Kisspeptin regulates the development of caprine primordial follicles <i>in vitro</i>

Magamage, M. P. S.; Sathagopam, Sriravali; Avula, Kiran; Madushanka, D. N. N.; Velmurugan, Sathya

doi:10.12750/jarb.36.1.51

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…It has been declared that kisspeptin potentiates the induction of ovarian follicle maturation and estradiol production by promoting FSH synthesis ( Hagen et al ., 2014 ; Rather et al ., 2016 ). The line with this notion, some published papers emphasized the role of this antioxidant in improving follicle development and maturity ( Taniguchi et al ., 2017 ; Magamage et al ., 2021 ). In this regard, a study revealed that the administration of kisspeptin increases the number of preovulatory follicles and raises the plasma AMH secreted by secondary follicles ( Cao et al ., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The effect of kisspeptin on the maturation of human ovarian follicles in culture following vitrification-thawing processes

Tavakoli,

Azar,

Taghizabet

et al. 2023

JBRA

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Objective Ovarian cryopreservation is one of the effective methods to preserve fertility for cancer patients. Still, this approach has some problems, namely ROS, resulting in adverse effects on oocytes and ovarian follicles. Kisspeptin as an antioxidant to control ovarian function, directly or indirectly. In this study, the effect of kisspeptin on follicle maturation was evaluated in culture following ovarian cryopreservation. Methods Ovarian tissue samples of women between 20 and 35 years old (n=12) were laparoscopically collected. The samples were randomly divided into four groups: 1) control, 2) vitrification, 3) vitrified+1µM kisspeptin, and 4) vitrified+10µM kisspeptin. After vitrification and thawing processes, the tissues were cultured in DMEM medium for 7 days. H&E staining for histological evaluation, Real-Time PCR for GDF9 and BMP15 gene expression, and immunohistochemical staining for GDF9 and BMP15 protein expression were performed. Results In the vitrification group, ovarian tissue morphology was incoherent, and more primordial follicles than other follicle types were found. The expression of GDF9 and BMP15 genes and proteins were significantly decreased in this group compared with other groups ( p <0.05). In the vitrification groups with kisspeptin (1 and 10 µM), the number of primary and secondary follicles was more than in the vitrification group. Besides, the expression of these genes and proteins was dramatically elevated in the vitrification groups with kisspeptin compared to the vitrification group alone ( p <0.05). Conclusions It seems that kisspeptin is an effective substance to improve the quality of the human ovarian cryopreservation medium by improving follicle maturation.

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Section: Discussionmentioning

confidence: 99%

The effect of kisspeptin on the maturation of human ovarian follicles in culture following vitrification-thawing processes

Tavakoli,

Azar,

Taghizabet

et al. 2023

JBRA

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Kisspeptin decreases the adverse effects of human ovarian vitrification by regulating ROS-related apoptotic occurrences

Tavakoli,

Aliakbari,

Soleimani Mehranjani

2023

Zygote

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Summary Kisspeptin is characterized as a neuropeptide with a pivotal function in female and male infertility, and its antioxidant properties have been demonstrated. In this study, the effects of kisspeptin on the improvement of the vitrification and thawing results of human ovarian tissues were investigated. In this work, 12 ovaries from patients who underwent hysterectomy were collected laparoscopically, and then 32 samples from each of their tissues were taken. Haematoxylin and eosin (H&E) staining was performed to check the normality of the ovarian tissue and, subsequently, the samples were allocated randomly into four groups, including: (1) fresh (control), (2) vitrification, (3) vitrified + 1 μM kisspeptin, and (4) vitrified + 10 μM kisspeptin groups. After vitrification, thawing, and tissue culture processes, H&E staining for tissue quality assessment, terminal deoxynucleotidyl transferase dUTP nick end labelling assay for apoptosis evaluation, and malondialdehyde (MDA), superoxide dismutase (SOD), and ferric reducing ability of plasma tests for oxidative stress appraisal were carried out. Our histological results showed incoherency of ovarian tissue morphology in the vitrification group compared with other groups. Other findings implicated increased apoptosis rate and MDA concentration and reduced SOD activity and total antioxidant capacity (TAC) in the vitrification group compared with the control group (P < 0.05). Moreover, decreased apoptosis rate and MDA concentration, and increased TAC and SOD function were observed in the vitrification with kisspeptin groups (1 μM and 10 μM) compared with the vitrified group (P < 0.05). Our reports express that kisspeptin is an effective agent to overcome the negative effects of vitrification by regulating reactive oxygen species-related apoptotic processes.

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Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors

Divya Sri,

Harsha Lekha,

Reddy

et al. 2023

Zygote

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Summary The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the in vitro development of preantral follicles (PFs) and (2) evolution of KP receptor gene (KISS1R) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0–100 µg/ml) in the culture medium of PFs for 6 days. The cumulus–oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development in vitro. The expression of the kisspeptin receptor gene at each development stages of in vivo developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and in vitro cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The KISS1R gene was expressed in follicular cells and oocytes at all the development stages of both in vivo developed and in vitro cultured follicles. Higher KISS1R gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.

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Kisspeptin regulates the development of caprine primordial follicles in vitro

Cited by 3 publications

References 27 publications

The effect of kisspeptin on the maturation of human ovarian follicles in culture following vitrification-thawing processes

The effect of kisspeptin on the maturation of human ovarian follicles in culture following vitrification-thawing processes

Kisspeptin decreases the adverse effects of human ovarian vitrification by regulating ROS-related apoptotic occurrences

Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors

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