Kısa Süreli PRID Uygulanan Düvelerde Bazı Oksidatif Stres Parametrelerinin Değerlendirilmesi

Abstract

The purpose of this study was to investigate the effect of short term administration of PRID on the levels of serum progesterone, nitric oxide (NO), malondialdehyde (MDA), total antioxidant capacity (TAC) and total oxidant capacity (TOC) in heifers. This purpose, 200 heifers were synchronized with the Cosynch-72 protocol in which progesterone was administered for 5 days. Blood was taken from the heifers 10 days prior to starting synchronization (day -10), the day the PRID was attached (day 0), the day the PRID was removed (day 5) and on the day of insemination (day 8). Blood taken on day -10 and day 0 were accepted as control values for the study. Serum progesterone, NO, MDA, TAC and TOC were measured on days 5 and 8. The difference in serum NO and MDA levels was found to be statistically significant on day 5 of the study (P<0.001). It was determined that the procedure caused a drop in the TAC level, while the TOC level was not affected. In conclusion, it was determined that short-term administration of PRID in heifers caused an increase in serum NO and MDA levels, a decrease in TAC level and had no effect on the TOC level. Keywords: Heifer, Nitric oxide, Oxidative Stress, PRID Kısa Süreli PRID Uygulanan Düvelerde Bazı Oksidatif Stres Parametrelerinin Değerlendirilmesi ÖzetBu çalışmada, düvelere kısa süreli PRID uygulamasının; serum progesteron, nitrik oksit (NO), malondialdehit (MDA), total antioksidant (TAK) ve oksidant kapasitesi (TOK) düzeyleri üzerine etkisinin araştırılması amaçlanmıştır. Bu amaçla, 200 adet düve 5 gün progesteron uygulanan Cosynch-72 protokolüyle senkronize edildi. Düvelerden senkronizasyona başlamadan 10 gün önce (-10. gün), PRID takılma günü (0. gün), PRID çıkarılma günü (5. gün) ve tohumlama günü (8. gün) kan alındı. Çalışmada -10 ve 0. gün alınan kanlar kontrol olarak değerlendirildi. Beş ve 8. günlerde serum progesteron, NO, MDA, TAK ve TOK değerleri araştırıldı. Çalışmada 5. gün serum NO ve MDA düzeyleri diğer günlerden istatistiksel olarak farklı olduğu tespit edildi (P<0,001). Uygulamanın TAK düzeyinde düşmeye neden olduğu, TOK değerini ise etkilemediği belirlendi. Sonuç olarak, düvelere kısa süreli PRID uygulamasının serum NO ve MDA düzeylerinde artışa neden olduğu, TAK düzeyinde düşmeye ve TOK değerini ise etkilemediği belirlendi.

Controlled Internal Drug Release Uygulaması İle Senkronizasyon Yapılarak Gebe ve Gebe Kalmayan Abaza Keçilerinde Oksidatif/Nitrozatif Stres, Leptin ve Progesteron Konsantrasyonlarının Karşılaştırılması

“…Oxidative stress is a serious problem which is being studied extensively in human and animals, especially in cattle, sheep and goats in conditions of sepsis, mastitis, metritis, retentio secundinarum and genital tract inflammation [6,19] . There are few studies about fertility and the oxidative stress created by P4 sources used intravaginally for estrus synchronization in small ruminants [4,8] .…”

“…These inserts are left in the vagina and can cause tissue damage and inflammation [5] . As a result, such applications can create stress for the animal [6] . A complex relationship has been reported between inflammation and reactive oxygen species (ROS) [7] .…”

“…Besides protecting vascular integrity and preventing leukocytes from attaching to endothelial cells and the proliferation of smooth muscle cells, endothelium-derived NO also acts to inhibit thrombocyte adhesion and aggregation [10] . A study conducted on dairy heifers reported that NO and MDA levels increased after application of intravaginal inserts and total antioxidant capacity (TAC) was decreased [6] .…”

Brusellozisli Sığırlarda Oksidatif Stres İndeksi ve Lipid Profilinin İncelenmesi

“…In the case of inflammation or various infections, antioxidant defense gets weakens, and increased reactive oxygen species react with macromolecules, including proteins, lipids and DNA; and ultimately, lead to oxidative damage [8,23,24] . In the current study, parameters of oxidative stress were measured to determine the severity of the cellular damage and the effectiveness of the treatment likewise reported formerly [5,6,25] .…”

“…Cells and tissues have antioxidant systems that inhibit radical products and reactions. Studies have demonstrated altered total oxidant (TOC) and total antioxidant capacity (TAC), or oxidative stress index (OSI) in case of local and systemic inflammation or infection [5,7,8] .…”