King crab gills as a new source of chitin/chitosan and protein hydrolysates

Novikov, V. Yu.; Rysakova, K.S.; Shumskaya, Nadezhda; Mukhortova, A.M.; Kesarev, K.A.

doi:10.1016/j.ijbiomac.2023.123346

Cited by 16 publications

(2 citation statements)

References 47 publications

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“…We compared the kinetic curves (degree of deacetylation versus time) and physicochemical properties of chitin isolated from the shells of these marine crustaceans. Data published in the scientific literature [ 3 , 7 ] and our own experimental data [ 44 , 47 , 62 , 63 ] show that α-chitin obtained from various raw materials (crab shells, shrimp, Antarctic krill, as well as the internal organs of crustaceans, for example, the gills of the king crab [ 64 ]), is almost identical both in its chemical composition and in physicochemical properties. A similar conclusion was made in [ 65 ].…”

Section: Kinetics Of the Chitin Deacetylation Reactionmentioning

confidence: 99%

Mechanism of Heterogeneous Alkaline Deacetylation of Chitin: A Review

et al. 2023

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This review provides an analysis of experimental results on the study of alkaline heterogeneous deacetylation of chitin obtained by the authors and also published in the literature. A detailed analysis of the reaction kinetics was carried out considering the influence of numerous factors: reaction reversibility, crystallinity and porosity of chitin, changes in chitin morphology during washing, alkali concentration, diffusion of hydroxide ions, and hydration of reacting particles. A mechanism for the chitin deacetylation reaction is proposed, taking into account its kinetic features in which the decisive role is assigned to the effects of hydration. It has been shown that the rate of chitin deacetylation increases with a decrease in the degree of hydration of hydroxide ions in a concentrated alkali solution. When the alkali concentration is less than the limit of complete hydration, the reaction practically does not occur. Hypotheses have been put forward to explain the decrease in the rate of the reaction in the second flat portion of the kinetic curve. The first hypothesis is the formation of “free” water, leading to the hydration of chitin molecules and a decrease in the reaction rate. The second hypothesis postulates the formation of a stable amide anion of chitosan, which prevents the nucleophilic attack of the chitin macromolecule by hydroxide ions.

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Section: Kinetics Of the Chitin Deacetylation Reactionmentioning

confidence: 99%

Mechanism of Heterogeneous Alkaline Deacetylation of Chitin: A Review

et al. 2023

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“…Crab shells are mostly composed of 20-30% chitin, 30-40% protein, 30-50% ash (mainly calcium carbonate) and a small amount of liquid and pigment, which varies with species, season of catching, body parts, sex differences, etc (Hajji et al 2014;Hamed et al 2016;Wang et al 2022;Burke et al 2023;Xing et al 2023). Crab gills were found to be with high content of protein (66.52±0.20%) and low in ash (10.47±0.05%) compared to carapace waste (25.50±0.10% of protein and 51.00±0.10% of ash) (Novikov et al 2023). It is said that the carapace of the freshwater crab Aegla cholchol contains most protein than the exoskeleton, pincers and legs, while the exoskeleton has the maximum ash content.…”

Section: Chemical Composition Of Crab Shellsmentioning

confidence: 99%

Chitin extraction from crab shells by two-step fermentation with Lacticanttacllus pantarum and Pseudomonas aeruginosa

Zhou,

Yang,

Xing

et al. 2024

Preprint

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There are few reports on the extraction of chitin from crab shells using microbiological methods. In this work, the strain Lacticanttacllus pantarum (LA01) for demineralization (DM) and the strain Pseudomonas aeruginosa (PS01) for deproteinization (DP) were screened from soil, seawater, mud of aquaculture farm, seamount, marine cold spring, probiotic powders, etc. After optimizing the fermentation conditions, LA01 showed the highest activity under the conditions of 2% glucose, 1g crab shells of 18 mesh in 100mL system for 48h, while PS01 performed best with 0.5g crab shells of 60 mesh in 100mL system for 48h. chitin was extracted from crab shells using two-step fermentation method with two scheme: (i) LA01 followed by PS01; (ii) PS01 followed by LA01, and the products were analyzed by FTIR, XRD and SEM. Results showed that 97.55 ± 0.15% of DM and 73.49 ± 0.25% of DP were eliminated by inoculating PS01 followed by LA01. The strong connection between protein and chitin in the crab shells was speculated as the reason of protein residues. Similar structure was observed in the fermented product by the results of FTIR and XRD compared with commercial chitin, indicating the structure of α-chitin. The presence of porous and fibrous structures was observed in crab chitin by two-step fermentation using SEM. The fermentation broth acquired from LA01 contained with various organic acids, lactic acid mostly, with contents of 4238.65mg/L, along with 356.91mg/L acetic acid, which has potential application in inhibiting other bacteria pollution during fermentation.

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Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa

Zhou,

Yang,

et al. 2024

Biomass Conv. Bioref.

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King crab gills as a new source of chitin/chitosan and protein hydrolysates

Cited by 16 publications

References 47 publications

Mechanism of Heterogeneous Alkaline Deacetylation of Chitin: A Review

Mechanism of Heterogeneous Alkaline Deacetylation of Chitin: A Review

Chitin extraction from crab shells by two-step fermentation with Lacticanttacllus pantarum and Pseudomonas aeruginosa

Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa

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