Kinetics of neuropeptide Y, catecholamines, and physiological responses during moderate and heavy intensity exercises

Eugster, Philippe J.; Bourdillon, Nicolas; Vocat, Céline; Wuerzner, Grégoire; Nguyen, Toan D.; Millet, Grégoire P.; Grouzmann, Eric

doi:10.1016/j.npep.2022.102232

Cited by 9 publications

(15 citation statements)

References 41 publications

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“…Finally, the sweat sensor was used to simultaneously detect the three biomarkers in sweat during three trials from healthy volunteers undergoing routine activities including exercise, AA ingestion, and Na + ingestion. It has been reported that exercise activities can trigger NPY secretion in an intensity-dependent manner . To evaluate the capability of sensing NPY in practical use, the NPY analysis in sweat was carried out using the sensor on male volunteers who engaged in a 1 h running.…”

Section: Resultsmentioning

confidence: 99%

A Portable Sweat Sensor Based on Carbon Quantum Dots for Multiplex Detection of Cardiovascular Health Biomarkers

et al. 2022

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The future of personalized diagnostics and treatment of cardiovascular diseases lies in the use of portable sensors. Portable sensors can acquire biomarker information in biological fluids such as sweat, an approach that mitigates the shortcomings of conventional hospital-centered healthcare. Low sensitivity, selectivity, and specificity remain bottlenecks for the widespread use of portable sensors. Herein, we demonstrate a portable sensor that simultaneously detects Na+, ascorbic acid, and human neuropeptide Y in sweat, all useful biomarkers to index cardiovascular health. The portable sensor comprises a six-electrode system containing three working electrodes, two reference electrodes, and one counter electrode. The working electrodes were prepared by depositing sensing components on carbon quantum dot (CQD) electrodes. The sensing mechanisms were based on selective ion recognition, enzyme catalytic reaction, and immune response, which guarantees specificity to corresponding targets. The CQDs offer massive reactive sites and effectively reduce the interfacial impedance during the sensing reaction, thereby enhancing the three biomarkers’ detection sensitivity. As evidence of portable sensor capability, we demonstrate herein its effective simultaneous detection of the three biomarkers in a real sweat from healthy volunteers during routine activities including exercise, extra ascorbic acid ingestion, and extra Na+ ingestion. As such, the sensor shows promise for real-time noninvasive personalized medical diagnostics and metabolic wellness management.

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Section: Resultsmentioning

confidence: 99%

A Portable Sweat Sensor Based on Carbon Quantum Dots for Multiplex Detection of Cardiovascular Health Biomarkers

et al. 2022

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“…However, DPP4 is involved in numerous mechanisms beyond NPY, and the current study cannot rule out that factors we did not measure were involved. The specific assessments of NPY1‐36 and NPY3‐36 during exercise were performed using a unique newly validated technique (Eugster et al, 2022 ; Vocat et al, 2020 ). The present study is the first to report saxagliptin might be used as a potential doping agent, but caution is required because the 32% increase in time‐to‐exhaustion resulted in a smaller time‐trial performance improvement and may not translate into improved performance for elite athletes.…”

Section: Discussionmentioning

confidence: 99%

“…During familiarization, a maximal incremental test to exhaustion was performed on a cycle ergometer to determine peak power output, maximal heart rate (HRmax), and maximal oxygen consumption (V̇O 2max ), as detailed elsewhere (Eugster et al, 2022 ). Briefly, the participants were equipped with a heart rate monitor (Polar H7, Polar electro, Kempele, Finland) to measure R‐R intervals from which the heart rate variability indices were computed.…”

Section: Methodsmentioning

confidence: 99%

“…All procedures are described elsewhere (Eugster et al, 2022 ). The two experimental sessions were performed similarly, except for drug ingestion.…”

Section: Methodsmentioning

confidence: 99%

“…The insertion of a peripheral venous catheter allowed the collection of blood samples for assay of DPP4, NPYs, and CATs (Bergmann et al, 2017 ; Eugster et al, 2022 ; Vocat et al, 2020 ) of 2.6 ml each. The first blood sample of each experimental session was collected in the supine position for determination of DPP4 activity expressed as unit (U) per L of plasma (1 U was 1 μmol of substrate cleaved per min).…”

Section: Methodsmentioning

confidence: 99%

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Saxagliptin: A potential doping agent? A randomized, double‐blinded, placebo‐controlled, and crossover pilot study in young active men

Bourdillon

Eugster

Vocat

et al. 2022

Physiological Reports

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Neuropeptide Ys (NPYs) contribute to sympathetic‐adreno stimulation: NPY1‐36 potentiates the effects of catecholamines (CATs), whereas NPY3‐36 inhibits CAT release. We sought to investigate whether inhibiting dipeptidyl‐peptidase‐4 (DPP4), cleaving NPY1‐36 into NPY3‐36, leads to increased NPY1‐36 potentiating effects and reduced NPY3‐36 inhibitory effects on CATs, thereby improving endurance performance. Seven male participants (age 27 ± 3 years, BMI 23.1 ± 2.4 kg/m 2 ) performed time‐to‐exhaustion cycling exercise at 95% of peak power output with either placebo, or saxagliptin, a DPP4 inhibitor. Oxygen consumption (V̇O 2 ), heart rate variability, NPY1‐36, NPY3‐36, catecholamines, and lactate were measured at several time points before, during, and after exercise. With saxagliptin, DPP4 activity (12.7 ± 1.6 vs. 0.2 ± 0.3 U/L, p = 0.001; d = 10.7) was decreased at rest, while NPY3‐36 (1.94 ± 0.88 vs. 0.73 ± 0.22 pm ; p < 0.001; d = 2.04) decreased and NPY1‐36 increased during exercise (2.64 ± 2.22 vs. 4.59 ± 2.98 pm ; p < 0.01; d = 0.19). CATs were unchanged. Time‐to‐exhaustion was 32% higher with saxagliptin. The difference in time‐to‐exhaustion between placebo and saxagliptin was correlated with NPY1‐36 differences (R = 0.78, p < 0.05). Peak V̇O 2 and other cardio‐respiratory values were not different, whereas peak NPY concentrations were higher with saxagliptin. DPP4 blockade improved performance, increased NPY1‐36, and decreased NPY3‐36 concentrations which may have potentiating effects on the influences of CATs. However, DPP4 is involved in many different actions, thus NPYs are one group of factors that may underly its performance‐enhancing effects; further studies are required to determine the exact mechanisms.

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Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

Ong

Green

Rowland

et al. 2023

Analytical Science Advances

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Research in sport and exercise science (SES) is reliant on robust analyses of biomarker measurements to assist with the interpretation of physiological outcomes. Mass spectrometry (MS) is an analytical approach capable of highly sensitive, specific, precise, and accurate analyses of a range of biomolecules, many of which are of interest in SES including, but not limited to, endogenous metabolites, exogenously administered compounds (e.g. supplements), mineral ions, and circulating/tissue proteins. This annual review provides a summary of the applications of MS across studies investigating aspects related to sport or exercise in manuscripts published, or currently in press, in 2022. In total, 93 publications are included and categorized according to their methodologies including targeted analyses, metabolomics, lipidomics, proteomics, and isotope ratio/elemental MS. The advantageous analytical opportunities afforded by MS technologies are discussed across a selection of relevant articles. In addition, considerations for the future of MS in SES, including the need to improve the reporting of assay characteristics and validation data, are discussed, alongside the recommendation for selected current methods to be superseded by MS‐based approaches where appropriate. The review identifies that a targeted, mostly quantitative, approach is the most commonly applied MS approach within SES, although there has also been a keen interest in the use of ‘omics’ to perform hypothesis‐generating research studies. Nonetheless, MS is not commonplace in SES at this time, but its use to expand, and possibly improve, the analytical options should be continually considered to exploit the benefits of analytical chemistry in exercise/sports‐based research. Overall, it is exciting to see the gradually increasing adoption of MS in SES and it is expected that the number, and quality, of MS‐based assays in SES will increase over time, with the potential for 2023 to further establish this technique within the field.

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Kinetics of neuropeptide Y, catecholamines, and physiological responses during moderate and heavy intensity exercises

Cited by 9 publications

References 41 publications

A Portable Sweat Sensor Based on Carbon Quantum Dots for Multiplex Detection of Cardiovascular Health Biomarkers

A Portable Sweat Sensor Based on Carbon Quantum Dots for Multiplex Detection of Cardiovascular Health Biomarkers

Saxagliptin: A potential doping agent? A randomized, double‐blinded, placebo‐controlled, and crossover pilot study in young active men

Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

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