In this study, the effects on quality characteristics such as mass, length, width and color (L*, a* and b*) in blueberry (Vaccinium corymbosum L. cv. 'Bluecrop') fruit of biofilm (Parka TM ) sprayed in pre-harvest different regimes were investigated. The highest fruit mass was measured in T1, T3, T4 and T8 treatments at harvest, whereas all treatments at harvest +7 were similar. At harvest and harvest +7, the highest fruit length was obtained in T1 and T1 and T4 treatments, respectively. Fruit width was similar in all treatments at harvest and harvest +7 (except for T8). L* value of T3, T4, T5, T6 and T9 treatments was higher from T1 at harvest. On the contrary, it was similar in all treatments at harvest +7. It was observed that the a* and b* values of all treatments were similar. When harvesting periods are compared, fruit mass in T3 and T8; T3, T6, T7, T8, T9 and T10 in fruit length; fruit width in T3, T5, T6, T8 and T9; T5, T6 and T7 treatments in b* values was higher at harvest than at harvest +7. As a result, it was revealed that the biofilm could be a significant effect on the physical and color properties of the blueberry fruit.