Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchus labrax)

Migaud, Hervé; Ismail, Rania F.; Cowan, Mairi; Davie, Andrew

doi:10.1016/j.ygcen.2012.07.033

Cited by 48 publications

(27 citation statements)

References 92 publications

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“…The tissue expression of kissr genes, and those of their cognate ligands kiss1 and kiss2, in the brain and the gonads of sea bass, medaka and zebrafish is fully compatible with their putative roles in fish reproduction (Felip et al (2009) and this study). In this respect, the mRNA expression profiles of kissr and kiss genes in the brain of sea bass suggested that these two kisspeptin systems might be involved in segregated functions of neuroendocrine signaling that control early or late events of gametogenesis in this species (Migaud et al 2012, Alvarado et al 2013. Moreover, the expression profiles of two kisspeptin genes and their receptors in the gonads of adult male sea bass support the role of kisspeptins in the regulation of late stages of spermatogenesis in this species, as reported in other teleosts.…”

Section: Discussionmentioning

confidence: 99%

“…It is an economically important farmed fish that still exhibits reproduction-related problems, such as the appearance of early puberty, and the mechanisms underlying this process merit further investigation ). Previous studies in this species have found that the sea bass has two distinct kiss genes, kiss1 and kiss2 (Felip et al 2009), whose mRNA levels in the brain change significantly in relation to the reproductive status of the animals (Migaud et al 2012, Alvarado et al 2013. The distribution of kiss1-and kiss2-expressing cells and their receptors in the brain of male and female sea bass undergoing their first sexual maturation has revealed that no sexual differences in the localization of the kisspeptin systems exist in this species (Escobar et al 2013a,b).…”

Section: Introductionmentioning

confidence: 93%

“…Expression levels of kiss and kissr in the brain and, to a lesser extent, in the gonad have been analyzed in several non-mammalian species, including amphibians (Chianese et al 2013) and fish (Mohamed et al 2007, Nocillado et al 2007, Biran et al 2008, Filby et al 2008, van Aerle et al 2008, Kitahashi et al 2009, Mechaly et al 2009, Migaud et al 2012, Alvarado et al 2013, Ohga et al 2013, suggesting a putative role for kisspeptins in controlling reproduction. Moreover, in vivo studies in some teleosts have found that the systemic administration of kisspeptin forms induces detectable biological responses at brain and pituitary levels, with potencies depending on the species (Filby et al 2008, Felip et al 2009, Kitahashi et al 2009, Li et al 2009, Shi et al 2010, Espigares et al 2015.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the expression of kissr genes in brain, pituitary and gonads in adult and juvenile fish during the reproductive period is explored. The expression study of kiss and kissr genes was extended to the different stages of gonad maturation in adult male sea bass as recent findings have given evidence for a differential involvement of the two kisspeptin systems in controlling gametogenesis in this species (Migaud et al 2012, Alvarado et al 2013. Furthermore, melatonin administration has been shown to elicit changes in the expression of kisspeptin/gnrh system genes in the brain that appear to mirror disturbances in spermatogenesis (Alvarado et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

et al. 2015

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Two forms of kiss gene (kiss1 and kiss2) have been described in the teleost sea bass. This study assesses the cloning and characterization of two Kiss receptor genes, namely kissr2 and kissr3 (known as gpr54-1b and gpr54-2b, respectively), and their signal transduction pathways in response to Kiss1 and Kiss2 peptides. Phylogenetic and synteny analyses indicate that these paralogs originated by duplication of an ancestral gene before teleost specific duplication. The kissr2 and kissr3 mRNAs encode proteins of 368 and 378 amino acids, respectively, and share 53.1% similarity in amino acid sequences. In silico analysis of the putative promoter regions of the sea bass Kiss receptor genes revealed conserved flanking regulatory sequences among teleosts. Both kissr2 and kissr3 are predominantly expressed in brain and gonads of sea bass, medaka and zebrafish. In the testis, the expression levels of sea bass kisspeptins and Kiss receptors point to a significant variation during the reproductive cycle. In vitro functional analyses revealed that sea bass Kiss receptor signals are transduced both via the protein kinase C and protein kinase A pathway. Synthetic sea bass Kiss1-15 and Kiss2-12 peptides activated Kiss receptors with different potencies, indicating a differential ligand selectivity. Our data suggest that Kissr2 and Kissr3 have a preference for Kiss1 and Kiss2 peptides, respectively, thus providing the basis for future studies aimed at establishing their physiologic roles in sea bass. . To date, a single ligand (Kiss) and receptor (Gpr54 or Kissr) have been demonstrated to exist in placental mammals (human, opossum) and reptiles, while mammalian monotrems (platypus) possess two forms of Kiss and Kissr genes. Contrasting situations are found in other tetrapodes, such as amphibians, which have three kiss and kissr genes, whereas the kisspeptin system is absent in birds, although a recent investigation into the kisspeptin system in avian lineages provides molecular evidence of the presence of a kiss2-like gene that degenerated and lost its function (Pasquier et al. 2014b). All in all, advances in genome sequencing and comparative genomics in several groups occupying relevant phylogenetic positions have led to the identification of four kissr and three kiss genes in the coelacanth (Sarcopterygian), while in teleosts at least one kiss and kissr is known to be present in all the species investigated to date. Nevertheless, a second gene for kisspeptin and its receptor has been observed in the genomes of other fish species (Biran et al. 2008, Akazome et al. 2010, Oakley et al. 2010, Um et al. 2010, Zohar et al. 2010, Kim et al. 2012, Pasquier et al. 2012a,b, Tena-Sempere et al. 2012. So far, the occurrence of three kissr and two kiss paralogous genes has been reported in an early group of teleosts, the Elopomorphs (European eel, Anguilla anguilla) (Pasquier et al. 2012a,b). In the spotted gar, Lepisosteus oculatus (Actinopterygian, Ginglymode), four kissr and two kiss have been identified, while four kiss but n...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

et al. 2015

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“…Since the reproductive physiology of European sea bass (Dicentrarchus labrax, Linnaeus) is well documented (Carrillo et al, 1995, this species has emerged as an interesting teleost model to investigate the differential involvement of the two kisspeptin systems in controlling fish reproduction (Alvarado et al, 2013;Escobar et al, 2013a,b;Felip et al, 2009Felip et al, , 2015Migaud et al, 2012). This species exhibits a clear stimulation of gonadotropin release following systemic kisspeptin administration , and more recently, intracerebroventricular injections of Kiss2 have demonstrated the involvement of this peptide in neuroendocrine regulation of gonadotroph activity .…”

Section: Introductionmentioning

confidence: 99%

Actions of sex steroids on kisspeptin expression and other reproduction-related genes in the brain of the teleost fish european sea bass

Alvarado

Servili

Molés

et al. 2016

Journal of Experimental Biology

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Kisspeptins are well known as mediators of the coordinated communication between the brain-pituitary axis and the gonads in many vertebrates. To test the hypothesis that gonadal steroids regulate kiss1 and kiss2 mRNA expression in European sea bass (a teleost fish), we examined the brains of gonad-intact (control) and castrated animals, as well as castrated males (GDX) and ovariectomized females (OVX) that received testosterone (T) and estradiol (E 2 ) replacement, respectively, during recrudescence. In GDX males, low expression of kiss1 mRNA is observed by in situ hybridization in the caudal hypothalamus (CH) and the mediobasal hypothalamus (MBH), although hypothalamic changes in kiss1 mRNA levels were not statistically different among the groups, as revealed by real-time PCR. However, T strongly decreased kiss2 expression levels in the hypothalamus, which was documented in the MBH and the nucleus of the lateral recess (NRLd) in GDX T-treated sea bass males. Conversely, it appears that E 2 evokes low kiss1 mRNA in the CH, while there were cells expressing kiss2 in the MBH and NRLd in these OVX females. These results demonstrate that kisspeptin neurons are presumably sensitive to the feedback actions of sex steroids in the sea bass, suggesting that the MBH represents a major site for sex steroid actions on kisspeptins in this species. Also, recent data provide evidence that both positive and negative actions occur in key factors involved in sea bass reproductive function, including changes in the expression of gnrh-1/gonadotropin, cyp19b, er and ar genes and sex steroid and gonadotropin plasma levels in this teleost fish.

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Reproductive Biology of Teleost Fishes

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Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchus labrax)

Cited by 48 publications

References 92 publications

Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

Actions of sex steroids on kisspeptin expression and other reproduction-related genes in the brain of the teleost fish european sea bass

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