Kinetics of optical properties of human colorectal tissues during optical clearing: a comparative study between normal and pathological tissues

Carneiro, Isa; Carvalho, Sónia; Silva, Vânia; Henrique, Rui; Oliveira, Luís; Tuchin, Valery V.

doi:10.1117/1.jbo.23.12.121620

Cited by 21 publications

(19 citation statements)

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“…where a is a scaling factor that represents µ s or µ s at 500 nm, f Ray is the Rayleigh scattering fraction, and b Mie characterizes the mean size of Mie scatterers. Such equation has been successfully applied to fit the wavelength dependence for data of many biological soft tissues [20][21][22]. Equation (3) describes a smooth wavelength dependence for both scattering coefficients in the spectral range from UV to NIR.…”

Section: Introductionmentioning

confidence: 99%

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

et al. 2020

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The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method, and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

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Section: Introductionmentioning

confidence: 99%

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

et al. 2020

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“…where a is a scaling factor that represents s or s at 500 nm, fRay is the Rayleigh scattering fraction and bMie characterizes the mean size of Mie scatterers. Such equation has been successfully applied to fit the wavelength dependence for data of many biological soft tissues [20][21][22]. Equation (3) describes a smooth wavelength dependence for both scattering coefficients in the spectral range from UV to NIR.…”

Section: Introductionmentioning

confidence: 99%

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Carvalho¹,

Carneiro²,

Henrique³

et al. 2020

Preprint

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The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

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“…(6) and the depth of penetration of collimated radiation, calculated using Eqs. (7) and (8) Spectral range 500-600 nm 600-700 nm 700-900 nm OCE (500.0±2.5)×10 -4 (810.0±3.6)×10 -4 (1310±4)×10 -4 δ c t = 0 ( ) , µm 83. The results of measurements of the efficiency of optical clearing and the change in the depth of collimated radiation penetration in the three spectral ranges are presented in Table 4.…”

Section: Resultsmentioning

confidence: 99%

“…Among substances used as OCAs, a special place is occupied by aqueous solutions of glucose of various concentrations due to their biocompatibility and low cost [8][9][10][11][12][13][14][15][16][17][18][19][20]. Most often, glucose solutions are used for the optical clearing of connective tissues, such as skin dermis, sclera, dura mater, etc.…”

Section: J Of Biomedical Photonics and Eng 5(3)mentioning

confidence: 99%

“…As shown in numerous studies (see, for example, Refs. [8][9][10][11][12][13][14][15]), the scattering properties of biological tissues can be effectively controlled by action of hyperosmotic immersion optical clearing agents (OCAs), which cause diffusion of water from interstitial space and partially replace the interstitial fluid. This method is known in literature as a "tissue optical clearing" technique.…”

Section: Introductionmentioning

confidence: 99%

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Optical Clearing of the Gastric Mucosa Using 40%-glucose Solution

Genin

Genina

Капралов

et al. 2019

J-BPE

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The kinetics of collimated transmittance of the gastric mucosa under the action of an aqueous 40%-glucose solution was experimentally investigated. Based on the analysis of the transmittance kinetics, the value of the effective diffusion coefficient of glucose in the gastric mucosa was estimated and amounted to (1.59±0.96)×10-6 cm 2 /s. The permeability coefficient of the mucosa for glucose, calculated using the first Fick diffusion law, was estimated as (2.81±0.90)×10-5 cm/s. It was shown that the introduction of the glucose solution into the mucosa reduced the light scattering coefficient by approximately 5-10%. The increase in the depth of light penetration was from 5% to 15%, depending on the selected spectral range. The results can be used to develop new methods of diagnosis and treatment of stomach diseases.

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Kinetics of optical properties of human colorectal tissues during optical clearing: a comparative study between normal and pathological tissues

Cited by 21 publications

References 44 publications

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Optical Clearing of the Gastric Mucosa Using 40%-glucose Solution

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