Kinetics of singlet oxygen photosensitization in human skin fibroblasts

Jiménez-Banzo, Ana; Sagristá, Maria Lluïsa; Mora, Marı́a de la Luz; Nonell, Santi

doi:10.1016/j.freeradbiomed.2008.02.011

Cited by 82 publications

(100 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both these photochemical reactions can occur simultaneously to produce ROS and excited state singlet oxygen, which are the primary cytotoxic agents responsible for the eradication of cancerous tissue (tumor) making PDT highly dependent on the amount of ROS and excited state singlet oxygen produced by these photochemical reactions. [3][4] Phthalocyanines (Pcs) are industrial dyes 5 which have proven to be promising as photosensitizing agents for PDT, due to its versatility, chemical and thermal stability. [6][7] The PDT properties of the Pc dyes can be influenced by the presence and nature of the incorporated metal ion.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of gallium phthalocyanine as a photosensitizing agent in photodynamic therapy for the treatment of cancer

Maduray

Odhav

2012

SPIE Proceedings

View full text Add to dashboard Cite

Photodynamic therapy is a revolutionary treatment aimed at treating cancers without surgery or chemotherapy. It is based on the discovery that certain chemicals known as photosensitizing agents (e.g. porphyrins, phthalocyanines, etc.) can kill cancerous cells when exposed to low level laser light at a specific wavelength. The present study investigates the cellular uptake and photodynamic effect of gallium (III) phthalocyanine chloride (GaPcCl) on Caco-2 cancer cells. Caco-2 cells were treated with different concentrations of GaPcCl for 2 h before treatment with a diode laser (λ = 661 nm, laser power = 90 mW) delivering a light dose of 2.5 J/cm 2 , 4.5 J/cm 2 or 8.5 J/cm 2 . After 24 h, the cell viability of post-irradiated cells was measured using the MTT assay. Cellular uptake studies were performed by photosensitizing cells with GaPcCl for 30 min, 2 h, 10 h, 12 h, 18 h and 24 h before lysing the treated cells into solution to measure the GaPcCl fluorescence emission at an excitation wavelength of 600 nm. Results showed an increase in fluorescence intensity of emission peaks at longer incubation times, indicating a greater cellular uptake of GaPcCl by Caco-2 cells at 24 h in comparison to 30 min. GaPcCl at a concentration of 100 µg/ml activated with a laser light dose of 8.5 J/cm 2 reduced the cell viability of Caco-2 cells to 27%. This concludes that GaPcCl activated with low level laser light can be used as a photosensitizing agent for the in vitro PDT treatment of colon cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficacy of gallium phthalocyanine as a photosensitizing agent in photodynamic therapy for the treatment of cancer

Maduray

Odhav

2012

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

“…Moreover, simple symmetric porphyrins are low-cost materials and depending on the application, demonstrate high efficiency, such as for optical devices [1][2][3][4][5]. Specifically, porphyrins/micelles interaction is of interest because micelles can been considered as a model of biological membrane [18][19][20], and, also because they as a matrix for active optical molecular systems [13,21].…”

Section: Introductionmentioning

confidence: 99%

“…TMPyP has also demonstrated anti-HIV [21,23] and antibacterial activity [18], and it was used as active compound for singlet oxygen imaging of single cells [19] and for singlet oxygen photosensitization in skin fibroblasts [20]. Although TMPyP has already been demonstrated to present nonlinear optical response [24], there is a lack of information how this molecule interacts with the distinct environments, such as organic solvents and micelles, and consequently, how its excited state dynamics is altered.…”

Section: Introductionmentioning

confidence: 99%

Effects of environment on the photophysical characteristics of mesotetrakis methylpyridiniumyl porphyrin (TMPyP)

Gonçalves

Franzen

Corrêa

et al. 2011

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

“…The therapy also has indirect effects arising from the destruction of tumor vasculature. 55,56 The most widely used PCs are compounds of the porphyrin group, such as the PC derived from hematoporphyrin used for the treatment of localized tumors accessible to light and neoplasms in the lungs, bladder, esophagus, and, in particular, the skin. 54 Other new PCs approved for clinical use are the hematoporphyrin dihydrochloride and PC precursors such as 5-aminolevulinic acid (ALA) and its methylated derivative (MAL).…”

Section: New Therapeutic Approaches In Skin Diseases: Photodynamic Thmentioning

confidence: 99%

New Experimental Models of Skin Homeostasis and Diseases

Larcher¹,

Espada

Díaz-Ley

et al. 2015

Actas Dermo-Sifiliográficas (English Edition)

View full text Add to dashboard Cite

Homeostasis, whose regulation at the molecular level is still poorly understood, is intimately related to the functions of epidermal stem cells. Five research groups have been brought together to work on new in vitro and in vivo skin models through the SkinModel-CM program, under the auspices of the Spanish Autonomous Community of Madrid. This project aims to analyze the functions of DNA methyltransferase 1, endoglin, and podoplanin in epidermal stem cell activity, homeostasis, and skin cancer. These new models include 3-dimensional organotypic cultures, immunodeficient skin-humanized mice, and genetically modified mice. Another aim of the program is to use skin-humanized mice to model dermatoses such as Gorlin syndrome and xeroderma pigmentosum in order to optimize new protocols for photodynamic therapy.

show abstract

Kinetics of singlet oxygen photosensitization in human skin fibroblasts

Cited by 82 publications

References 62 publications

Efficacy of gallium phthalocyanine as a photosensitizing agent in photodynamic therapy for the treatment of cancer

Efficacy of gallium phthalocyanine as a photosensitizing agent in photodynamic therapy for the treatment of cancer

Effects of environment on the photophysical characteristics of mesotetrakis methylpyridiniumyl porphyrin (TMPyP)

New Experimental Models of Skin Homeostasis and Diseases

Contact Info

Product

Resources

About