculent materials. Ultrastructural analysis showed that the cells surrounded by calcified matrix strongly resembled osteocytes. Conclusions The present study suggested that the Meckel's cartilage chondrocytes can express the osteocyte‐like phenotype in vitro during synthesis of bone‐type marker proteins such as osteocalcin or type I collagen. © 1996 Wiley‐Liss, Inc.

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“…These observations reemphasize the necessity for rigid control of experimental design when comparing parameters for different cell types under varying culture conditions. Ascorbic acid has been shown to stimulate sulfated proteoglycan biosynthesis in chick embryo fibroblasts (46), have no effect in rabbit chondrocytes (44), and to cause decreased sulfate incorporation in human articular cartilage chondrocytes (47).…”

Section: Resultsmentioning

confidence: 99%

Effect of ascorbic acid on arylsulfatase activities and sulfated proteoglycan metabolism in chondrocyte cultures.

Schwartz¹,

Adamy²

1977

J. Clin. Invest.

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A B S T R A C T A correlation between increased arylsulfatase activities and decreased sulfated proteoglycan content in human osteoarthritic articular cartilage suggested a possible interrelationship between these parameters. Since we had previously shown that ascorbate caused a decrease in levels of arylsulfatase A and B activities in normal chondrocyte cultures, the validity of the above relationship was examined by measuring the effect of vitamin C on the biosynthesis and distribution of 35S-labeled proteoglyeans and arylsulfatase A and B activities in cell extracts of chondrocytes derived from normal and osteoarthritic tissue.Arylsulfatase A and B activities were found to be reduced in the presence of ascorbic acid in all normal and osteoarthritic cell lines examined when measured 3, 6, 10, and 13 days after the introduction of the vitamin in the culture medium. Acid phosphatase activity, on the other hand, was found to be elevated in the presence of ascorbate.The inhibitory effect by ascorbic acid on arylsulfatase activities could be reversed by withdrawing the vitamin from the nutrient medium. Addition of EDTA to the cell extracts before assay also reversed the inhibition.Sulfated proteoglycan biosynthesis as reflected in 35S-sulfate uptake per milligram of DNA was significantly increased in the presence of ascorbic acid. The distribution of the newly synthesized molecules between the cell layer and medium fractions was altered. In the presence of ascorbate, more deposition into the cell layer of newly synthesized macromolecules occurred.These data suggest an inverse relationship between arylsulfatase activities and the stability of the newly synthesized sulfated proteoglycans in the extracellular matrix.

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Kinetics of matrix synthesis in cartilage cell cultures*1

Cited by 41 publications

References 21 publications

Preliminary experience with cell culture of human articular chondrocytes

Preliminary experience with cell culture of human articular chondrocytes

Mouse Meckel's cartilage chondrocytes evoke bone-like matrix and further transform into osteocyte-like cells in culture

Effect of ascorbic acid on arylsulfatase activities and sulfated proteoglycan metabolism in chondrocyte cultures.

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