Kinetics of RANKL, RANK and OPG expressions in experimentally induced rat periapical lesions

Kawashima, Nobuyuki; Suzuki, Noriyuki; Yang, Guangren; Ohi, chie; Okuhara, Shigeru; Nakano-Kawanishi, Hiromi; Suda, Hiroshi

doi:10.1016/j.tripleo.2006.11.036

Cited by 85 publications

(95 citation statements)

References 31 publications

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“…Previous studies performed in rats (20) and humans (21) suggested that MMP2 and MMP9 could be acting in the ECM degradation, favoring the lesion progression, and together with other cytokines, contributing to bone destruction in apical periodontitis. MMP2 modulates many physiological conditions including wound repair and tissue remodeling (22).…”

Section: Discussionmentioning

confidence: 98%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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The aim of this study was to evaluate the expression of MMP2 and MMP9 during apical periodontitis (AP) progression in TLR2 (TLR2 KO) and in MyD88 (MyD88 KO) knockout mice compared to wild type (WT) mice. AP was induced in mandibular first molars of TLR2 KO (n= 18), MyD88 KO (n= 18), and WT mice (n= 18). After 7, 21, and 42 days, the animals were euthanized and the jaws were dissected and subjected to histotechnical processing. Subsequent sections were stained by immunohistochemistry and evaluated for detection of MMP2 and MMP9. Statistical analysis of the semi-quantitative analysis of immunohistochemistry was performed using chi-square test (α = 0.05). In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression. MMP2 and MMP9 are Associated w i t h A p i c a l P e r i o d o n t i t i s P r o g r e s s i o n a n d M i g h t b e Modulated by TLR2 and MyD88

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Section: Discussionmentioning

confidence: 98%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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“…This may reflect the nature of the respective diseases, in which these cells are involved. To this extent, RANKL in periapical lesions has been particularly associated with fibroblastic cells, rather than T-cells [25], as opposed to periodontitis, where cells of the immune system are considered a major source of RANKL [9,26,27]. When the relative RANKL/OPG ratio was calculated, this was significantly increased in DP cells, compared to PDL cells, indicating a higher capacity for inducing bone resorption by the former cells.…”

Section: P O R P H Y R O Mo N a S G I N G I V A L I S Have Been Stumentioning

confidence: 99%

Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells

Belibasakis

Meier

Guggenheim

et al. 2011

Microbial Pathogenesis

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“…Los autores sugieren que la MMP-2 podría representar un marcador útil para el monitoreo de PAa en el FGC (10) . Consecuentemente, la MMP-2 podría estar actuando en la degradación de la MEC, favoreciendo la expansión de la lesión y, junto con algunas citoquinas, como lo son TNF-, IL-1 , IL-1 , y el sistema RANKL/RANK/OPG (23) , podría contribuir también a la destrucción ósea (22) . En relación a la MMP-9, su detección fue similar a estudios anteriores, donde también se describió su expresión en PMN, linfocitos, plasmocitos, células endoteliales y epiteliales (16,22,24) .…”

Section: Discussionunclassified

Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomática

Burgos

Piwonka

Alfaro

et al. 2011

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomáticaImmunodetection of matrix metalloproteinases (MMPs)-2, -9, -13 and -14 in periapical lesions associated with asymptomatic apical periodontitis RESUMENLa periodontitis apical asintomática (PAa) es una patología infecciosa caracterizada por destrucción ósea perirradicular asociada a un proceso inflamatorio crónico y producción de mediadores inflamatorios, entre los cuales se encuentran las metaloproteinasas de matriz extracelular (MMPs).Entre éstas, las MMPs-13, -14, -2 y -9, son producidas por el tejido óseo y degradan sinérgicamente el colágeno tipo I, principal componente de los tejidos periodontales, y gelatina, producto de la degradación y desnaturación del colágeno. El objetivo de este estudio fue determinar el patrón de expresión de las MMPs-2, -9, -13 y -14 en granulomas periapicales (GPAs), quistes radiculares inflamatorios (QRIs) y ligamento periodontal sano (LS). Materiales y Métodos:Se seleccionaron 12 pacientes con diagnóstico clínico de PAa e indicación de exodoncia a partir de los cuales se obtuvieron biopsias de lesiones periapicales (LPAs). Como controles, se seleccionaron 7 individuos con indicación de exodoncia de premolares por ortodoncia, obteniéndose biopsias de LS. Se efectuó el diagnóstico anátomo-patológico de los especímenes y se caracterizó la expresión de las MMPs en estudio mediante inmunohistoquímica. Resultados: Las MMPs en estudio sólo se detectaron en GPAs y QRIs, y se inmunolocalizaron principalmente en el infiltrado inflamatorio de éstos. Adicionalmente, la MMP-2 se identificó en fibroblastos del tejido conectivo. Conclusiones: MMPs-2, -9, -13 y -14 se expresan predominantemente en el infiltrado inflamatorio de las LPAs y no en LS, y por tanto se sugiere la participación de estos mediadores en la patogénesis de la PAa. Rev. Clin. Periodoncia Implantol. Rehabil. Oral Vol. 4(1); 17-21, 2011.Palabras clave: Periodontitis apical asintomática, lesiones periapicales, metaloproteinasas de matriz extracelular. ABSTRACTAsymptomatic apical periodontitis (aAP) is an infectious disease characterized by perirradicular bone destruction associated with chronic inflammation and release of inflammatory mediators, such as matrix metalloproteinases (MMPs). MMPs-13, -14 and -2, -9 are bone-expressed enzymes that can synergistically degrade collagen I, the main component of periodontal extracellular matrix, and gelatin, the product of degradation and denaturation of collagen. The aim of this study was to characterize the expression pattern of MMPs-2, -9, -13, and -14 in periapical granulomas (PGs), radicular cysts (RCs) and healthy periodontal ligament (PDL). Materials and Methods: Individuals with clinical diagnosis of aAP and indication of extraction were selected (N=12), and biopsies of periapical lesions (PLs) were obtained. For controls, 7 subjects with indication of premolar extraction for orthodontic reasons were selected, and PDL biopsies wer...

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Kinetics of RANKL, RANK and OPG expressions in experimentally induced rat periapical lesions

Cited by 85 publications

References 31 publications

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells

Inmunodetección de metaloproteinasas de matriz extracelular (MMPs)-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomática

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