Kinetics of the HCG-induced Steroidogenic Response of the Human Testis. III. Studies in Children of the Plasma Levels of Testosterone and HCG: Rationale for Testicular Stimulation Test

Forest, Maguelone G.; Dávid, M; Lecoq, Annick; Jeune, M; Bertrans, Jean

doi:10.1203/00006450-198006000-00009

Cited by 52 publications

(32 citation statements)

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“…In our study, testosterone levels increased to values well above those of infant boys. Prepubertal boys have a monophasic testosterone response to hGH with peak levels 4-5 days after hCG administration (26,27). Four injections of hCG (100 IU/kg at 5-day intervals) produced testosterone levels within the normal adult male range, and similar success rates compared with seven injections of 1500 IU every other day, with no correlation to testosterone levels (28).…”

Section: Discussionmentioning

confidence: 99%

Serum inhibin B, FSH, LH and testosterone levels before and after human chorionic gonadotropin stimulation in prepubertal boys with cryptorchidism

Christiansen

Andersson²,

Ne³

et al. 2002

Eur J Endocrinol

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Background: Several studies have indicated that cryptorchidism is associated with degenerative changes in both Sertoli cells and germ cells. The gonadal peptide hormone inhibin B reflects Sertoli cell function. Low inhibin B levels are found in a large portion of formerly cryptorchid men who show compromised seminiferous tubule function. It is not known if inhibin B can be used to demonstrate early damage of seminiferous tubules in prepubertal boys with cryptorchidism. Methods: We investigated the relationship between serum levels of inhibin B, testosterone, FSH and LH in 62 prepubertal boys with uni-and bilateral cryptorchidism. Furthermore, we investigated the changes in serum levels of inhibin B and the corresponding changes in serum levels of FSH, LH and testosterone during a short course (3 weeks) of human chorionic gonadotropin (hCG) injections in 18 of these cryptorchid boys. Results: In the 62 prepubertal boys with uni-or bilateral cryptorchidism there were no significant differences in baseline levels (median and range) of inhibin B (88 (20-195) pg/ml vs 78 (35-182) pg/ml; not significant), LH (0.08 (,0.05 -0.99) IU/l vs 0.06 (,0.05 -1.61) IU/l; not significant) and FSH (0.60 (0.08-3.73) IU/l vs 0.85 (0.25 -2.55); not significant) compared with 156 healthy prepubertal boys, and there were no differences in hormonal levels between boys with unior bilateral cryptorchidism. There was no correlation between baseline levels of inhibin B and FSH. In boys younger than 9 years, we found no correlation between baseline levels of inhibin B and LH whereas, in boys older than 9 years, baseline levels of inhibin B were positively correlated to baseline LH (Spearman rank correlation coefficient ððR s Þ ¼ 0:58; P ¼ 0:03Þ: Treatment with hCG (1500 IU intramuscularly twice weekly for 3 weeks) resulted in descensus of testes in 9 out of 18 patients. In all boys but one, irrespective of age, hCG induced a marked increase in testosterone into the adult range (from undetectable to 21.8 (7.0 -35.4) nmol/l; P , 0:001) and completely suppressed FSH and LH levels. Serum levels of inhibin B increased significantly from 116 (50 -195) pg/ml to 147 (94 -248) pg/ml ðP , 0:05Þ; but not uniformly. The increase in serum levels of inhibin B was inversely correlated to baseline inhibin B ðRs ¼ 20:52; P ¼ 0:03Þ and baseline FSH ðR s ¼ 20:59; P , 0:01Þ: Conclusions: We therefore suggest that, in the prepubertal testes, inhibin B is secreted from the prepubertal Sertoli cells following hCG, whereas early pubertal testes with more differentiated Sertoli cells are not able to secrete inhibin B in response to hCG stimulation, perhaps due to lack of germ cell-derived bB-subunits. We found (a) normal inhibin B levels in prepubertal boys with uni-or bilateral cryptorchidism, (b) that hCG stimulated testosterone markedly and suppressed FSH and LH levels and (c) that hCG treatment stimulated inhibin B levels in the youngest cryptorchid boys. In the oldest prepubertal boys no hCG-induced changes in inhibin B were shown.

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Section: Discussionmentioning

confidence: 99%

Serum inhibin B, FSH, LH and testosterone levels before and after human chorionic gonadotropin stimulation in prepubertal boys with cryptorchidism

Christiansen

Andersson²,

Ne³

et al. 2002

Eur J Endocrinol

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“…Since a second hCG injection at 24-hour intervals has little [49] or some additional stimulatory effect [50], it would theoretically be unnecessary to repeat the hCG injection before the maximal response has occurred. On the other hand, it has been shown that testosterone levels are comparable after the administration of 4 hCG injections at 5-day [31,49], Therefore, in order to test the above hypotheses, we have compared the effect of these two modes of hCG administration on testicular descent and hormonal response in a population of pre pubertal boys with undescended testes, using the same criteria for selection of patients and estimation of the results.…”

Section: Introductionmentioning

confidence: 99%

Undescended Testis: Comparison of Two Protocols of Treatment with Human Chorionic Gonadotropin

et al. 1988

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A randomized study of two protocols of human chorionic gonadotropin (hCG) treatment was performed in 183 prepubertal boys between 7 months and 12 years of age: protocol I, in which the boys were given 7 injections of 1,500 IU every other day, and protocol II consisting of 4 injections of 100 IU/kg at 4- to 5-day intervals. In both protocols, by the end of the test, testosterone had risen significantly to values within the normal adult male range. However, the amplitude of the rise was slightly but significantly lower using protocol II (4.08 ± 2.07 ng/ml) than protocol I (5.16 ± 2.73 ng/ml). It would thus appear that repetition of the hCG injection at intervals of less than 4 days is unnecessary, and that a total stimulation period of 2–3 weeks is sufficient. Although not correlated with testosterone levels, the success rates for treatment were similar in both protocols and comparable to rates reported in the literature.

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“…At present little is known about the kinetics of the steroidogenic response to repeated doses of hCG in boys (9). It is of particular interest, because current protocols for diagnostic hCG stimulation test include massive and repeated doses of hCG (1 1, 12, 19-21).…”

mentioning

confidence: 99%

Kinetics of the Steroidogenic Response to Single versus Repeated Doses of Human Chorionic Gonadotropin in Boys in Prepuberty and Early Puberty

1985

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Kinetics of the HCG-induced Steroidogenic Response of the Human Testis. III. Studies in Children of the Plasma Levels of Testosterone and HCG: Rationale for Testicular Stimulation Test

Cited by 52 publications

References 1 publication

Serum inhibin B, FSH, LH and testosterone levels before and after human chorionic gonadotropin stimulation in prepubertal boys with cryptorchidism

Serum inhibin B, FSH, LH and testosterone levels before and after human chorionic gonadotropin stimulation in prepubertal boys with cryptorchidism

Undescended Testis: Comparison of Two Protocols of Treatment with Human Chorionic Gonadotropin

Kinetics of the Steroidogenic Response to Single versus Repeated Doses of Human Chorionic Gonadotropin in Boys in Prepuberty and Early Puberty

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