Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Abstract

The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal progenitor cells and used to investigate bone-metabolism in vitro. Hereby, we studied whether and to what extent the conditionally immortalized cell line hFOB 1.19 can serve as a surrogate model for bone-marrow derived mesenchymal stromal cells (bmMSC). hFOB indeed exhibit specific characteristics reminiscent of bmMSC, as colony formation, migration capacity and the propensity to grow as multicellular aggregates. After prolonged culture, in contrast to the expected effect of immortalization, hFOB acquired a delayed growth rate. In close resemblance to bmMSC at increasing passages, also hFOB showed morphological abnormalities, enlargement and finally reduced proliferation rates together with enhanced expression of the cell cycle inhibitors p21 and p16. hFOB not only have the ability to undergo multilineage differentiation but portray several important aspects of human bone marrow mesenchymal stromal cells. Superior to primary MSC and osteoblasts, hFOB enabled the generation of continuous cell lines. These provide an advanced basis for investigating age-related dysfunctions of MSCs in an in vitro 3D-stem cell microenvironment.

A Poly-(ethylene glycol)-diacrylate 3D-Printed Micro-Bioreactor for Direct Cell Biological Implant-Testing on the Developing Chicken Chorioallantois Membrane

“…Post-print processing was validated by means of the ISO 10993-5:2009 standard assay on two different cell lines hFOB 1.19 and SaOS-2. We chose hFOB cells because they have been derived from fetal tissue and it could be shown, by us and others, that they represent a multipotent surrogate in vitro model for stromal mesenchymal stem cells [31,32]. We were able to show that the hFOB1.19 cells more sensitively indicate cytotoxicity than SaOS-2 cancer cells.…”

Low-Cost Devices for Three-Dimensional Cell Aggregation, Real-Time Monitoring Microscopy, Microfluidic Immunostaining, and Deconvolution Analysis

“…The materials are inexpensive and can be purchased without restrictions. Similar protocols have been communicated for specific areas of research and development in need of specifying standards of implementation for a broader use of this technology [ 24 ].…”

“…For aggregate-testing in a well, we employed a cell line of mesenchymal origin, human fetal osteoblasts, (hFOB 1.19), which exhibit strong cell interactions to form a stable aggregate [ 24 ]. In contrast, the Saos-2 cells refrained from aggregation.…”

Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and Regeneration

“…Human fetal osteoblasts have been used as a model osteoblastogenic cell line in the literature for many decades. 23,24 Moreover, studies involving an angiogenic marker and tube formation analyses have been using HUVECs as an endothelial model cell line. 25,26 Lately, human monocyte cell line, THP-1-ASC-GFPs or simply THP-1s, which can stably express apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) conjugated with a green fluorescent protein (GFP), has been widely used to monitor inflammasome production.…”

“…Effects of B on various effectors and genes were studied to characterize the effect of boron on a moderately crystalline cHA structure using human fetal osteoblasts (hFOBs), HUVECs, and human monocyte-like cells (THP-1s) for the first time. Human fetal osteoblasts have been used as a model osteoblastogenic cell line in the literature for many decades. , Moreover, studies involving an angiogenic marker and tube formation analyses have been using HUVECs as an endothelial model cell line. , Lately, human monocyte cell line, THP-1-ASC-GFPs or simply THP-1s, which can stably express apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) conjugated with a green fluorescent protein (GFP), has been widely used to monitor inflammasome production . THP-1s were also used in macrophage (MΦ) response analysis toward proinflammatory (M1) and prohealing (M2) directions …”