KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis

Gao, Zhen; Daneva, Anna; Salanenka, Yuliya; Durme, Matthias Van; Huysmans, Marlies; Lin, Zongcheng; Winter, Freya De; Vanneste, Steffen; Karimi, Mansour; Velde, Jan Van de; Vandepoele, Klaas; Walle, Davy Van de; Dewettinck, Koen; Lambrecht, Bart N.; Nowack, Moritz K.

doi:10.1038/s41477-018-0160-7

Cited by 85 publications

(86 citation statements)

References 63 publications

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“…Viability assays. The vital stains Fluorescein diacetate (FDA, Sigma) and Propidium Iodide (PI, Sigma) were applied simultaneously as reported by 52 . Cell viability was also determined in SCR::GFP plants as reported by 53 .…”

Section: Methodsmentioning

confidence: 99%

Laterals take it better – Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis

Ambastha

Friedmann

Leshem

2020

Sci Rep

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plant responses to salinity have been extensively studied over the last decades. Despite the vast accumulated knowledge, the ways Arabidopsis lateral roots (LR) cope with lethal salinity has not been fully resolved. Here we compared the primary root (pR) and the LR responses during events leading to lethal salinity (NaCl 200 mM) in Arabidopsis. We found that the PR and young LR responded differently to lethal salinity: While the PR died, emerging and young LR's remained strikingly viable. Moreover, "age acquired salt tolerance" (AAST) was observed in the PR. During the 2 days after germination (DAG) the PR was highly sensitive, but at 8 DAG there was a significant increase in the PR cell survival. nevertheless, the young LR exhibited an opposite pattern and completely lost its salinity tolerance, as it elongated beyond 400 µm. examination of several cell death signatures investigated in the young LR showed no signs of an active programmed cell death (pcD) during lethal salinity. However, Autophagic pcD (A-pcD) but not apoptosis-like pcD (AL-pcD) was found to be activated in the pR during the high salinity conditions. We further found that salinity induced NADPH oxidase activated ROS, which were more highly distributed in the young LR compared to the pR, is required for the improved viability of the LR during lethal salinity conditions. our data demonstrated a position-dependent resistance of Arabidopsis young LR to high salinity. This response can lead to identification of novel salt stress coping mechanisms needed by agriculture during the soil salinization challenge. The ongoing process of soil salinization negatively affects many plant species, including staple crops, imposing a major threat for global food production 1,2. Therefore, it is not surprising that over the last decades numerous studies have been dedicated to the understanding of plant responses to salt stress. These studies include those conducted from the organismic (whole plant) level to the cellular and molecular levels, deciphering both osmotic and ionic nature of salt 3-7. Since the root is the primary tissue that directly interacts with the rhizosphere's saline environment, special attention has been given to root tissue. In many of the past but also present studies, salt stress responses were determined in whole root samples without differentiating between the root's various developmental zones and cell types, mainly while being compared to the shoot 8,9. Nevertheless, it is well established by now that each of the root's different developmental zones responds to salt in a different manner. For example, the canonical Sodium/ Proton antiporter NHX1 was found in Arabidopsis roots to be expressed during salt stress in the elongation zone but excluded from the root tip meristem, whereas SOS1 exhibited the opposite expression pattern 10. Moreover, in a milestone study Dinneny et al. isolated several types of Arabidopsis root cells and showed that their transcriptomes differed significantly during salt stress 11. Therefore, it is necessary to spec...

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Section: Methodsmentioning

confidence: 99%

Laterals take it better – Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis

Ambastha

Friedmann

Leshem

2020

Sci Rep

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“…In comparison with leaf senescence, floral senescence might be more dependent on developmental than on environmental stimuli. For example, the lifespan of the stigma is tightly controlled by NAC transcription factors, which induce PCD to terminate flower receptivity for pollen (Gao et al, 2018). Also, pollination itself is an important trigger for floral organ senescence, often mediated by the phytohormone ethylene (Rogers, 2006(Rogers, , 2013.…”

Section: Floral Organ Senescencementioning

confidence: 99%

Plant proteases during developmental programmed cell death

Buono

Hudecek

Nowack

2019

Journal of Experimental Botany

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Proteases are among the key regulators of most forms of programmed cell death (PCD) in animals. Also in plants, many PCD processes have been associated with protease expression or activation. However, the functional evidence of the roles and actual modes of action of plant proteases in PCD remains surprisingly limited. In this review, we give an update on protease involvement in the context of developmentally regulated plant PCD. To illustrate the diversity of protease functions, we focus on several prominent developmental PCD processes, including xylem and tapetum maturation, suspensor elimination, endosperm degradation and seed coat formation, as well as plant senescence processes. Despite the substantial advance in the field, protease functions are still often only correlatively linked to developmental PCD, and the specific molecular roles of proteases in many developmental PCD processes remain to be elucidated.

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“…In this biological process, ORE1 levels are fine tuned by its polyubiquitination through NLA and PHO2, leading to subsequent ORE1 degradation, as well as the counteracting deubiquitination by UBP12 and UBP13, which stabilizes ORE1 and promotes senescence (Park et al, 2018(Park et al, , 2019. In addition to acting in leaf senescence, a partially redundant function of ORE1 has recently been documented in the stigma longevity by controlling the expression of programmed cell death-associated genes (Gao et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1

et al. 2020

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KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis

Cited by 85 publications

References 63 publications

Laterals take it better – Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis

Laterals take it better – Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis

Plant proteases during developmental programmed cell death

Calcium-Dependent Protein Kinase CPK1 Controls Cell Death by In Vivo Phosphorylation of Senescence Master Regulator ORE1

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