“…In order to assess antioxidant properties of HPEs and separate fractions, we used the method based on the ability to inhibit the ABTS + radical. Kinetics of ABTS + radical cation reduction measured as the percentage inhibition of absorbance at 734 nm includes 2 phases due to the presence of rapid-and slow-reducing antioxidants [9,16]. Activity of antioxidants responsible for rapid reduction (such as ascorbic acid, uric acid, α-tocopherol, amino acids containing SH groups, reduced glutathione, phenol compounds and ubiquinones) was evaluated by decolorization during the first 10 s. Activity of antioxidants responsible for slow reduction was estimated by decay during the next 390 s (Fig.…”