Kinetics of pesticide degradation by human fresh frozen plasma (FFP) in vitro

Wellen, Jens von der; Bierwisch, Anne; Worek, Franz; Thiermann, Horst; Wille, Timo

doi:10.1016/j.toxlet.2015.07.014

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…Purified rabbit serum was injected into the tail veins of rats, increasing the peak hydrolytic activity of rat serum toward paraoxon by 9-fold and increasing that toward chlorpyrifos-oxon by 50-fold (21). The degradation of human fresh-frozen plasma was rapid, with half-lives of 19.5 s for chlorpyrifos-oxon and 17.9 min for DDVP (22). Engineered HuPON1 in the E. coli expression system showed kinetic parameters ( Km and V max) of 0.121-0.317 mM and 34.7-245 U mg −1 protein for chlorpyrifos-oxon and 0.957-3.22 mM and 3020-680 U mg −1 for phenyl acetate (23, 24).…”

Section: Resultsmentioning

confidence: 99%

“…PON1s derived from mammals such humans, rabbits, and rats demonstrated the metabolic function of removing organophosphate pesticide residues from blood (33). Degradation of human fresh-frozen plasma containing high levels of HuPON1 was rapid, with the shortest half-life of 17.9 min for DDVP (22). Injection of PON1 into rats with acute organophosphate poisoning can decrease the amount of DDVP that enters the blood, lower the peak concentration, and relieve clinical signs(34).…”

Section: Discussionmentioning

confidence: 99%

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The biochemical properties of a novel paraoxonase-like enzyme in Trichoderma atroviride strain T23 involved in the degradation of 2,2-dichlorovinyl dimethyl phosphate

Sun

et al. 2018

Preprint

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Dichlorvos, is a broad-spectrum organophosphorus pesticide that is widely applied in the agricultural industry and considered a pollutant to fish and bees. T. atroviride strain T23, an efficient DDVP-degrading strain, could convert DDVP to dichloroacetic acid, 2,2-dichloroethanol and phosphoric acid through mineralization. RT-qPCR analysis showed TaPon1-like encoding an organophosphorus hydrolase, is continuously highly expressed in the process of degrading DDVP. TaPon1-like contained an open reading frame of 1317 bp, and the deduced amino acid sequence shared 21% homology with HuPON1, which also exhibits excellent hydrolysis of organophosphate-oxons compounds. By analysis of gene knockout, we found the ΔTaPon1-like knockout strain KO1 lost 35.6% of its DDVP-degradation capacity at 24 h, but this loss of degradation activity was recovered when the gene was complemented. Furthermore, the purified recombinant protein reTAPON1-LIKE, could transform DDVP only to dimethyl phosphate and showed significant paraoxonase activity (1028 U L−1). The reTAPON1-LIKE enzyme showed a broad degradation spectrum, degrading not only DDVP but also organophosphate-oxons and lactone. The kinetic parameters (Km and kcat) of the purified reTAPON1-LIKE were determined to be 0.23 mM and 204.3 s−1 for DDVP, respectively. The highest activity was obtained at 35 °C, and the optimal pH was 8.5. The activity of reTAPON1-LIKE was enhanced most significantly when 1.0 mM Ca2+ was added but declined when 1.0 mM Cu2+ was added. These results showed TAPON1-LIKE play an important role for DDVP degradation in the first step by T23 and provided clue to comprehensively understanding the degradation mechanism of organophosphate-oxons pesticides by filamentous fungi.ImportanceThe large amounts of residues of organophosphate pesticides in agroecological system has become a great threat to the safety of environment and humans. Bioremediation in association with microbial is innovative technology having a potential to alleviate such pollution problems. The genus Trichoderma is genetically diverse with capabilities to degrade chemical pesticides among different strains with agricultural significance. As a typical organophosphorus pesticide, it is one of the most employed compounds of the family. Though it was classified as a highly toxic pesticide by WHO due to its hazardous properties, it plays an important role in the control of plant pests, food storage and homes, as well as to treat infections in livestock. Therefore, we use DDVP as a model of organophosphate pesticide to study the mechanism of Trichoderma degrading organophosphate pesticides, for the aim of globally understanding molecular mechanism of enzymatic degradation of organophosphate pesticides by beneficial fungi.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The biochemical properties of a novel paraoxonase-like enzyme in Trichoderma atroviride strain T23 involved in the degradation of 2,2-dichlorovinyl dimethyl phosphate

Sun

et al. 2018

Preprint

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show abstract

“…Purified rabbit serum was injected into the tail veins of rats, increasing the peak hydrolytic activity of rat serum toward paraoxon by 9-fold and increasing that toward chlorpyrifos-oxon by 50-fold (21). The degradation of human fresh-frozen plasma was rapid, with half-lives of 19.5 s for chlorpyrifos-oxon and 17.9 min for DDVP (22). Engineered HuPON1 in the E. coli expression system showed kinetic parameters (K m and V max ) of 0.121-0.317 mM and 34.7-245 U mg -1 protein for chlorpyrifos-oxon and 0.957-3.22 mM and 3020-680 U mg -1 for phenyl acetate (23,24).…”

Section: Heterologous Expression and Purification Of Tapon1-likementioning

confidence: 99%

The pathway of 2,2-dichlorovinyl dimethyl phosphate (DDVP) degradation by Trichoderma atroviride strain T23 and characterization of a paraoxonase-like enzyme

Sun

et al. 2019

Appl Microbiol Biotechnol

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“…In addition, FFP provides other important molecules, such as esterases and proteases, which may counteract the OP effects. How successfully will depend on the type of OP pesticides (18). Further research is therefore needed to better understand the effects of BuChE on the central nervous system (17).…”

Section: Case Reportmentioning

confidence: 99%

New insights into the organophosphate-induced intermediate syndrome

Haliga

Morăraşu²,

Ursaru³

et al. 2018

Archives of Industrial Hygiene and Toxicology

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Acute organophosphate (OP) poisoning can be deadlier than any other type of chemical poisoning. So far, only a few cases have been described that include extensive neurological complications. We present an outstanding case of severe oral OP poisoning with intermediate syndrome developed on the fourth day after hospital admission. The clinical picture involved weakness of the proximal upper and lower limb muscles and several muscles supplied by motor cranial nerves, but, what is peculiar, the distal upper and lower limb muscles were also affected (forearms, hands, legs, and feet). To our knowledge, this is a unique presentation, as lower limb muscle weakness was reported only in the context of delayed polyneuropathy. Another remarkable feature was the involvement of six of the twelve cranial nerves, which makes this case of intermediate syndrome the first with such a spread of muscle weakness and provides new insights into the polymorphic clinical manifestations of acute OP poisoning.

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Kinetics of pesticide degradation by human fresh frozen plasma (FFP) in vitro

Cited by 9 publications

References 26 publications

The biochemical properties of a novel paraoxonase-like enzyme in Trichoderma atroviride strain T23 involved in the degradation of 2,2-dichlorovinyl dimethyl phosphate

The biochemical properties of a novel paraoxonase-like enzyme in Trichoderma atroviride strain T23 involved in the degradation of 2,2-dichlorovinyl dimethyl phosphate

The pathway of 2,2-dichlorovinyl dimethyl phosphate (DDVP) degradation by Trichoderma atroviride strain T23 and characterization of a paraoxonase-like enzyme

New insights into the organophosphate-induced intermediate syndrome

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