KIT-SNAP-tag/cell membrane chromatography model coupled with liquid chromatography–mass spectrometry for anti-GIST compound screening from Evodia rutaecarpa

Feng, Jingting; Jiang, Yuhan; Liu, Sihan; Deng, Linge; Lv, Yanni; Chen, Nanzheng; Han, Shengli

doi:10.1007/s00216-024-05148-5

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…The targeting ability of the membrane-targeted unit depends on the structures of the plasma membrane, which contains plasma membrane proteins and the phospholipid bilayer structure . Various approaches can be used to label plasma membrane proteins, including fusion protein tags, aptamers, small molecular ligands, and antibodies. − However, the indicators designed based on proteins are usually complicated, and the raw material cost is expensive. To date, most luminescence indicators for plasma membrane staining have utilized ligands and cholesterol groups as membrane-targeted units.…”

Section: Introductionmentioning

confidence: 99%

Tracking Plasma Membrane Damage Using a Ruthenium(II) Complex Phosphorescent Indicator Paired with Cholesterol

Fa,

Gao,

Zhang

et al. 2024

Inorg. Chem.

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Long-term in situ plasma membrane-targeted imaging is highly significant for investigating specific biological processes and functions, especially for the imaging and tracking of apoptosis processes of cells. However, currently developed membrane probes are rarely utilized to monitor the in situ damage of the plasma membrane. Herein, a transition-metal complex phosphorescent indicator, Ru-Chol, effectively paired with cholesterol, exhibits excellent properties on staining the plasma membrane, with excellent antipermeability, good photostability, large Stokes shift, and long luminescence lifetime. In addition, Ru-Chol not only has the potential to differentiate cancerous cells from normal cells but also tracks in real time the entire progression of cisplatin-induced plasma membrane damage and cell apoptosis. Therefore, Ru-Chol can serve as an efficient tool for the monitoring of morphological and physiological changes in the plasma membrane, providing assistance for drug screening and early diagnosis and treatment of diseases, such as immunodeficiency, diabetes, cirrhosis, and tumors.

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